HNF 4 alpha (HNF4A) (NM_000457) Human Untagged Clone

CAT#: SC123863

HNF4A (untagged)-Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2


  "NM_000457" in other vectors (6)

Reconstitution Protocol

USD 790.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HNF4A
Synonyms FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_000457 edited
AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCG
TCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGAGAGGGCA
CTGGGAGGAGGCAGTGGGAGGGCGGAGGGCGGGGGCCTTCGGGGTGGGCGCCCAGGGTAG
GGCAGGTGGCCGCGGCGTGGAGGCAGGGAGAATGCGACTCTCCAAAACCCTCGTCGACAT
GGACATGGCCGACTACAGTGCTGCACTGGACCCAGCCTACACCACCCTGGAATTTGAGAA
TGTGCAGGTGTTGACGATGGGCAATGACACGTCCCCATCAGAAGGCACCAACCTCAACGC
GCCCAACAGCCTGGGTGTCAGCGCCCTGTGTGCCATCTGCGGGGACCGGGCCACGGGCAA
ACACTACGGTGCCTCGAGCTGTGACGGCTGCAAGGGCTTCTTCCGGAGGAGCGTGCGGAA
GAACCACATGTACTCCTGCAGATTTAGCCGGCAGTGCGTGGTGGACAAAGACAAGAGGAA
CCAGTGCCGCTACTGCAGGCTCAAGAAATGCTTCCGGGCTGGCATGAAGAAGGAAGCCGT
CCAGAATGAGCGGGACCGGATCAGCACTCGAAGGTCAAGCTATGAGGACAGCAGCCTGCC
CTCCATCAATGCGCTCCTGCAGGCGGAGGTCCTGTCCCGACAGATCACCTCCCCCGTCTC
CGGGATCAACGGCGACATTCGGGCGAAGAAGATTGCCAGCATCGCAGATGTGTGTGAGTC
CATGAAGGAGCAGCTGCTGGTTCTCGTTGAGTGGGCCAAGTACATCCCAGCTTTCTGCGA
GCTCCCCCTGGACGACCAGGTGGCCCTGCTCAGAGCCCATGCTGGCGAGCACCTGCTGCT
CGGAGCCACCAAGAGATCCATGGTGTTCAAGGACGTGCTGCTCCTAGGCAATGACTACAT
TGTCCCTCGGCACTGCCCGGAGCTGGCGGAGATGAGCCGGGTGTCCATACGCATCCTTGA
CGAGCTGGTGCTGCCCTTCCAGGAGCTGCAGATCGATGACAATGAGTATGCCTACCTCAA
AGCCATCATCTTCTTTGACCCAGATGCCAAGGGGCTGAGCGATCCAGGGAAGATCAAGCG
GCTGCGTTCCCAGGTGCAGGTGAGCTTGGAGGACTACATCAACGACCGCCAGTATGACTC
GCGTGGCCGCTTTGGAGAGCTGCTGCTGCTGCTGCCCACCTTGCAGAGCATCACCTGGCA
GATGATCGAGCAGATCCAGTTCATCAAGCTCTTCGGCATGGCCAAGATTGACAACCTGTT
GCAGGAGATGCTGCTGGGAGGGTCCCCCAGCGATGCACCCCATGCCCACCACCCCCTGCA
CCCTCACCTGATGCAGGAACATATGGGAACCAACGTCATCGTTGCCAACACAATGCCCAC
TCACCTCAGCAACGGACAGATGTGTGAGTGGCCCCGACCCAGGGGACAGGCAGCCACCCC
TGAGACCCCACAGCCCTCACCGCCAGGTGGCTCAGGGTCTGAGCCCTATAAGCTCCTGCC
GGGAGCCGTCGCCACAATCGTCAAGCCCCTCTCTGCCATCCCCCAGCCGACCATCACCAA
GCAGGAAGTTATCTAGCAAGCCGCTGGGGCTTGGGGGCTCCACTGGCTCCCCCCAGCCCC
CTAAGAGAGCACCTGGTGATCACGTGGTCACGGCAAAGGAAGACGTGATGCCAGGACCAG
TCCCAGAGCAGGAATGGGAAGGATGAAGGGCCCGAGAACATGGCCTAAGGGCCACATCCC
ACTGCCACCCTTGACGCCCTGCTCTGGATAACAAGACTTTGACTTGGGGAGACCTCTACT
GCCTTGGACAACTTTTCTCATGTTGAAGCCACTGCCTTCACCTTCACCTTCATCCATGTC
CAACCCCCGACTTCATCC
>OriGene 5' read for NM_000457 unedited
CGCTCATTCTGGACGGCTTCCTTCTTCATGCCAGCCCGGAAGCATTTCTTGAGCCTGCAG
TAGCGGCACTGGTTCCTCTTGTCTTTGTCCACCACGCACTGCCGGCTAAATCTGCAGGAG
TACATGTGGTTCTTCCGCACGCTCCTCCGGAAGAAGCCCTTGCAGCCGTCACAGCTCGAG
GCACCGTAGTGTTTGCCCGTGGCCCGGTCCCCGCAGATGGCACACAGGGCGCTGACACCC
AGGCTGTTGGGCGCGTTGAGGTTGGTGCCTTCTGATGGGGACGTGTCATTGCCCATCGTC
AACACCTGCACATTCTCAAATTCCAGGGTGGTGTAGGCTGGGTCCAGTGCAGCACTGTAG
TCGGCCATGTCCATGTCGACGAGGGTTTTGGAGAGTCGCATTCTCCCTGCCTCCACGCCG
CGGCCACCTGCCCTACCCTGGGCGCCCACCCCGAAGGCCCCCGCCCTCCGCCCTCCCACT
GCCTCCTCCCAGTGCCCTCTCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATT
ACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGC
CTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGA
TTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGT
GAGTCANACCGCTATCCACGC
>OriGene 3' read for NM_000457 unedited
GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCC
TTAATATTTATCAGCAAACAGGATGCTCGACCCTAAGTGAATTTTAGTGCAGCCTCAGGC
CAATCTTGGTCCTGGGAGTGGGCAGGGTTCCCAGAAGAACGAGTCTGGTTTCTGAGGCTG
TAGAAGGGAGCCGGAAGCCCCTCAACTTGATCACGGTGAGAACACAGGGAGCCTTTGGAG
AAGCTATTCAGCCACTGTAGTTAAGAGCTCCTGTTCTGATCCAGGGAGACCTGGGTTCAA
GTCCTGACTCAGCCACTTCCTAGTTGTGTGAGTTTCAGAAAAAAAATCACTTCACCTCTT
AGAACGCAATTTCAGCTTCTGTAACAATCTCTAGGTTAGGAGGGAGTGGGGCGGGTGAGG
GCGGGGAGGGAGGAGGAGAATAAAAAACAAAAACAATCTCTAGGTTAATAGGGAGGAAGG
GAGGATTAAATGAGATGATGCATGTCAGATGCCTTAAGACAGTGCCTGGGAGTAAGGAAG
AGCTTGAGACAGGCCCTGGGAGCTTATTCCTCCTGGATGTCACTCTGATGTGAGGGTTTA
CCCATCTTGTCCTCTCCAGCCCCAAGCCTCATTACTCTCACCCACATTATATCATCCACC
CCACCTGACCTCCCAGCTTC
Restriction Sites Please inquire     
ACCN NM_000457
Insert Size 3300 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000457.3, NP_000448.3
RefSeq Size 3239 bp
RefSeq ORF 1425 bp
Locus ID 3172
Cytogenetics 20q13.12
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Nuclear Hormone Receptor, Transcription Factors
Protein Pathways Maturity onset diabetes of the young
Gene Summary 'The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]'
Transcript Variant: This variant (2) encodes the longest isoform (2, also known as HNF4alpha2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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