LIM kinase 2 (LIMK2) (BC013051) Human Untagged Clone

CAT#: SC123647

LIMK2 (untagged)-Human LIM domain kinase 2 (cDNA clone MGC:8917 IMAGE:3861412), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LIMK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC013051 edited
  GGGAGGAGCCATCCCAGCCATGAGCCCCTGTGGGAATCTGCTGGGGGCCAAGTGGCCTGG
  AGTCCTCAGGCTCCCGCAGCTGCTCCGGAGGGAGAGGTGAGCTCAGGGCAGCCTGCCTGC
  AGCCAGAGGTGCCGGGAGCCCCGGGCCTGTCATGGTGGCCATCTACAGCCGGCCTGAGGC
  AGTCACAGACGGATTTGCAGCTGAGCCTGTCTATCTGGTGTGGGAAGAAGATGGGGAGTT
  ACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCAGAATGCCAGG
  ATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACT
  GGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTATGG
  TGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGCTGCAAGGTGATCA
  TTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTACTGTGGGAAGTGCC
  ACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAGTCTGTTCAGGAGC
  AGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGCAGGCGGGGCT
  TCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGAAAGAGG
  TCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGGGACCGCATCC
  TGGAGATCAATGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTGGAGGATGCAATTA
  GCCAGACGAGCCAGACACTTCAGCTGTTGATTGAACATGACCCCGTCTCCCAACGCCTGG
  ACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACATGCAGAATGCCGGACACCCCCACG
  CCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGGAGACGTTCCCTAA
  GGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAGCCCCTGCTGT
  TCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTATTCACAGCAGA
  TCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGG
  CTATCAAGGTGACACACAAAGCCACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGAT
  GTGATGAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACC
  ACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAAGGATAAGAAGCTGAACCTCCTGA
  CAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATGGATCCGTTCCCCT
  GGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTATTTGCACTCTA
  TGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTGGACAAGACTG
  TGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAAAGGGCCCCCA
  TGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGACCGCAAGAAGCGCTACA
  CGGTGGTGGGAAACCCCTACTGGATGGCCCCTGAGATGCTGAACGGAAAGAGCTATGATG
  AGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTGTGAGATCATTGGGCAGGTGTATG
  CAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTGAAGCTTTTCTGGG
  AGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCCATCTGCTGCA
  GACTGGAGCCTGAGAGCAGAGCCCCCCCCGGGGCCGCAGGAGAGGGCCCGGGCTGCGCGG
  ATGATGAGGGCCCAGTGAGGCGCCAAGGGAAGGTCACCATCAAGTATGACCCCAAGGAGC
  TACGGAAGCACCTCAACCTAGAGGAGTGGATCCTGGAGCAGCTCACGCGCCTCTACGACT
  GCCAGGAAGAGGAGATCTCAGAACTAGAGATTGACGTGGATGAGCTCCTGGACATGGAGA
  GTGACGATGCCTGGGCTTCCAGGGTCAAGGAGCTGCTGGTTGACTGTTACAAACCCACAG
  AGGCCTTCATCTCTGGCCTGCTGGACAAGATCCGGGCCATGCAGAAGCTGAGCACACCCC
  AGAAGAAGTGAGGGTCCCCGACCCAGGCGAACGGTGGCTCCCATAGGACAATCGCTACCC
  CCCGACCTCGTAGCAACAGCAATACCGGGGGACCCTGCGGCCAGGCCTGGTTCCATGAGC
  AGGGCTCCTCGTGCCCCTGGCCCAGGGGTCTCTTCCCCTGCCCCCTCAGTTTTCCACTTT
  TGGATTTTTTTATTGTTATTAAACTGATGGGACTTTGTGTTTTTATATTGACTCTGCGGC
  ACGGGCCCTTTAATAAAGCGAGGTAGGGTACGCCTTTGGTGCAGCTCAAAAAAAAAAAAA
  AAAATGATTTCCAGCGGTCCACATTAGAGTTGAAATTTTCTGGTGGGAGAATCTATACCT
  TGTTCCTTTATAGGCCAAGGACCGCAGTCCTTCAGTAACACCAGTGTAAAAGCTTGAGGA
  GAAATTGTGAAGCTACACAGTATTTGTTTTCTAATACCTCTTGTCATTCTAAATATCTTT
  AATTTATTAAAAAATATATATATACAGTAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC013051 unedited
  CCGAATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCCCGGGATATCGTCGACC
  CACGCGTCCGGGGAGGAGCCATCCCAGCCATGAGCCCCTGTGGGAATCTGCTGGGGGCCA
  AGTGGCCTGGAGTCCTCAGGCTCCCGCAGCTGCTCCGGAGGGAGAGGTGAGCTCAGGGCA
  GCCTGCCTGCAGCCAGAGGTGCCGGGAGCCCCGGGCCTGTCATGGTGGCCATCTACAGCC
  GGCCTGAGGCAGTCACAGACGGATTTGCAGCTGAGCCTGTCTATCTGGTGTGGGAAGAAG
  ATGGGGAGTTACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCA
  GAATGCCAGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCC
  AAGGACTACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGG
  CCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGCTGC
  AAAGTGATCATTGAGGATGGNGATGCATATGCACTGGTGCAGCATGCCACCCTCTACTGT
  GGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCACAGAGTCTG
  TTCAGGAGCAGCTGCCCTACTTTGTCACGCTCATCTTCATGGCCGGCCACCATGTAGGGG
  CAGCGGGGCCTCCTCCGGGTCGTGGGAGAGGGCCTGGCTCAACTACCCCACCCCTGGGCA
  GGTGAAAGAGTCAACCGGAATGCACTTAGTCCCCACAATCGAAACGCCTCCCACCCGGGG
  ACCCCATCCTGGAAACCAAGGGACCCCCGTCCCCACCTTTCGGGGGAGGAGGTGGGGGGT
  CAATTAGCCAGAAAGCCCCACACTTAGCCGTGGAGTGAAGAGAAC
  
• Restriction Sites: Please inquire
• ACCN: BC013051
• Insert Size: 2805 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013051.1, AAH13051.1
• RefSeq Size: 2805 bp
• Locus ID: 3985
• Cytogenetics: 22q12.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: 'There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'