OGDH (BC009580) Human Untagged Clone

CAT#: SC123700

OGDH (untagged)-Homo sapiens, Similar to oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide), clone MGC:14605 IMAGE:4046929, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OGDH
• Synonyms: 2-oxoglutarate dehydrogenase; AKGDH; E1k; H_DJ0691F11_gi16307008.sp_cds.1; OGDC; oxoglutarate (alpha-ketoglutarate) dehydrogenase (lip; oxoglutarate decarboxylase'; oxoglutarate dehydrogenase (lipoamide); oxoglutarate dehydrogenase (succinyl-transferring)
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC009580 edited
  CCGAGAGTTTGGAGCCCCGGAGTGGGGTGTCGGCGCCTCATTCGGGTGGAGCTGAGCCGG
  AGACAGGCAGTTGTGAAAAACTTCAGGACAAAAATGTTTCATTTAAGGACTTGTGCTGCT
  AAGTTGAGGCCATTGACGGCTTCCCAGACTGTTAAGACATTTTCACAAAACAGACCAGCA
  GCAGCTAGGACATTTCAACAGATTCGGTGCTATTCTGCACCTGTTGCTGCTGAGCCCTTT
  CTCAGTGGGACTAGTTCGAACTATGTGGAGGAGATGTACTGTGCTTGGCTGGAAAACCCC
  AAAAGTGTACATAAGTCATGGGACATTTTTTTTCGCAACACGAATGCCGGAGCCCCACCG
  GGCACTGCCTACCAGAGTCCCCTTCCCCTGAGCCGAGGCTCCCTGGCTGCTGTGGCCCAT
  GCACAGTCCCTGGTAGAAGCACAGCCCAACGTGGACAAGCTCGTGGAGGACCACCTGGCA
  GTGCAGTCGCTCATCAGGGCATATCAGATACGAGGGCACCATGTAGCACAGCTGGACCCC
  CTGGGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTGACATTATCTCATCCACA
  GACAAACTTGGGTTCTATGGCCTGGATGAGTCTGACCTCGACAAGGTCTTCCACTTGCCC
  ACCACCACTTTCATCGGGGGACAGGAATCAGCACTTCCTCTGCGGGAGATCATCCGTCGG
  CTGGAGATGGCCTACTGCCAGCATATTGGGGTGGAGTTCATGTTCATCAATGACCTGGAG
  CAGTGCCAGTGGATCCGGCAGAAGTTTGAGACCCCTGGGATCATGCAGTTCACAAATGAG
  GAGAAACGGACCCTGCTGGCCAGGCTTGTGCGGTCCACCAGGTTTGAGGAGTTCCTACAG
  CGGAAGTGGTCCTCTGAGAAGCGCTTTGGTCTAGAAGGCTGCGAGGTACTGATCCCTGCC
  CTCAAGACCATCATTGACAAGTCTAGTGAGAATGGCGTGGACTACGTGATCATGGGCATG
  CCACACAGAGGGCGGCTGAACGTGCTTGCAAATGTCATCAGGAAGGAGCTGGAACAGATC
  TTCTGTCAATTCGATTCAAAGCTGGAGGCAGCTGATGAGGGCTCCGGAGATGTGAAGTAC
  CACCTGGGCATGTATCACCGCAGGATCAATCGTGTCACCGACAGGAACATTACCTTGTCC
  TTGGTGGCCAACCCTTCCCACCTTGAGGCCGCTGACCCCGTGGTGATGGGCAAGACCAAA
  GCCGAACAGTTTTACTGTGGCGACACTGAAGGGAAAAAGGTAAGGCCCAGAGAGAGGCGT
  GCAAGGCAGATCGTCAAGGCCCCATGTTCCAGCATGGAGTTCCGCTCACCAACATAACCC
  AGAGCCCTGGGTGCATCTAGACTTTAAAAAAATATTTAAAGTCGGCCGGGCGCAGTGTCT
  CACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCAGATCACCTGAGTTCGGGAG
  TTGGAGACCAGCCTGACCAACATGGAGAAACTCCATCTCTACTAAAAATACAAAATTAGC
  TGGGCGTGGTGGCGCGCGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCG
  CTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCGAGATTACGCCATTGCACTCCAGCCTG
  GGCCAACAAGAGCGAAACTCTGTCTCAAAGAAAAAAATAAATAAATAAAAAATATATGTA
  TATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC009580 unedited
  NNCCAGACACATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCCATTACGGCCGG
  GGCCGAGAGTTTGGAGCCCCGGAGTGGGGTGTCGGCGCCTCATTCGGGTGGAGCTGAGCC
  GGAGACAGGCAGTTGTGAAAAACTTCAGGACAAAAATGTTTCATTTAAAGACTTGTGCTG
  CTAAGTTGAGGCCATTGACGGCTTCCCAGACTGTTAAGACATTTTCACAAAACAGACCAG
  CAGCAGCTAGGACATTTCAACAGATTCGGTGCTATTCTGCACCTGTTGCTGCTGAGCCCT
  TTCTCAGTGGGACTAGTTCGAACTATGTGGAGGAGATGTACTGTGCTTGGCTGGAAAACC
  CCAAAAGTGTACATAAGTCATGGGACATTTTTTTTCGCAACACGAATGCCGGAGCCCCAC
  CGGGCACTGCCTACCAGAGTCCCCTTCCCCTGAGCCGAGGCTCCCTGGCTGCTGTGGCCC
  ATGCACAGTCCCTGGTAGAAGCACAGCCCAACGTGGACAAGCTCGTGGAGGACCACCTGG
  CAGTGCAGTCGCTCATCAGGGCATATCAGATACGAGGGCACCATGTAGCACAGCTGGACC
  CCCTGAGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTGACATTATCTCATCCA
  CAGACAAACTTGAGTTCTATGGCCTGGATGAGTCTGACCTCGACAGGTCTTCCACTTGCC
  CACCACCACTTTCATCGGGGGACAGGAATCAGCACTTCCTCTGCGGNAGATCATCCGTCG
  GCTGGAGATGGCCTACTGCCAGCATATTGAGGGTGGAGTTCATGTTNCATCATGACCCTN
  AGCANTGCCCATTGGATCCGGCAGAAGTTTGAGACCCCTGGGATCATGCAGTTCACN
  
• Restriction Sites: Please inquire
• ACCN: BC009580
• Insert Size: 1775 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009580.1, AAH09580.1
• RefSeq Size: 1775 bp
• Locus ID: 4967
• Cytogenetics: 7p13
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Lysine degradation, Metabolic pathways, Tryptophan metabolism
• Gene Summary: 'This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]'