PPAR alpha (PPARA) (NM_032644) Human Untagged Clone

CAT#: SC123806

PPARA (untagged)-Human peroxisome proliferative activated receptor, alpha (PPARA), transcript variant 7

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPARA
• Synonyms: PPAR, NR1C1, hPPAR, MGC2237, MGC2452
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032644 edited
  GAAGTTCAAGATCAAAGTGCCAGCAGATTCAGTGTCATGTGAGGACGTGCTTCCTGCTTC
  ATAGATAAGAGTGCCACACCCCCCTGTCCCAGTGCACTGGGGCTGTGGATCCCTTCAAAG
  CTGAGATTGCCTGTCTGTGGTCTCCAGCGTTAAGCACAGTCATTAGCTCAGCTTGGAGCT
  CGGCGGCACAACCAGCACCATCTGGTCGCGATGGTGGACACGGAAAGCCCACTCTGCCCC
  CTCTCCCCACTCGAGGCCGGCGATCTAGAGAGCCCGTTATCTGAAGAGTTCCTGCAAGAA
  ATGGGAAACATCCAAGAGATTTCGCAATCCATCGGCGAGGATAGTTCTGGAAGCTTTGGC
  TTTACGGAATACCAGTATTTAGGAAGCTGTCCTGGCTCAGATGGCTCGGTCATCACGGAC
  ACGCTTTCACCAGCTTCGAGCCCCTCCTCGGTGACTTATCCTGTGGTCCCCGGCAGCGTG
  GACGAGTCTCCCAGTGGAGCATTGAACATCGAATGTAGAATCTGCGGGGACAAGGCCTCA
  GGCTATCATTACGGAGTCCACGCGTGTGAAGGCTGCAAGGGCTTCTTTCGGCGAACGATT
  CGACTCAAGCTGGTGTATGACAAGTGCGACCGCAGCTGCAAGATCCAGAAAAAGAACAGA
  AACAAATGCCAGTATTGTCGATTTCACAAGTGCCTTTCTGTCGGGATGTCACACAACGCG
  ATTCGTTTTGGACGAATGCCAAGATCTGAGAAAGCAAAACTGAAAGCAGAAATTCTTACC
  TGTGAACATGACATAGAAGATTCTGAAACTGCAGATCTCAAATCTCTGGCCAAGAGAATC
  TACGAGGCCTACTTGAAGAACTTCAACATGAACAAGGTCAAAGCCCGGGTCATCCTCTCA
  GGAAAGGCCAGTAACAATCCAGTAGGTGTTTGCGGCTGTTCTGGGTTCTCTTGGCAACAT
  GGAACCAGTGTCGTAGAGGACGATTAAGGACACATGTGTTGAATGTTGAGAAAATTATAT
  TTATCCCACAGTTAAGCAAAGGACAGCGAAGATGGAAACAGTTCATTCTGAGACTCTGAG
  CTGTAGCTTAACAACAACTCCTTTCTTCTTGCTTGGAGCCACCTCAAAGCTCTTAGCAAC
  TAAGTTATTATACTGGCTATGTAATTAATACACTTAAAAAAAACCTTAATAGCTTACCAA
  GTACTAAGATGATTTCTTAGGAGCATTTTTTCTTAAATAGAGATAGGTTCTTGCTCTGTT
  GCCCAGGCTGGAATGCAGTGGTGCAATCATAGTTCACTGCAGCCTTGAACTCCTGGGCTC
  AAGCAATCCTCCTGCCTCAGCCTCCCAAGGAGCTGGGACTACAGGTGTGCACCACCACAC
  CTGGCTATGTTTGATGTTGTTGTTGTTTTGTTTTGTTTTTGTTTTTTGGTAGAGATGAGA
  TGTTTCCCAGGCTGGTCTCAAACTCCTGGCCTCAAGTGATCTTCCCACCTCGGCCTCCCA
  AAGCACTGGCATTACAGGTGTGAGTCATGGCACCCAGCATTAACTGGATTTAAAAAAAAA
  AAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032644 unedited
  NAAAGTACAGCATTTGTNATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGNAA
  GTTCAATATCAAAGTGCCAGCAGATTCAGTGTCATGTGAGGACGTGCTTCCTGCTTCATA
  GATAAGAGTGCCACACCCCCCTGTCCCAGTGCACTGGGGCTGTGGATCCCTTCAAAGCTG
  AGATTGCCTGTCTGTGGTCTCCAGCGTTAAGCACAGTCATTAGCTCAGCTTGGAGCTCGG
  CGGCACAACCAGCACCATCTGGTCGCGATGGTGGACACGGAAAGCCCACTCTGCCCCCTC
  TCCCCACTCGAGGCCGGCGATCTAGAGAGCCCGTTATCTGAAGAGTTCCTGCAAGAAATG
  GGAAACATCCAAGAGATTTCGCAATCCATCGGCGAGGATAGTTCTGGAAGCTTTGGCTTT
  ACGGAATACCAGTATTTAGGAAGCTGTCCTGGCTCAGATGGCTCGGTCATCACGGACACG
  CTTTCACCAGCTTCGAGCCCCTCCTCGGTGACTTATCCTGTGGTCCCCGGCAGCGTGGAC
  GAGTCTCCCAGTGGAGCATTGAACATCGAATGTAGAATCTGCGGGGACAAGGCCTCAGGC
  TATCATTACGGAGTCCACGCGTGTGAAGGCTGCAAGGGCTTCTTTCGGCGAACGATTCGA
  CTCAAGCTGGTGTATGACAAGTGCGACCGCAGCTGCNAGATCCANGAAAAGAACAGAAAC
  AAATGCCAGTATTGTCGATTTCACAAGTGCCTTTCTGTCGGGATGTCACACAACGCGATT
  CGTTTTGGACGAATGCCAAGATCTGAGAAAGCANAACTGAAAGCAGAAATTCTTACCTGT
  GAACATGACATAGNAAGATTCTGAAACTGCAGATCTCAAATCTCTGGCCAAGAAATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032644 unedited
  CCATGGGGCCATTGGGNGATGTGCAACTTCCCAGGNCCAGNANAGCACTGGGGNAGGGTC
  ACAGGGCATGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTA
  GAGTCGAGTTTTTTTTTTTTTTTTTTTTAAATCCAGTTAATGCTGGGTGCCATGACTCAC
  ACCTGTAATGCCAGTGCTTTGGGAGGCCGAGGTGGGAAGATCACTTGAGGCCAGGAGTTT
  GAGACCAGCCTGGGAAACATCTTATCTCTACCAAAAAACAAAAACAAAACAAAACAACCA
  CAACATCAAACATAGCCAGGTGTGGTGGTGCACACCTGTAGTCCCAGCTCCTTGGGAGGC
  TGAGGCAGGAGGATTGCTTGAGCCCAGGAGTTCAAGGCTGCAGTGAACTATGATTGCACC
  ACTGCATTCCAGCCTGGGCAACAGAGCAAGAACCTATCTCTATTTAAGAAAAAATGCTCC
  TAAGAAATCATCTTAGTACTTGGTAAGCTATTAAGGTTTTTTTTAAGTGTATTAATTACA
  TAGCCAGTATAATAACTTAGTTGCTAAGAGCTTTGAGGTGGCTCCAAGCAAGAAGAAAGG
  AGTTGTTGTTAAGCTACAGCTCAGAGTCTCAGAATGAACTGTTTCCATCTTCGCTGTCCT
  TTGCTTAACTGTGGGATAAATATAATTTTCTCAACATTCAACACATGTGTCCTTAATCGT
  CCTCTACGACACTGGTTCCATGTTGCCAAGAGAACCCAGNAAAGCCGCAAACACCTACTG
  GATTGNTACTGGCCTTTCCTGAGAGGATGACCCGGGCTTTGACCTTGTTCATGTTGGAAT
  TCTTCAAGTAGGCCTCGTAGATTTCTTTGGCCAGAGATTTGAGATCTGCAGTTTAGAATT
  TTCTATG
  
• Restriction Sites: Please inquire
• ACCN: NM_032644
• Insert Size: 1580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032644.3, NP_116033.2
• RefSeq Size: 3370 bp
• RefSeq ORF: 777 bp
• Locus ID: 5465
• Cytogenetics: 22q13.31
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, PPAR signaling pathway
• Gene Summary: 'Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (7) differs in the 5' UTR and continues past a splice site in the 3' coding region which results in an early stop codon, compared to variant 6. The resulting isoform (2) has a distinct and shorter C-terminus when compared to isoform 1.