MDFI (BC007836) Human Untagged Clone

CAT#: SC123736

MDFI (untagged)-Human MyoD family inhibitor (cDNA clone MGC:14090 IMAGE:4110841), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MDFI
• Synonyms: I-MF; I-mfa; inhibitor of MyoD family a; MyoD family inhibitor; OTTHUMP00000016375; OTTHUMP00000196414
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC007836 edited
  GAGAAAGCAGCGAGTGAGAGGGGAAGGGGCGCCAGGCGAGCACCCGGGAGCCAGCGGGAC
  CTGGGCAGGGGCGCCCGGAGCAGGCGCGCATGGCGGGCCCCGCGCGGGGATCCGGCTGGA
  AGAGAGCGTAGCACGGCTCGCACGAGTCCGGGGCCGATGTACCAGGTGAGCGGCCAGCGC
  CCCTCTGGCTGCGACGCGCCCTATGGAGCCCCCAGCGCAGCCCCGGGCCCAGCCCAGACC
  CTATCCCTCCTTCCTGGGCTGGAGGTAGTAACAGGATCCACTCACCCTGCGGAGGCAGCA
  CCAGAGGAGGGCTCCCTGGAGGAGGCGGCAACCCCCATGCCCCAAGGCAATGGCCCTGGC
  ATCCCCCAGGGCCTGGACAGCACTGACCTCGACGTCCCCACAGAAGCTGTGACATGCCAG
  CCTCAGGGGAACCCCTTGGGCTGCACCCCACTTCTGCCGAATGACTCTGGCCACCCCTCA
  GAGCTGGGCGGCACCAGACGGGCGGGGAATGGTGCCCTGGGTGGCCCCAAGGCCCACCGG
  AAGTTGCAGACACACCCATCTCTCGCCAGCCAGGGCAGCAAGAAGAGTAAGAGCAGCAGC
  AAATCCACCACCTCCCAGATCCCCCTCCAGGCACAGGAAGACTGCTGTGTCCACTGCATC
  CTGTCCTGCCTGTTCTGCGAGTTCCTGACGCTGTGCAACATCGTCCTGGACTGCGCCACC
  TGTGGCTCCTGCAGCTCGGAGGACTCGTGCCTCTGCTGCTGCTGCTGTGGCTCTGGCGAG
  TGTGCCGACTGCGACCTGCCCTGCGACCTGGACTGCGGCATCCTGGATGCCTGCTGCGAG
  TCCGCGGACTGCCTGGAGATCTGCATGGAGTGCTGTGGGCTCTGCTTCTCCTCCTGAGCC
  TCTGTCGGGGGCTAAGCCAGCCTGGCGCCCCTGCAGATTCCAGCAGGGTCCCTCTGAGTG
  GGGCCAGGCCCAGGACTGTCACACAAGGCTTGAGAAGCCCCCTCTCCCTGGTCCTCTCCT
  ACCCACCCATGTCCTCTCAGAACCCCAGCCTTGAAAATAGTGGGGGGCACTCAGAGGGGC
  CACCTCCTCAGCCGTGGGTGGTGGGCCCATGGCAGAGAAGCCTGAACTCTTTACTGGGTT
  ACCAGGTTCATACATTGCTGAGGACCTGACAGGACAACCTAGGGGCAGGGCTGGGGTGGG
  GACCGCAGGGGGCAGCCAGGGCTGGGGAACACTGTGAAAGTTACTTGGGGAGGGTGGGCC
  GGTGGGGCCGTAGCTCTCTACCTCTCCCTGCTCCTGGTGCCTGCCTCTCTCCTCCACCCC
  AGGCTTAGAGGACAGAAAAATGTGAAGAGACGCCCCACCCACCCTCAGCCAGCCCTCTCC
  AGTCTCCTTTCCTAGGCTTTTTTGGGGGCCTAACCCACGCAGTGACCCCAGAGGGCAGGG
  CTAGGCGAGAGCCTGGGGTGGGGCGGGAGGGGGAACAGTATGGAAAAGACTGGAAGGGGA
  AAGGAAGGGAAGGGAGGGAGGGTCTGTTCTATCTGTTGCTGTAAATAAAGATATTTGTCC
  ATCTCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC007836 unedited
  GGCGCCAGAATTGTATACGACTTATATAGGCGGCACGCGAATTCGCACGAGGAGAAAGCG
  CGAGTGAGAGGGGAAGGGGCGCCAGGCGAGCACCCGGGAGCCAGCGGGACCTGGGCAGGG
  GCGCCCGGAGCAGGCGCGCATGGCGGGCCCCGCGCGGGGATCCGGCTGGAAGAGAGCGTA
  GCACGGCTCGCACGAGTCCGGGGCCGATGTACCAGGTGAGCGGCCAGCGCCCCTCTGGCT
  GCGACGCGCCCTATGGAGCCCCCAGCGCAGCCCCGGGCCCAGCCCAGACCCTATCCCTCC
  TTCCTGGGCTGGAGGTAGTAACAGGATCCACTCACCCTGCGGAGGCAGCACCAGAGGAGG
  GCTCCCTGGAGGAGGCGGCAACCCCCATGCCCCAAGGCAATGGCCCTGGCATCCCCCAGG
  GCCTGGACAGCACTGACCTCGACGTCCCCACAGAAGCTGTGACATGCCAGCCTCAGGGGA
  ACCCCTTGGGCTGCACCCCACTTCTGCCGAATGACTCTGGCCACCCCTCAGAGCTGGGCG
  GCACCAGACGGGCGGGGAATGGTGCCCTGGGTGGCCCCAAGGCCCACCGGAAGTTGCAGA
  CACACCCATCTCTCGCCAGCCAGGGCAGCAAGAAGAGTAAGAGCAGCAGCAAATCCACCA
  CCTCCCAGATCCCCCTCCAGGCACAGGAAGACTGCTGTGTCCACTGCATCCTGTCCTGCC
  TGTTCTGCGAGTTCCTGACGCTGTGCAACATCGTCCTGGACTGCGCCACCTGTGGCTCCT
  GCAGCTCGGAGGACTCGTGCCTCTGCTGCTGCTGCTGTGGCTCTGGCGAGTGTGCCGACT
  GCGACCTGCCCTGCGACCTGGACTGCGGCATCCTGGATGCCTGCTGCGAGTCCGCGGCT
  
• Restriction Sites: Please inquire
• ACCN: BC007836
• Insert Size: 1592 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC007836.2, AAH07836.1
• RefSeq Size: 1583 bp
• Locus ID: 4188
• Cytogenetics: 6p21.1
• Protein Families: Transcription Factors
• Gene Summary: 'This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008]'