TFPI (BC015514) Human Untagged Clone
CAT#: SC123622
TFPI (untagged)-Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (cDNA clone MGC:9251 IMAGE:3902987), complete cds
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | TFPI |
Synonyms | EPI; LACI; TFI; TFPI1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for BC015514 edited
AACAGACAGCAGCGACTTTAGGCTGGATAATAGTCAAATTCTTACCTCGCTCTTTCACTG CTAGTAAGATCAGATTGCGTTCTTTCAGTTACTCTTCAATCGCCAGTTTCTTGATCTGCT TCTAAAAGAAGAAGTAGAGAAGATAAATCCTGTCTTCAATACCTGGAAGGAAAAACAAAA TAACCTCAACTCCGTTTTGAAAAAAACATTCCAAGAACTTTCATCAGAGATTTTACTTAG ATGATTTACACAATGAAGAAAGTACATGCACTTTGGGCTTCTGTATGCCTGCTGCTTAAT CTTGCCCCTGCCCCTCTTAATGCTGATTCTGAGGAAGATGAAGAACACACAATTATCACA GATACGGAGTTGCCACCACTGAAACTTATGCATTCATTTTGTGCATTCAAGGCGGATGAT GGCCCATGTAAAGCAATCATGAAAAGATTTTTCTTCAATATTTTCACTCGACAGTGCGAA GAATTTATATATGGGGGATGTGAAGGAAATCAGAATCGATTTGAAAGTCTGGAAGAGTGC AAAAAAATGTGTACAAGAGATAATGCAAACAGGATTATAAAGACAACATTGCAACAAGAA AAGCCAGATTTCTGCTTTTTGGAAGAAGATCCTGGAATATGTCGAGGTTATATTACCAGG TATTTTTATAACAATCAGACAAAACAGTGTGAACGTTTCAAGTATGGTGGATGCCTGGGC AATATGAACAATTTTGAGACACTGGAAGAATGCAAGAACATTTGTGAAGATGGTCCGAAT GGTTTCCAGGTGGATAATTATGGAACCCAGCTCAATGCTGTGAATAACTCCCTGACTCCG CAATCAACCAAGGTTCCCAGCCTTTTTGTTACAAAAGAAGGAACAAATGATGGTTGGAAG AATGCGGCTCATATTTACCAAGTCTTTCTGAACGCCTTCTGCATTCATGCATCCATGTTC TTTCTAGGATTGGATAGCATTTCATGCCTATGTTAATATTTGTGCTTTTGGCATTTCCTT AATATTTATATGTATACGTGATGCCTTTGATAGCATACTGCTAATAAAGTTTTAATATTT ACATGCAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for BC015514 unedited
GTTCAGATTTGTATACGACTCACTATAGGCGGCCGCGATTCCCGGGNAATCGTCGACCCA CGCGTCCGAACANACAGCAGCGACTTTAGGCTGGATAATAGTCAAATTCTTACCTCGCTC TTTCACTGCTAGTAAGATCAGATTGCGTTCTTTCAGTTACTCTTCAATCGCCAGTTTCTT GATCTGCTTCTAAAAGAAGAAGTAGAGAAGATAAATCCTGTCTTCAATACCTGGAAGGAA AAACAAAATAACCTCAACTCCGTTTTGAAAAAAACATTCCAAGAACTTTCATCAGAGATT TTACTTAGATGATTTACACAATGAAGAAAGTACATGCACTTTGGGCTTCTGTATGCCTGC TGCTTAATCTTGCCCCTGCCCCTCTTAATGCTGATTCTGAGGAAGATGAAGAACACACAA TTATCACAGATACGGAGTTGCCACCACTGAAACTTATGCATTCATTTTGTGCATTCAAGG CGGATGATGGCCCATGTAAAGCAATCATGAAAAGATTTTTCTTCAATATTTTCACTCGAC AGTGCGAAGAATTTATATATGGGGGATGTGAAGGAAATCAGAATCGATTTGAAAGTCTGG AAGAGTGCAAAAAAATGTGTACAAGAGATAATGCAAACAGGATTATAAAGACAACATTGC AACAAGAAAAGCCAGATTTCTGCTTTTTGGAAGAAGATCCTGGAATATGTCGAGGTTATA TTACCAGGTATTTTTATAACAATCAGACAAAACAGTGTGAACGTTTNCAAGTATGGTGGA TGCCTGGGCAATATGAACAATTTTGAGACACTGGAAGAATGCAAGAACATTTGTGAAGAT GGTCCGAATGGTTTCCAGGTGGATAATTATGGAACCCAGCTCAATGCTGTGAATAACTCC CTGACTCCGCATCAACCAGGTTCCNAGNCTTTTTGTTACAAAAGAG |
Restriction Sites | Please inquire |
ACCN | BC015514 |
Insert Size | 1108 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC015514.1, AAH15514.1 |
RefSeq Size | 1108 bp |
Locus ID | 7035 |
Cytogenetics | 2q32.1 |
Protein Families | Secreted Protein |
Protein Pathways | Complement and coagulation cascades |
Gene Summary | 'This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016]' |
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