VR1 (TRPV1) (NM_018727) Human Untagged Clone

CAT#: SC123895

TRPV1 (untagged)-Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 2


  "NM_018727" in other vectors (6)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TRPV1
Synonyms VR1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_018727 edited
ATGAAGAAATGGAGCAGCACAGACTTGGGGGCAGCTGCGGACCCACTCCAAAAGGACACC
TGCCCAGACCCCCTGGATGGAGACCCTAACTCCAGGCCACCTCCAGCCAAGCCCCAGCTC
TCCACGGCCAAGAGCCGCACCCGGCTCTTTGGGAAGGGTGACTCGGAGGAGGCTTTCCCA
GTGGATTGCCCTCACGAGGAAGGTGAGCTGGACTCCTGCCCGACCATCACAGTCAGCCCT
GTTATCACCATCCAGAGGCCAGGAGACGGCCCCACCGGTGCCAGGCTGCTGTCCCAGGAC
TCTGTCGCCGCCAGCACCGAGAAGACCCTCAGGCTCTATGATCGCAGGAGTATCTTTGAA
GCCGTTGCTCAGAATAACTGCCAGGATCTGGAGAGCCTGCTGCTCTTCCTGCAGAAGAGC
AAGAAGCACCTCACAGACAACGAGTTCAAAGACCCTGAGACAGGGAAGACCTGTCTGCTG
AAAGCCATGCTCAACCTGCACGACGGACAGAACACCACCATCCCCCTGCTCCTGGAGATC
GCGCGGCAAACGGACAGCCTGAAGGAGCTTGTCAACGCCAGCTACACGGACAGCTACTAC
AAGGGCCAGACAGCACTGCACATCGCCATCGAGAGACGCAACATGGCCCTGGTGACCCTC
CTGGTGGAGAACGGAGCAGACGTCCAGGCTGCGGCCCATGGGGACTTCTTTAAGAAAACC
AAAGGGCGGCCTGGATTCTACTTCGGTGAACTGCCCCTGTCCCTGGCCGCGTGCACCAAC
CAGCTGGGCATCGTGAAGTTCCTGCTGCAGAACTCCTGGCAGACGGCCGACATCAGCGCC
AGGGACTCGGTGGGCAACACGGTGCTGCACGCCCTGGTGGAGGTGGCCGACAACACGGCC
GACAACACGAAGTTTGTGACGAGCATGTACAATGAGATTCTGATGCTGGGGGCCAAACTG
CACCCGACGCTGAAGCTGGAGGAGCTCACCAACAAGAAGGGAATGACGCCGCTGGCTCTG
GCAGCTGGGACCGGGAAGATCGGGGTCTTGGCCTATATTCTCCAGCGGGAGATCCAGGAG
CCCGAGTGCAGGCACCTGTCCAGGAAGTTCACCGAGTGGGCCTACGGGCCCGTGCACTCC
TCGCTGTACGACCTGTCCTGCATCGACACCTGCGAGAAGAACTCGGTGCTGGAGGTGATC
GCCTACAGCAGCAGCGAGACCCCTAATCGCCACGACATGCTCTTGGTGGAGCCGCTGAAC
CGACTCCTGCAGGACAAGTGGGACAGATTCGTCAAGCGCATCTTCTACTTCAACTTCCTG
GTCTACTGCCTGTACATGATCATCTTCACCATGGCTGCCTACTACAGGCCCGTGGATGGC
TTGCCTCCCTTTAAGATGGAAAAAATTGGAGACTATTTCCGAGTTACTGGAGAGATCCTG
TCTGTGTTAGGAGGAGTCTACTTCTTTTTCCGAGGGATTCAGTATTTCCTGCAGAGGCGG
CCGTCGATGAAGACCCTGTTTGTGGACAGCTACAGTGAGATGCTTTTCTTTCTGCAGTCA
CTGTTCATGCTGGCCACCGTGGTGCTGTACTTCAGCCACCTCAAGGAGTATGTGGCTTCC
ATGGTATTCTCCCTGGCCTTGGGCTGGACCAACATGCTCTACTACACCCGCGGTTTCCAG
CAGATGGGCATCTATGCCGTCATGATAGAGAAGATGATCCTGAGAGACCTGTGCCGTTTC
ATGTTTGTCTACGTCGTCTTCTTGTTCGGGTTTTCCACAGCGGTGGTGACGCTGATTGAA
GACGGGAAGAATGACTCCCTGCCGTCTGAGTCCACGTCGCACAGGTGGCGGGGGCCTGCC
TGCAGGCCCCCCGATAGCTCCTACAACAGCCTGTACTCCACCTGCCTGGAGCTGTTCAAG
TTCACCATCGGCATGGGCGACCTGGAGTTCACTGAGAACTATGACTTCAAGGCTGTCTTC
ATCATCCTGCTGCTGGCCTATGTAATTCTCACCTACATCCTCCTGCTCAACATGCTCATC
GCCCTCATGGGTGAGACTGTCAACAAGATCGCACAGGAGAGCAAGAACATCTGGAAGCTG
CAGAGAGCCATCACCATCCTGGACACGGAGAAGAGCTTCCTTAAGTGCATGAGGAAGGCC
TTCCGCTCAGGCAAGCTGCTGCAGGTGGGGTACACACCTGATGGCAAGGACGACTACCGG
TGGTGCTTCAGGGTGGACGAGGTGAACTGGACCACCTGGAACACCAACGTGGGCATCATC
AACGAAGACCCGGGCAACTGTGAGGGCGTCAAGCGCACCCTGAGCTTCTCCCTGCGGTCA
AGCAGAGTTTCAGGCAGACACTGGAAGAACTTTGCCCTGGTCCCCCTTTTAAGAGAGGCA
AGTGCTCGAGATAGGCAGTCTGCTCAGCCCGAGGAAGTTTATCTGCGACAGTTTTCAGGG
TCTCTGAAGCCAGAGGACGCTGAGGTCTTCAAGAGTCCTGCCGCTTCCGGGGAGAAGTGA
>OriGene 5' read for NM_018727 unedited
AGGGGNNATGGGNNNTNNNNTTGAGTCAGATTTTGTAATACGACTACTATAGGGCGGCCG
CGAATTCGGCACGAGAGACACCAGAAAGTCTGGTGGCTTTGGTCCCAAGTGGGAAAAGAG
AACTGCCCCATGCCCAGCTTGTGATTATCGTTTTTGGAGACCTGAAGCCCACACTCGGGT
CGTATGGACTTCTGGAAAAGTTCTTGTCTCCTGGACTGAACCATGTGACCGGAGGCCCCT
TTCCTAGTCTCATCCTCCCCTGGCTGCAGATGCTTAGCTGGGCCAGGGATTGACCCAAGC
GCGATGCAGCAGGCAGGCTCAGAAGACGATGCGGGGCTGTGTGCCGGCCTTCTTGCTGCA
TGTACTCAGCCTCAGGAGAGCTTGCTGCACCCAGGCCGCCCAGGTCTTCACAGCACAACT
GCCTGGAAGGCAGGTTGCGAGAAGGAGAGGCGGATGGCATGAGCAGCAAGGGGGACCGAT
GCTGTGCAGCTCACACCACTCCAGAACCTGACAAGGCACCAGCAGGACCCCTTGCCAGGA
GCATGTCTGTGCAGCAGTGTTTTTGCCCCTGCACATTCCAGAAGCCCTCATGGGAAGGGA
TGCAGCCAGGCAGACTCCTGCCAGATGGGGCAGGTTGCACACTGNGCCACAGAGGATCCA
GCAAGGATGAAGAAATGGAGCAGCACAGACTTGGGGGCAGCTGCGGACCCACTCCAAAAG
GACACCTGCCCAGACCCCCTGGATGGAGACCCTAACTTCAGGCCACTTCCAGCCAGCCCC
AGCTCTCCACGCCAA
>OriGene 3' read for NM_018727 unedited
AGGGGTGGNNTNNNTTGCCTGGATGGCACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGT
CACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGA
GTCGAGTTTTTTTTTTTTTTTTTTATGTATACATATCTGTTTATTTGAGGATGCCAGTAT
GGATGGAGTGGAAGCATGTGCCATTGCGGAGAAACAGGACTGGGGTTCCTAGAAATGGAA
GATCTTTCAAACATTCTTACCAGGTACCTTTGGGATGTGGTTCTGTAGTTTTAACCTGCA
CAGCAATTGAGCTGATCTTCTGGAGACTGTGATTGATCGTAAGGAAGGATGAAGAAAGCA
CTGCTGCAACAGCTTGATTCTGCCAGAGCTGGGAGTGGGACAGCCTCCCCCATCTTCTCC
AGGGAGGAAACACCAACACCCCCAAACCACCCGGGCAGGTAAGAGGAGCAAGCACGGCAC
AGAGAGGAAGGGCCTCTGCATTTTCCATCAAAGGAAGAGTTTGTTCCCAAAGGTGTTTTC
CTGGGCTTCATTTACTTTTGCTCCTAATAATATAAAATCAAAGAAGGCCGAGCGCAGCGG
CTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCAGATCACCTGAGGTCAAG
AGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTATTAAAAATACAAAAATT
AGCCGGGCGTGGTAGTGCTCACCTGTAATCCAAGCTACTTGGGAGACTGAAGCAGAAGAA
TCGCTTG
Restriction Sites Please inquire     
ACCN NM_018727
Insert Size 5000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018727.4, NP_061197.4
RefSeq Size 4046 bp
RefSeq ORF 2520 bp
Locus ID 7442
Cytogenetics 17p13.2
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
Gene Summary 'Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) contains a different segment in the 5' UTR, compared to variant 3. Variants 1-4 all encode the same protein.

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