HNF 4 alpha (HNF4A) (NM_000457) Human Untagged Clone

CAT#: SC123863

HNF4A (untagged)-Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HNF4A
• Synonyms: FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000457 edited
  AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCG
  TCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGAGAGGGCA
  CTGGGAGGAGGCAGTGGGAGGGCGGAGGGCGGGGGCCTTCGGGGTGGGCGCCCAGGGTAG
  GGCAGGTGGCCGCGGCGTGGAGGCAGGGAGAATGCGACTCTCCAAAACCCTCGTCGACAT
  GGACATGGCCGACTACAGTGCTGCACTGGACCCAGCCTACACCACCCTGGAATTTGAGAA
  TGTGCAGGTGTTGACGATGGGCAATGACACGTCCCCATCAGAAGGCACCAACCTCAACGC
  GCCCAACAGCCTGGGTGTCAGCGCCCTGTGTGCCATCTGCGGGGACCGGGCCACGGGCAA
  ACACTACGGTGCCTCGAGCTGTGACGGCTGCAAGGGCTTCTTCCGGAGGAGCGTGCGGAA
  GAACCACATGTACTCCTGCAGATTTAGCCGGCAGTGCGTGGTGGACAAAGACAAGAGGAA
  CCAGTGCCGCTACTGCAGGCTCAAGAAATGCTTCCGGGCTGGCATGAAGAAGGAAGCCGT
  CCAGAATGAGCGGGACCGGATCAGCACTCGAAGGTCAAGCTATGAGGACAGCAGCCTGCC
  CTCCATCAATGCGCTCCTGCAGGCGGAGGTCCTGTCCCGACAGATCACCTCCCCCGTCTC
  CGGGATCAACGGCGACATTCGGGCGAAGAAGATTGCCAGCATCGCAGATGTGTGTGAGTC
  CATGAAGGAGCAGCTGCTGGTTCTCGTTGAGTGGGCCAAGTACATCCCAGCTTTCTGCGA
  GCTCCCCCTGGACGACCAGGTGGCCCTGCTCAGAGCCCATGCTGGCGAGCACCTGCTGCT
  CGGAGCCACCAAGAGATCCATGGTGTTCAAGGACGTGCTGCTCCTAGGCAATGACTACAT
  TGTCCCTCGGCACTGCCCGGAGCTGGCGGAGATGAGCCGGGTGTCCATACGCATCCTTGA
  CGAGCTGGTGCTGCCCTTCCAGGAGCTGCAGATCGATGACAATGAGTATGCCTACCTCAA
  AGCCATCATCTTCTTTGACCCAGATGCCAAGGGGCTGAGCGATCCAGGGAAGATCAAGCG
  GCTGCGTTCCCAGGTGCAGGTGAGCTTGGAGGACTACATCAACGACCGCCAGTATGACTC
  GCGTGGCCGCTTTGGAGAGCTGCTGCTGCTGCTGCCCACCTTGCAGAGCATCACCTGGCA
  GATGATCGAGCAGATCCAGTTCATCAAGCTCTTCGGCATGGCCAAGATTGACAACCTGTT
  GCAGGAGATGCTGCTGGGAGGGTCCCCCAGCGATGCACCCCATGCCCACCACCCCCTGCA
  CCCTCACCTGATGCAGGAACATATGGGAACCAACGTCATCGTTGCCAACACAATGCCCAC
  TCACCTCAGCAACGGACAGATGTGTGAGTGGCCCCGACCCAGGGGACAGGCAGCCACCCC
  TGAGACCCCACAGCCCTCACCGCCAGGTGGCTCAGGGTCTGAGCCCTATAAGCTCCTGCC
  GGGAGCCGTCGCCACAATCGTCAAGCCCCTCTCTGCCATCCCCCAGCCGACCATCACCAA
  GCAGGAAGTTATCTAGCAAGCCGCTGGGGCTTGGGGGCTCCACTGGCTCCCCCCAGCCCC
  CTAAGAGAGCACCTGGTGATCACGTGGTCACGGCAAAGGAAGACGTGATGCCAGGACCAG
  TCCCAGAGCAGGAATGGGAAGGATGAAGGGCCCGAGAACATGGCCTAAGGGCCACATCCC
  ACTGCCACCCTTGACGCCCTGCTCTGGATAACAAGACTTTGACTTGGGGAGACCTCTACT
  GCCTTGGACAACTTTTCTCATGTTGAAGCCACTGCCTTCACCTTCACCTTCATCCATGTC
  CAACCCCCGACTTCATCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000457 unedited
  CGCTCATTCTGGACGGCTTCCTTCTTCATGCCAGCCCGGAAGCATTTCTTGAGCCTGCAG
  TAGCGGCACTGGTTCCTCTTGTCTTTGTCCACCACGCACTGCCGGCTAAATCTGCAGGAG
  TACATGTGGTTCTTCCGCACGCTCCTCCGGAAGAAGCCCTTGCAGCCGTCACAGCTCGAG
  GCACCGTAGTGTTTGCCCGTGGCCCGGTCCCCGCAGATGGCACACAGGGCGCTGACACCC
  AGGCTGTTGGGCGCGTTGAGGTTGGTGCCTTCTGATGGGGACGTGTCATTGCCCATCGTC
  AACACCTGCACATTCTCAAATTCCAGGGTGGTGTAGGCTGGGTCCAGTGCAGCACTGTAG
  TCGGCCATGTCCATGTCGACGAGGGTTTTGGAGAGTCGCATTCTCCCTGCCTCCACGCCG
  CGGCCACCTGCCCTACCCTGGGCGCCCACCCCGAAGGCCCCCGCCCTCCGCCCTCCCACT
  GCCTCCTCCCAGTGCCCTCTCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATT
  ACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGC
  CTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGA
  TTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGT
  GAGTCANACCGCTATCCACGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000457 unedited
  GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCC
  TTAATATTTATCAGCAAACAGGATGCTCGACCCTAAGTGAATTTTAGTGCAGCCTCAGGC
  CAATCTTGGTCCTGGGAGTGGGCAGGGTTCCCAGAAGAACGAGTCTGGTTTCTGAGGCTG
  TAGAAGGGAGCCGGAAGCCCCTCAACTTGATCACGGTGAGAACACAGGGAGCCTTTGGAG
  AAGCTATTCAGCCACTGTAGTTAAGAGCTCCTGTTCTGATCCAGGGAGACCTGGGTTCAA
  GTCCTGACTCAGCCACTTCCTAGTTGTGTGAGTTTCAGAAAAAAAATCACTTCACCTCTT
  AGAACGCAATTTCAGCTTCTGTAACAATCTCTAGGTTAGGAGGGAGTGGGGCGGGTGAGG
  GCGGGGAGGGAGGAGGAGAATAAAAAACAAAAACAATCTCTAGGTTAATAGGGAGGAAGG
  GAGGATTAAATGAGATGATGCATGTCAGATGCCTTAAGACAGTGCCTGGGAGTAAGGAAG
  AGCTTGAGACAGGCCCTGGGAGCTTATTCCTCCTGGATGTCACTCTGATGTGAGGGTTTA
  CCCATCTTGTCCTCTCCAGCCCCAAGCCTCATTACTCTCACCCACATTATATCATCCACC
  CCACCTGACCTCCCAGCTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_000457
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000457.3, NP_000448.3
• RefSeq Size: 3239 bp
• RefSeq ORF: 1425 bp
• Locus ID: 3172
• Cytogenetics: 20q13.12
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: 'The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]'
  Transcript Variant: This variant (2) encodes the longest isoform (2, also known as HNF4alpha2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.