NGF (NM_002506) Human Untagged Clone

CAT#: SC123827

NGF (untagged)-Human nerve growth factor (beta polypeptide) (NGF)


  "NM_002506" in other vectors (6)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NGF
Synonyms Beta-NGF; HSAN5; NGFB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_002506 edited
CCACGCGTCCGCGGACGCGTGGGCGCTGGGAGCCGGAGGGGAGCGCAGCGAGTTTTGGCC
AGTGGTCGTGCAGTCCAAGGGGCTGGATGGCATGCTGGACCCAAGCTCAGCTCAGCGTCC
GGACCCAATAACAGTTTTACCAAGGGAGCAGCTTTCTATCCTGGCCACACTGAGGTGCAT
AGCGTAATGTCCATGTTGTTCTACACTCTGATCACAGCTTTTCTGATCGGCATACAGGCG
GAACCACACTCAGAGAGCAATGTCCCTGCAGGACACACCATCCCCCAAGTCCACTGGACT
AAACTTCAGCATTCCCTTGACACTGCCCTTCGCAGAGCCCGCAGCGCCCCGGCAGCGGCG
ATAGCTGCACGCGTGGCGGGGCAGACCCGCAACATTACTGTGGACCCCAGGCTGTTTAAA
AAGCGGCGACTCCGTTCACCCCGTGTGCTGTTTAGCACCCAGCCTCCCCGTGAAGCTGCA
GACACTCAGGATCTGGACTTCGAGGTCGGTGGTGCTGCCCCCTTCAACAGGACTCACAGG
AGCAAGCGGTCATCATCCCATCCCATCTTCCACAGGGGCGAATTCTCGGTGTGTGACAGT
GTCAGCGTGTGGGTTGGGGATAAGACCACCGCCACAGACATCAAGGGCAAGGAGGTGATG
GTGTTGGGAGAGGTGAACATTAACAACAGTGTATTCAAACAGTACTTTTTTGAGACCAAG
TGCCGGGACCCAAATCCCGTTGACAGCGGGTGCCGGGGCATTGACTCAAAGCACTGGAAC
TCATATTGTACCACGACTCACACCTTTGTCAAGGCGCTGACCATGGATGGCAAGCAGGCT
GCCTGGCGGTTTATCCGGATAGATACGGCCTGTGTGTGTGTGCTCAGCAGGAAGGCTGTG
AGAAGAGCCTGACCTGCCGACACGCTCCCTCCCCCTGCCCCTTCTACACTCTCCTGGGCC
CCTCCCTACCTCAACCTGTAAATTATTTTAAATTATAAGGACTGCATGGTAATTTATAGT
TTATACAGTTTTAAAGAATCATTATTTATTAAATTTTTGGAAGCAAAAAAAAAAAAAAA
>OriGene 5' read for NM_002506 unedited
GGGGAGTTCACATTTGTATACGACTCATATAGGCGGCCGACGATTCATATCTGGTACCGG
TCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCGGACGCGTGGGCGCTGGGAGCC
GGAGGGGAGCGCAGCGAGTTTTGGCCAGTGGTCGTGCAGTCCAAGGGGCTGGATGGCATG
CTGGACCCAAGCTCAGCTCAGCGTCCGGACCCAATAACAGTTTTACCAAGGGAGCAGCTT
TCTATCCTGGCCACACTGAGGTGCATAGCGTAATGTCCATGTTGTTCTACACTCTGATCA
CAGCTTTTCTGATCGGCATACAGGCGGAACCACACTCAGAGAGCAATGTCCCTGCAGGAC
ACACCATCCCCCAAGTCCACTGGACTAAACTTCAGCATTCCCTTGACACTGCCCTTCGCA
GAGCCCGCAGCGCCCCGGCAGCGGCGATAGCTGCACGCGTGGCGGGGCAGACCCGCAACA
TTACTGTGGACCCCAGGCTGTTTAAAAAGCGGCGACTCCGTTCACCCCGTGTGCTGTTTA
GCACCCAGCCTCCCCGTGAAGCTGCAGACACTCAGGATCTGGACTTCGAGGTCGGTGGTG
CTGCCCCCTTCAACAGGACTCACAGGAGCAAGCGGTCATCATCCCATCCCATCTTCCACA
GGGGCGAATTCTCGGTGTGTGACAGTGTCAGCGTGTGGGTTGGGGATAAGACCACCGCCA
CAGACATCAAGGGCAAGGAGGTGATGGTGTTGGGAGAGGTGAACATTAACAACAGTGTAT
TCAAACAGTACTTTTTTGAGACCAAGTGCCGGGACCCAAATCCCGTTGACAGCGGGTGCC
GGNGCATTGACTCANACACTGGNACTCATATTGTACCACGACTCACACCTTTNGTCAGGC
GCTGACCAG
>OriGene 3' read for NM_002506 unedited
CATAGTCATGGCATGGCACTTGCCAGGGCCAGGANAGCACTGGGNGAGGGTCACAGGGAT
GCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCCCTTTTTTTTTTTTTTT
GCTTCCAAAAATTTAATAAATAATGATTCTTTAAAACTGTATAAACTATAAATTACCATG
CAGTCCTTATAATTTAAAATAATTTACAGGTTGAGGTAGGGAGGGGCCCAGGAGAGTGTA
AAAGGGGCAGGGGGAGGGAGCGTGTCGGCAGGTCAGGCTCTTCTCACAGCCTTCCTGCTG
AGCACACACACACAGGCCGTATCTATCCGGATAAACCGCCAGGCAGCCTGCTTGCCATCC
ATGGTCAGCGCCTTGACAAAGGTGTGAGTCGTGGTACAATATGAGTTCCAGTGCTTTGAG
TCAATGCCCCGGCACCCGCTGTCAACGGGATTTGGGTCCCGGCACTTGGTCTCAAAAAAG
TACTGTTTGAATACACTGTTGTTAATGTTCACCTCTCCCAACACCATCACCTCCTTGCCC
TTGATGTCTGTGGCGGTGGTCTTATCCCCAACCCACACGCTGACACTGTCACACACCGAG
AATTCNCCCCTGTGGAAGATGGGATGGGATGATGACCGCTTGCTCCTGTGAGTCCTGTTG
AAGGGGGCAGCACCACCGACCTCGAAGTCCAGATCCTGAGTGTCTGCAGCTTCACGGGGA
GGCTGGGTGCTAAACAGCACACGGTGTGAACGGAGTCGCCGCTTTTTAAACAGCCTGGGG
TCCACAGTAATGGTTGCGGGTCTGCCCCGCCACGCGTGCAACTTTCGCCGCTGCCGGGGC
GCTCGGGCTCTTGAAAGGGCAGTGTCAAGGTATGCTTAAATTTAATCCGTGGACTGGGGG
AGTGC
Restriction Sites Please inquire     
ACCN NM_002506
Insert Size 1200 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002506.2, NP_002497.2
RefSeq Size 1052 bp
RefSeq ORF 726 bp
Locus ID 4803
Cytogenetics 1p13.2
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Apoptosis, MAPK signaling pathway, Neurotrophin signaling pathway
Gene Summary 'This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]'

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