PDE6 beta (PDE6B) (BC000249) Human Untagged Clone

CAT#: SC123804

PDE6B (untagged)-Human phosphodiesterase 6B, cGMP-specific, rod, beta (congenital stationary night blindness 3, autosomal dominant) (cDNA clone MGC:1689 IMAGE:3356353), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE6B
• Synonyms: congenital stationary night blindness 3, autosomal dominant; CSNB3; PDEB; phosphodiesterase 6B, cGMP-specific, rod, beta; rd1; RP40
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC000249 edited
  TGCGTGCCTGGAGCAGCAGCGTCTCCAGGGACAGGCAGCCACCATGAGCCTCAGTGAGGA
  GCAGGCCCGGAGCTTTCTGGACCAGAACCCCGATTTTGCCCGCCAGTACTTTGGGAAGAA
  ACTGAGCCCTGAGAATGTGGCCGCGGCCTGCGAGGACGGGTGCCCGCCGGACTGCGACAG
  CCTCCGGGACCTCTGCCAGGTGGAGGAGAGCACGGCGCTGCTGGAGCTGGTGCAGGATAT
  GCAGGAGAGCATCAACATGGAGCGCGTGGTCTTCAAGGTCCTGCGGCGCCTCTGCACCCT
  CCTGCAGGCCGACCGCTGCAGCCTCTTCATGTACCGCCAGCGCAACGGCGTGGCCGAGCT
  GGCCACCAGGCTTTTCAGCGTGCAGCCGGACAGCGTCCTGGAGGACTGCCTGGTGCCCCC
  CGACTCCGAGATCGTCTTCCCACTGGACATCGGGGTCGTGGGCCACGTGGCTCAGACCAA
  AAAGATGGTGAACGTCGAGGACGTGGCCGAGTGCCCTCACTTCAGCTCATTTGCTGACGA
  GCTCACTGACTACAAGACAAAGAATATGCTGGCCACACCCATCATGAATGGCAAAGACGT
  CGTGGCGGTGATCATGGCAGTGAACAAGCTCAACGGCCCATTCTTCACCAGCGAAGACGA
  AGATGTGTTCTTGAAGTACCTGAATTTTGCCACGTTGTACCTGAAGATCTATCACCTGAG
  CTACCTCCACAACTGCGAGACGCGCCGCGGCCAGGTGCTGCTGTGGTCGGCCAACAAGGT
  GTTTGAGGAGCTGACGGACATCGAGAGGCAGTTCCACAAGGCCTTCTACACGGTGCGGGC
  CTACCTCAACTGCGAGCGGTACTCCGTGGGCCTCCTGGACATGACCAAGGAGAAGGAATT
  TTTTGACGTGTGGTCTGTGCTGATGGGAGAGTCCCAGCCGTACTCGGGCCCACGCACGCC
  TGATGGCCGGGAAATTGTCTTCTACAAAGTGATCGACTACATCCTCCACGGCAAGGAGGA
  GATCAAGGTCATTCCCACACCCTCAGCCGATCACTGGGCCCTGGCCAGCGGCCTTCCAAG
  CTACGTGGCAGAAAGCGGCTTTATTTGTAACATCATGAATGCTTCCGCTGACGAAATGTT
  CAAATTTCAGGAAGGGGCCCTGGACGACTCCGGGTGGCTCATCAAGAATGTGCTGTCCAT
  GCCCATCGTCAACAAGAAGGAGGAGATTGTGGGAGTCGCCACATTTTACAACAGGAAAGA
  CGGGAAGCCCTTTGACGAACAGGACGAGGTTCTCATGGAGTCCCTGACACAGTTCCTGGG
  CTGGTCAGTGATGAACACCGACACCTACGACAAGATGAACAAGCTGGAGAACCGCAAGGA
  CATCGCACAGGACATGGTCCTTTACCACGTGAAGTGCGACAGGGACGAGATCCAGCTCAT
  CCTGCCAACCAGAGCGCGCCTGGGGAAGGAGCCTGCTGACTGCGATGAGGACGAGCTGGG
  CGAAATCCTGAAGGAGGAGCTGCCAGGGCCCACCACATTTGACATCTACGAATTCCACTT
  CTCTGACCTGGAGTGCACCGAACTGGACCTGGTCAAATGTGGCATCCAGATGTACTACGA
  GCTGGGCGTGGTCCGAAAGTTCCAGATCCCCCAGGAGGTCCTGGTGCGGTTCCTGTTCTC
  CATCAGCAAAGGGTACCGGAGAATCACCTACCACAACTGGCGCCACGGCTTCAACGTGGC
  CCAGACGATGTTCACGCTGCTCATGACCGGCAAACTGAAGAGCTACTACACGGACCTGGA
  GGCCTTCGCCATGGTGACAGCCGGCCTGTGCCATGACATCGACCACCGCGGCACCAACAA
  CCTGTACCAGATGAAGTCCCAGAACCCCTTGGCTAAGCTCCACGGCTCCTCGATTTTGGA
  GCGGCACCACCTGGAGTTTGGGAAGTTCCTGCTCTCGGAGGAGACCCTGAACATCTACCA
  GAACCTGAACCGGCGGCAGCACGATCACGTGATCCACCTGATGGACATCGCCATCATCGC
  CACGGACCTGGCCCTGTACTTCAAGAAGAGAGCGATGTTTCAGAAGATCGTGGATGAGTC
  CAAGAACTACCAGGACAAGAAGAGCTGGGTGGAGTACCTGTCCCTGGAGACGACCCGGAA
  GGAGATCGTCATGGCCATGATGATGACAGCCTGCGACCTGTCTGCCATCACCAAGCCCTG
  GGAAGTCCAGAGCAAGGTCGCACTTCTCGTGGCTGCTGAGTTCTGGGAGCAAGGTGACTT
  GGAAAGGACAGTCTTGGATCAGCAGCCCATTCCTATGATGGACCGGAACAAGGCGGCCGA
  GCTCCCCAAGCTGCAAGTGGGCTTCATCGACTTCGTGTGCACATTCGTGTACAAGGAGTT
  CTCTCGTTTCCACGAAGAGATCCTGCCCATGTTCGACCGACTGCAGAACAATAGGAAAGA
  GTGGAAGGCGCTGGCTGATGAGTATGAGGCCAAAGTGAAGGCTCTGGAGGAGAAGGAGGA
  GGAGGAGAGGGTGGCAGCCAAGAAAGGCACAGAAATTTGCAATGGCGGCCCAGCACCCAA
  GTCTTCAACCTGCTGTATCCTGTGAGCACTGGTCCCATGGGGACCCTATGGCTCCCTCAA
  TCTTCACCCACTAGGATTTGGGTTCTGCCTGTGGCTATTTGCTACAAGAGGTTAGGAAGC
  CCAAGAAAATGACTGAAGATCATTCTGGATATTTTAATTTTTTTTTTTTTTTTTTTTTGA
  GATGGAGTCTTGCTCTGTCACCCAGGCTGGAGTGCCGTGGCACGATCTCAGCTCACTGCA
  ACCTCCACCTCCCAGGTTCAAGCGATTCTCGTGCCTCAGCCTCCTGAGTAGCTGGGACTA
  CAGGCGCCCACCACCACACATGGCTAATTTTTGTATTTTCAGTACAGATGGGGTTTCACC
  ATATTGGGCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCACCCGCCTCAGCTTCCTG
  AAGTGCTGGGATTACAGGCATGAGCCACCACGCCCAGCCTGTTTTTATAAACTGAAGCCA
  ACTGTGAATAAACTGTAGCCTACAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC000249 unedited
  AAAAACCGTTCANCATTTGTAATACGACTTACTATAGGCGGCACGCGAATTCGCACGAGG
  TGCGTGCCTGGAGCAGCAGCGTCTCCAGGGACAGGCAGCCACCATGAGCCTCAGTGAGGA
  GCAGGCCCGGAGCTTTCTGGACCAGAACCCCGATTTTGCCCGCCAGTACTTTGGGAAGAA
  ACTGAGCCCTGAGAATGTGGCCGCGGCCTGCGAGGACGGGTGCCCGCCGGACTGCGACAG
  CCTCCGGGACCTCTGCCAGGTGGAGGAGAGCACGGCGCTGCTGGAGCTGGTGCAGGATAT
  GCAGGAGAGCATCAACATGGAGCGCGTGGTCTTCAAGGTCCTGCGGCGCCTCTGCACCCT
  CCTGCAGGCCGACCGCTGCAGCCTCTTCATGTACCGCCAGCGCAACGGCGTGGCCGAGCT
  GGCCACCAGGCTTTTCAGCGTGCAGCCGGACAGCGTCCTGGAGGACTGCCTGGTGCCCCC
  CGACTCCGAGATCGTCTTCCCACTGGACATCGGGGTCGTGGGCCACGTGGCTCAGACCAA
  AAAGATGGTGAACGTCGAGGACGTGGCCGAGTGCCCTCACTTCAGCTCATTTGCTGACGA
  GCTCACTGACTACAAGACAAAGAATATGCTGGCCACACCCATCATGAATGGCAAAGACGT
  CGTGGCGGTGATCATGGCAGTGAACAAGCTCAACGGCCCATTCTTCACCAGCGAAGACGA
  AGATGTGTTCTTGAAGTACCTGAATTTTGCCACGTTGTACCTGAAGATCTATCACCTGAG
  CTACCTCCACAACTGCGAGACGCGCCGCGGCCCAGTGCTGCTGTGGTCGGCCAACAAGGT
  GTTTTGAGAGCTGACGGACATCGAGAGGCAGTT
  
• Restriction Sites: Please inquire
• ACCN: BC000249
• Insert Size: 3109 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC000249.1, AAH00249.1
• RefSeq Size: 3109 bp
• Locus ID: 5158
• Cytogenetics: 4p16.3
• Protein Families: Druggable Genome
• Gene Summary: 'Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]'