CYP27B1 (NM_000785) Human Untagged Clone

CAT#: SC123873

CYP27B1 (untagged)-Human cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP27B1
• Synonyms: CP2B; CYP1; CYP1alpha; CYP27B; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000785 edited
  GGCGGCCGCGAATTCGGCACGAGGCTGAACCAGACCATGACCCAGACCCTCAAGTACGCC
  TCCAGAGTGTTCCATCGCGTCCGCTGGGCGCCCGAGTTGGGCGCCTCCCTAGGCTACCGA
  GAGTACCACTCAGCACGCCGGAGCTTGGCAGACATCCCAGGCCCCTCTACGCCCAGCTTT
  CTGGCCGAACTTTTCTGCAAGGGGGGGCTGTCGAGGCTACACGAGCTGCAGGTGCAGGGC
  GCCGCGCACTTCGGGCCGGTGTGGCTAGCCAGCTTTGGGACAGTGCGCACCGTGTACGTG
  GCTGCCCCTGCACTCGTCGAGGAGCTGCTGCGACAGGAGGGACCCCGGCCCGAGCGCTGC
  AGCTTCTCGCCCTGGACGGAGCACCGCCGCTGCCGCCAGCGGGCTTGCGGACTGCTCACT
  GCGGAAGGCGAAGAATGGCAAAGGCTCCGCAGTCTCCTGGCCCCGCTCCTCCTCCGGCCT
  CAAGCGGCCGCCCGCTACGCCGGAACCCTGAACAACGTAGTCTGCGACCTTGTGCGGCGT
  CTGAGGCGCCAGCGGGGACGTGGCACGGGGCCGCCCGCCCTGGTTCGGGACGTGGCGGGG
  GAATTTTACAAGTTCGGACTGGAAGGCATCGCCGCGGTTCTGCTCGGCTCGCGCTTGGGC
  TGCCTGGAGGCTCAAGTGCCACCCGACACGGAGACCTTCATCCGCGCTGTGGGCTCGGTG
  TTTGTGTCCACGCTGTTGACCATGGCGATGCCCCACTGGCTGCGCCACCTTGTGCCTGGG
  CCCTGGGGCCGCCTCTGCCGAGACTGGGACCAGATGTTTGCATTTGCTCAGAGGCACGTG
  GAGCGGCGAGAGGCAGAGGCAGCCATGAGGAACGGAGGACAGCCCGAGAAGGACCTGGAG
  TCTGGGGCGCACCTGACCCACTTCCTGTTCCGGGAAGAGTTGCCTGCCCAGTCCATCCTG
  GGAAATGTGACAGAGTTGCTATTGGCGGGAGTGGACACGGTGTCCAACACGCTCTCTTGG
  GCTCTGTATGAGCTCTCCCGGCACCCCGAAGTCCAGACAGCACTCCACTCAGAGATCACA
  GCTGCCCTGAGCCCTGGCTCCAGTGCCTACCCCTCAGCCACTGTTCTGTCCCAGCTGCCC
  CTGCTGAAGGCGGTGGTCAAGGAAGTGCTAAGACTGTACCCTGTGGTACCTGGAAATTCT
  CGTGTCCCAGACAAAGACATTCATGTGGGTGACTATATTATCCCCAAAAATACGCTGGTC
  ACTCTGTGTCACTATGCCACTTCAAGGGACCCTGCCCAGTTCCCAGAGCCAAATTCTTTT
  CGTCCAGCTCGCTGGCTGGGGGAGGGTCCCACCCCCCACCCATTTGCATCTCTTCCCTTT
  GGCTTTGGCAAGCGCAGCTGTATGGGGAGACGCCTGGCAGAGCTTGAATTGCAAATGGCT
  TTGGCCCAGATCCTAACACATTTTGAGGTGCAGCCTGAGCCAGGTGCGGCCCCAGTTAGA
  CCCAAGACCCGGACTGTCCTGGTACCTGAAAGGAGCATCAACCTACAGTTTTTGGACAGA
  TAGTCCCATGGAAAGAGACTGTCATCATCACCCTTTCATTCATCATAGGGATAAGATTTT
  TTGTAGGCACAAGACCAAGGTATACATCTTCCCCTAATGCCTATCTGACCAAACTGGATA
  GAACCACCATAGTGAAGTGTGAGGCGGCCCTGACCAATGTGTGAAGTATGCACTTGGCCT
  GACTCAGGAAGCCAGGTGAGAAAACCATGGTCTCTCTGCTTGCTTGGCCCTTCTGATCAT
  GTATGCATCCCCCAAGGATGAAATCAGATTTTAACTAATAATGCTGGATGGCCTGAGGAA
  AGATTCAACTGCCTCTCTTTTTGGGCTTTCATAGTGTTCATTGATGCTGCTGGCTAAGCA
  TTTATCAAAGCATAAGCTCAGTAACTGTGCATCTGGTCTGTACCTGGTTGGTCCTTCGTC
  TTTGCATGTAAGCTCTTTGAGAGGAAGGGTGAAGCCTTATTTGTTTTTTATGTCCCCTGC
  CAGGGCCTGTCTCTGACTAGGTGTCACCATACACATTCTTAGATTGAATCTGAACCATGT
  GGCAGAAGGGATAAGCAGCTTACTTAGTAGGCTCTGTCTACCCCCTTCCTTCTTTGTCTT
  GCCCCTAGGAAGGTGAATCTGCCCTAGCCTGGTTTACGGTTTCTTATAACTCTCCTTTGC
  TCTCTGGCCACTATTAAGTGGGTTTGCCCCATCACTTAGTTCTCAGGCAGAGACATCTTT
  GGGCCTGTCCCTGCCCAGGCCTCTGGCTTTTTATATTGAAAATTTTTAAATATTCACAAA
  TTTTAGAATAAATCAAATATTCCATTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGC
  GGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCC
  AGTGCCTC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000785 unedited
  NGGGTTCCCAATTTGTATACGACNACTATAGGCGGCCGCGNAATTCGCACGAGGCTGAAC
  CAGACCTGACCCAGACCCTCAGTACGCCTCCAGAGTGTTCCATCGCGTCCGCTGGGCGCC
  CGAGTTGGGCGCCTCCCTAGGCTACCGAGAGTACCACTCAGCACGCCGGAGCTTGGCAGA
  CATCCCAGGCCCCTCTACGCCCAGCTTTCTGGCCGAACTTTTCTGCAAGGGGGGGCTGTC
  GAGGCTACACGAGCTGCAGGTGCAGGGCGCCGCGCACTTCGGGCCGGTGTGGCTAGCCAG
  CTTTGGGACAGTGCGCACCGTGTACGTGGCTGCCCCTGCACTCGTCGAGGAGCTGCTGCG
  ACAGGAGGGACCCCGGCCCGAGCGCTGCAGCTTCTCGCCCTGGACGGAGCACCGCCGCTG
  CCGCCAGCGGGCTTGCGGACTGCTCACTGCGGAAGGCGAAGAATGGCAAAGGCTCCGCAG
  TCTCCTGGCCCCGCTCCTCCTCCGGCCTCAAGCGGCCGCCCGCTACGCCGGAACCCTGAA
  CAACGTAGTCTGCGACCTTGTGCGGCGTCTGAGGCGCCAGCGGGGACGTGGCACGGGGCC
  GCCCGCCCTGGTTCGGGACGTGGCGGGGGAATTTTACAAGTTCGGACTGGAAGGCATCGC
  CGCGGTTCTGCTCGGCTCGCGCTTGGGCTGCCTGGAGGCTCAAGTGCCACCCGACACGGA
  GACCTTCATCCGCGCTGTGGGCTCGGTGTTTGTGTCCACGCTGTTGACCATGGCGATGCC
  CCACTGGCTGCGCCACCTTGTGCCTGGGCCCTGGGGCCGCCTCTGCCGAGACTGGGACCA
  GATGTTTGCATTTGCTCAGAGCACNNTGGAGCGCNAGAGGCANAAGCAGCCATNGAGAAC
  GGNAGACAGCCCGAGAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000785 unedited
  GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATGGAATATTTGAT
  TTATTCTAAAATTTGTGAATATTTAAAAATTTTCAATATAAAAAGCCAGAGGCCTGGGCA
  GGGACAGGCCCAAAGATGTCTCTGCCTGAGAACTAAGTGATGGGGCAAACCCACTTAATA
  GTGGCCAGAGAGCAAAGGAGAGTTATAAGAAACCGTAAACCAGGCTAGGGCAGATTCACC
  TTCCTAGGGGCAAGACAAAGAAGGAAGGGGGTAGACAGAGCCTACTAAGTAAGCTGCTTA
  TCCCTTCTGCCACATGGTTCAGATTCAATCTAAGAATGTGTATGGTGACACCTAGTCAGA
  GACAGGCCCTGGCAGGGGACATAAAAAACAAATAAGGCTTCACCCTTCCTCTCAAAGAGC
  TTACATGCAAAGACGAAGGACCAACCAGGTACAGACCAGATGCACAGTTACTGAGCTTAT
  GCTTTGATAAATGCTTAGCCAGCAGCATCAATGAACACTATGAAAGCCCAAAAAGAGAGG
  CAGTTGAATCTTTCCTCAGGCCATCCAGCATTATTAGTTAAAATCTGATTTCATCCTTGG
  NGGATGCATACATGATCAGAAGGGCCAAGCAAGCAGAGAGACCATGGTTTTCTCACCTGG
  CTTCCTGAGTCAGGCCAAGTGCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000785
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000785.3, NP_000776.1
• RefSeq Size: 2503 bp
• RefSeq ORF: 1527 bp
• Locus ID: 1594
• Cytogenetics: 12q14.1
• Protein Families: Druggable Genome, P450
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]'