CHRM1 (BC007740) Human Untagged Clone

CAT#: SC123739

CHRM1 (untagged)-Human cholinergic receptor, muscarinic 1 (cDNA clone MGC:13136 IMAGE:4128514), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRM1
• Synonyms: cholinergic receptor, muscarinic 1; HM1; M1; MGC30125; muscarinic acetylcholine receptor M1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC007740 edited
  GGCAGTGGGGTTGAGGACCCTACAGACCCCTCTTCAGCCCCGTGGTGATGACTTTCCCCT
  GAGGAAGCCCTGTAGCGTGCCTGGAGGAAGGGGCTCTCCAACCCCAGCCCCACCTAGCCA
  CCATGAACACTTCAGCCCCACCTGCTGTCAGCCCCAACATCACCGTCCTGGCACCAGGAA
  AGGGTCCCTGGCAAGTGGCCTTCATTGGGATCACCACGGGCCTCCTGTCGCTAGCCACAG
  TGACAGGCAACCTGCTGGTACTCATCTCTTTCAAGGTCAACACGGAGCTCAAGACAGTCA
  ATAACTACTTCCTGCTGAGCCTGGCCTGTGCTGACCTCATCATCGGTACCTTCTCCATGA
  ACCTCTATACCACGTACCTGCTCATGGGCCACTGGGCTCTGGGCACGCTGGCTTGTGACC
  TCTGGCTGGCCCTGGACTATGTGGCCAGCAATGCCTCCGTCATGAATCTGCTGCTCATCA
  GCTTTGACCGCTACTTCTCCGTGACTCGGCCCCTGAGCTACCGTGCCAAGCGCACACCCC
  GCCGGGCAGCTCTGATGATCGGCCTGGCCTGGCTGGTTTCCTTTGTGCTCTGGGCCCCAG
  CCATCCTCTTCTGGCAGTACCTGGTAGGGGAGCGGACAGTGCTAGCTGGGCAGTGCTACA
  TCCAGTTCCTCTCCCAGCCCATCATCACCTTTGGCACAGCCATGGCTGCCTTCTACCTCC
  CTGTCACAGTCATGTGCACGCTCTACTGGCGCATCTACCGGGAGACAGAGAACCGAGCAC
  GGGAGCTGGCAGCCCTTCAGGGCTCCGAGACGCCAGGCAAAGGGGGTGGCAGCAGCAGCA
  GCTCAGAGAGGTCTCAGCCAGGGGCTGAGGGCTCACCAGAGACTCCTCCAGGCCGCTGCT
  GTCGCTGCTGCCGGGCCCCCAGGCTGCTGCAGGCCTACAGCTGGAAGGAAGAAGAGGAAG
  AGGACGAAGGCTCCATGGAGTCCCTCACATCCTCAGAGGGAGAGGAGCCTGGCTCCGAAG
  TGGTGATCAAGATGCCAATGGTGGACCCCGAGGCACAGGCCCCCACCAAGCAGCCCCCAC
  GGAGCTCCCCAAATACAGTCAAGAGGCCGACTAAGAAAGGGCGTGATCGAGCTGGCAAGG
  GCCAGAAGCCCCGTGGAAAGGAGCAGCTGGCCAAGCGGAAGACCTTCTCGCTGGTCAAGG
  AGAAGAAGGCGGCTCGGACCCTGAGTGCCATCCTCCTGGCCTTCATCCTCACCTGGACAC
  CGTACAACATCATGGTGCTGGTGTCCACCTTCTGCAAGGACTGTGTTCCCGAGACCCTGT
  GGGAGCTGGGCTACTGGCTGTGCTACGTCAACAGCACCATCAACCCCATGTGCTACGCAC
  TCTGCAACAAAGCCTTCCGGGACACCTTTCGCCTGCTGCTGCTTTGCCGCTGGGACAAGA
  GACGCTGGCGCAAGATCCCCAAGCGCCCTGGCTCCGTGCACCGCACTCCCTCCCGCCAAT
  GCTGATAGTCCCCTCTCCTGCATCCCTCCACCCCAGTCCCCGGGAAAGGCCGGTGGGAAG
  AGGGCAGGGGCTGCATCCTCAGCCCCAGGGCCCTGCTCAGGCCTCACCTGGCTTCCCAGG
  ACCCTGGGTCACCTTCCTGGGCAGCCCAGAGAGACCCTGCCAACTTTCCAGACTTCGCTA
  TTCCCAGGCAGGGAGGGAAACCCGGGGAACTGGTTTTTCTGTTCCCTGCTGGGTGGGAAT
  GCGCTCTTCACCAGGAAGAAGGCCCGGGAGGAGGATCCGGGCTTTGGACTCCTTGTTTGC
  CTTTAGGCAGGAAGTCAGGAGCCAGCAGGGCGGGCCAGGAGAAAGAAGGCTTAACATTAA
  GTATTCCTTGGCCCAGCAGCGGCCCAGATTGCGGTGTGAGATGGTGCCCCCTGGGGGGCA
  CAGCCAGAAACTGAACTGGCCGCTGGGAGAAAAGCCAGATGACAGGGAGCTGGGGAATCC
  CCTCGCTTCATAGGCAGAGCCCGCCCACCTGGGCCCTAGGCATACTCTCCAGGATTGTCC
  ACAAATGTCCTCAGAGGGTCCCTAGGTGGGTCAACTCCAAGGCAAATGTCCAAGCATCAG
  CAAGACAATGACACTGGAAGGGTCCGGCTTGGCTAGTCACATATCAAGTCCCGAGGCAGC
  AACAGGACCAGGAGCCAGGTGTCCTGACTGTCCTACAATATCATTTTCCTGGGAGTGGGA
  GTCAAGTGTGCCTGCTATCCAGCCGCAAATCCATACCCCCTGCCCCAGAGAAGCCTCAGT
  CCCTCCCTCCTGGCTCACAGCCACCACCTGGATGGATCTGCTCCATGCAGATCTAGCCAG
  GCCTCCCGCATGCTGCCTGCCTCCGGCCCTGCCCCACACAGGCCTGGCCCAGCCAGCAGG
  TTCTCTCCTGTGAGCTCCCCAATCCAACCCATGCATGGCCTCCCAGCCACCCGGATCTCC
  AGGCCCAGCCTGGCCCCAAATGTTCTTTCCTTTCATCCTCAGCAAGTGCTGAGTCTGTGA
  ATAAAGCCACATAACCAGCGGGCAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC007740 unedited
  NGAGTCATCTTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCAGTG
  GGGTTGAGGACCCTACATACCCCTCTTCAGCCCCGTGGTGATGACTTTCCCCTGAGGAAG
  CCCTGTAGCGTGCCTGGAGGAAGGGGCTCTCCAACCCCAGCCCCACCTAGCCACCATGAA
  CACTTCAGCCCCACCTGCTGTCAGCCCCAACATCACCGTCCTGGCACCAGGAAAGGGTCC
  CTGGCAAGTGGCCTTCATTGGGATCACCACGGGCCTCCTGTCGCTAGCCACAGTGACAGG
  CAACCTGCTGGTACTCATCTCTTTCAAGGTCAACACGGAGCTCAAGACAGTCAATAACTA
  CTTCCTGCTGAGCCTGGCCTGTGCTGACCTCATCATCGGTACCTTCTCCATGAACCTCTA
  TACCACGTACCTGCTCATGGGCCACTGGGCTCTGGGCACGCTGGCTTGTGACCTCTGGCT
  GGCCCTGGACTATGTGGCCAGCAATGCCTCCGTCATGAATCTGCTGCTCATCAGCTTTGA
  CCGCTACTTCTCCGTGACTCGGCCCCTGAGCTACCGTGCCAAGCGCACACCCCGCCGGGC
  AGCTCTGATGATCGGCCTGGCCTGGCTGGTTTCCTTTGTGCTCTGGGCCCCAGCCATCCT
  CTTCTGGCAGTACCTGGTAGGGGAGCGGACAGTGCTAGCTGGGCAGTGCTACATCCAGTT
  CCTCTCCCAGCCCATCATCACCTTTGGCACAGCCATGGCTGCCTTCTACCTCCCTGTCAC
  AGTCATGTGCACGCTCTACTGGCGCATCTACCGGGAGACAGAGAACCGAGCACGGGAGCT
  GGCAGCCCTTCAGGGCTCCGAGACGCCAGGCTAGGGGGTGGCAGCAGCACG
  
• Restriction Sites: Please inquire
• ACCN: BC007740
• Insert Size: 2574 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC007740.2, AAH07740.1
• RefSeq Size: 2565 bp
• RefSeq ORF: 1380 bp
• Locus ID: 1128
• Cytogenetics: 11q12.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Gene Summary: 'The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008]'