TRPM8 (BC001135) Human Untagged Clone

CAT#: SC123793

TRPM8 (untagged)-Homo sapiens, clone MGC:2849 IMAGE:2967357, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPM8
• Synonyms: LTRPC6; MGC2849; OTTHUMP00000065206; short form of the TRPM8 cationic channel; transient receptor potential cation channel, subfamily M, member 8; transient receptor potential subfamily M member 8; TRPP8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC001135 edited
  GGCACGAGGCTGCCTTTCTCCACCAGAGACTCTTCCTCAGGGAGGACTTGGTGAATTTTA
  TTCAAGCAAATTTTAAGAAACGAGAATGTGTCTTCTTTACCAAAGATTCCAAGGCCACGC
  TCAATGAAATCCTTCCTTCCTGTCCACACCATCGTGCTTATCAGGGAGAATGTGTGCAAG
  TGTGGCTATGCCCAGAGCCAGCACATGGAAGGCACCCAGATCAACCAAAGTGAGAAATGG
  AACTACAAGAAACACACCAAGGAATTTCCTACCGACGCCTTTGGGGATATTCAGTTTGAG
  ACACTGGGGAAGAAAGGGAAGTATATACGTCTGTCCTGCGACACGGACGCGGAAATCCTT
  TACGAGCTGCTGACCCAGCACTGGCACCTGAAAACACCCAACCTGGTCATTTCTGTGACC
  GGGGGCGCCAAGAACTTCGCCCTGAAGCCGCGCATGCGCAAGATCTTCAGCCGGCTCATC
  TACATCGCGCAGTCCAAAGGTGCTTGGATTCTCACGGGAGGCACCCATTATGGCCTGATG
  AAGTACATCGGGGAGGTGGTGAGAGATAACACCATCAGCAGGAGTTCAGAGGAGAATATT
  GTGGCCATTGGCATAGCAGCTTGGGGCATGGTCTCCAACCGGGACACCCTCATCAGGAAT
  TGCGATGCTGAGGTACCGGTGGGACAGGAGGAGGTCTGCTAGGTCACATGGAAGAAAGAC
  CATGGCATGGGCCTGTGGCCTGAACCCTGGGGCTCTGTGATGGAGCCAGCCAGATCATGG
  GGAAGTCTGCCTTTCAAGGAGTGCCTTTGGGACCTTAAAGGAATTGAAAACAAGGATGAC
  GTACCTAATTAACTGCTGGGAAAGAGTTAACAATGAATGTTTTGTTCATTAAAATGTGTT
  CTCAGCAATCTCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC001135 unedited
  GAGACCTGGATTTGTAATACGACTTTATATAGGCGGCCGCGCAATTCGGCACGAGGCTTG
  CCTTTCTCCACCAAATACTCTTCCTCAGGGAGGACTTGGTGAATTTTATTCAAGCAAATT
  TTAAGAAACGAGAATGTGTCTTCTTTACCAAAGATTCCAAGGCCACGCTCAATGAAATCC
  TTCCTTCCTGTCCACACCATCGTGCTTATCAGGGAGAATGTGTGCAAGTGTGGCTATGCC
  CAGAGCCAGCACATGGAAGGCACCCAGATCAACCAAAGTGAGAAATGGAACTACAAGAAA
  CACACCAAGGAATTTCCTACCGACGCCTTTGGGGATATTCAGTTTGAGACACTGGGGAAG
  AAAGGGAAGTATATACGTCTGTCCTGCGACACGGACGCGGAAATCCTTTACGAGCTGCTG
  ACCCAGCACTGGCACCTGAAAACACCCAACCTGGTCATTTCTGTGACCGGGGGCGCCAAG
  AACTTCGCCCTGAAGCCGCGCATGCGCAAGATCTTCAGCCGGCTCATCTACATCGCGCAG
  TCCAAAGGTGCTTGGATTCTCACGGGAGGCACCCATTATGGCCTGATGAAGTACATCGGG
  GAGGTGGTGAGAGATAACACCATCAGCAGGAGTTCAGAGGAGAATATTGTGGCCATTGGC
  ATAGCAGCTTGGGGCATGGTCTCCAACCGGGACACCCTCATCANGAATTGCGATGCTGAN
  GTACCNNGTGGGACAGAGGAGGTCTGCTAGGTCACATGGAAGATAGACCATGGCATGGGC
  CTGTGGCCTGAACCCTGGGGCTCTGTGATGGAGCCAGCCAGATCATGGGGAAGTCTGCCT
  TTCAAGGAGTAGCCTTTGGGACCTTAAAGGATTTTGAAACAAGGATGACGTAACTAATTA
  ACTGCTGGGGAAAGATT
  
• Restriction Sites: Please inquire
• ACCN: BC001135
• ORF Size: 579 bp
• Insert Size: 930
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: BC001135.1, AAH01135.1
• RefSeq Size: 930
• RefSeq ORF: 579
• Locus ID: 79054
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Plays a role in prostate cancer cell migration (PubMed:25559186). Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel. [UniProtKB/Swiss-Prot Function]