GALE (BC001273) Human Untagged Clone

CAT#: SC123789

GALE (untagged)-Human UDP-galactose-4-epimerase, transcript variant 2 (cDNA clone MGC:5083 IMAGE:3459004), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALE
• Synonyms: FLJ95174; FLJ97302; galactose-4-epimerase, UDP-; galactowaldenase; OTTHUMP00000002991; OTTHUMP00000044857; SDR1E1; short chain dehydrogenase/reductase family 1E, member 1; UDP-galactose-4-epimerase; UDP galactose-4'-epimerase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC001273 edited
  GGGAACCCGAGCAGGACTCTCCAGTCCTCAGTCACCTTGGACAAAGAAGTGTGGATCCTC
  AGATTCCATCTTTTCCAACTCCAAGGTGCCATGGCAGAGAAGGTGCTGGTAACAGGTGGG
  GCTGGCTACATTGGCAGCCACACGGTGCTGGAGCTGCTGGAGGCTGGCTACTTGCCTGTG
  GTCATCGATAACTTCCATAATGCCTTCCGTGGAGGGGGCTCCCTGCCTGAGAGCCTGCGG
  CGGGTCCAGGAGCTGACAGGCCGCTCTGTGGAGTTTGAGGAGATGGACATTTTGGACCAG
  GGAGCCCTACAGCGTCTCTTCAAAAAGTACAGCTTTATGGCGGTCATCCACTTTGCGGGG
  CTCAAGGCCGTGGGCGAGTCGGTGCAGAAGCCTCTGGATTATTACAGAGTTAACCTGACC
  GGGACCATCCAGCTTCTGGAGATCATGAAGGCCCACGGGGTGAAGAACCTGGTGTTCAGC
  AGCTCAGCCACTGTGTACGGGAACCCCCAGTACCTGCCCCTTGATGAGGCCCACCCCACG
  GGTGGTTGTACCAACCCTTACGGCAAGTCCAAGTTCTTCATCGAGGAAATGATCCGGGAC
  CTGTGCCAGGCAGACAAGACTTGGAACGCAGTGCTGCTGCGCTATTTCAACCCCACAGGT
  GCCCATGCCTCTGGCTGCATTGGTGAGGATCCCCAGGGCATACCCAACAACCTCATGCCT
  TATGTCTCCCAGGTGGCGATCGGGCGACGGGAGGCCCTGAATGTCTTTGGCAATGACTAT
  GACACAGAGGATGGCACAGGTGTCCGGGATTACATCCATGTCGTGGATCTGGCCAAGGGC
  CACATTGCAGCCTTAAGGAAGCTGAAAGAACAGTGTGGCTGCCGGATCTACAACCTGGGC
  ACGGGCACAGGCTATTCAGTGCTGCAGATGGTCCAGGCTATGGAGAAGGCCTCTGGGAAG
  AAGATCCCGTACAAGGTGGTGGCACGGCGGGAAGGTGATGTGGCAGCCTGTTACGCCAAC
  CCCAGCCTGGCCCAAGAGGAGCTGGGGTGGACAGCAGCCTTAGGGCTGGACAGGATGTGT
  GAGGATCTCTGGCGCTGGCAGAAGCAGAATCCTTCAGGCTTTGGCACGCAAGCCTGAGGA
  CCCTCCCCTACCAAGGACCAGGAAAAGCAGCAGCTGCCTGCTCTCCAGCCTCTGGCAGGA
  ACTCAGGGCCCTGGAGCTGCTGGGGCCAAGCCAAGGCCTCCCCTACCTCAAACCCCAGCT
  GGGCCCGCTTAGCCCACCAGGCATGAGGCCAAGGGCTCCACTGACCAGGAGGCCGAGGTC
  TCTAACTCTTATCTTCCACAGGGTCCAAGAGTTCATCAGGACCCCCAAGAGTGAGTGAGG
  GGGCAAGGCTCTGGCACAAAACCTCCTCCTCCCAGGCACTCATTTATATTGCTCTGAAAG
  AGCTTTCCAAAGTATTTAAAAATAAAAACAAGTTTTCTTACACTGGGAAAAAAAAAAAAA
  AAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC001273 unedited
  AAACTGATTTGTAAACCATTATATAGGCGGCCGCGAATCCCGGGNAATCGTCGACCCACG
  CGTCCGGGGAACCCGAGCAGGACTCTCCAGTCCTCAGTCACCTTGGACAAAGAAGTGTGG
  ATCCTCAGATTCCATCTTTTCCAACTCCAAGGTGCCATGGCAGAGAAGGTGCTGGTAACA
  GGTGGGGCTGGCTACATTGGCAGCCACACGGTGCTGGAGCTGCTGGAGGCTGGCTACTTG
  CCTGTGGTCATCGATAACTTCCATAATGCCTTCCGTGGAGGGGGCTCCCTGCCTGAGAGC
  CTGCGGCGGGTCCAGGAGCTGACAGGCCGCTCTGTGGAGTTTGAGGAGATGGACATTTTG
  GACCAGGGAGCCCTACAGCGTCTCTTCAAAAAGTACAGCTTTATGGCGGTCATCCACTTT
  GCGGGGCTCAAGGCCGTGGGCGAGTCGGTGCAGAAGCCTCTGGATTATTACAGAGTTAAC
  CTGACCGGGACCATCCAGCTTCTGGAGATCATGAAGGCCCACGGGGTGAAGAACCTGGTG
  TTCAGCAGCTCAGCCACTGTGTACGGGAACCCCCAGTACCTGCCCCTTGATGAGGCCCAC
  CCCACGGGTGGTTGTACCAACCCTTACGGCAAGTCCAAGTTCTTCATCGAGGAAATGATC
  CGGGACCTGTGCCAGGCAGACAAGACTTGGAACGCAGTGCTGCTGCGCTATTTCAACCCC
  ACAGGTGCCCATGCCTCTGGCTGCATTGGTGAGGATCCCCAGGGCATACCCAACAACCTC
  ATGCCTTATGTCTCCCCAGTGGCGATCGGGCGACGGGAGGCCCTGAATGTCTTTTGCATG
  ACTATGACACAGAGGATGGCACAGGTGTCCGGGATTACATCCATGTCGTGGATCTTGCCC
  AGGGCACATTGCAGCCTTAG
  
• Restriction Sites: Please inquire
• ACCN: BC001273
• Insert Size: 1503 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC001273.1, AAH01273.1
• RefSeq Size: 1503 bp
• Locus ID: 2582
• Cytogenetics: 1p36.11
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and cognitive disability, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Jul 2008]'