MMP12 (NM_002426) Human Untagged Clone

CAT#: SC123937

MMP12 (untagged)-Human matrix metallopeptidase 12 (macrophage elastase) (MMP12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MMP12
• Synonyms: HME; ME; MME; MMP-12
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002426 edited
  AATTGATCCGTTTAGAAGTTTACAATGAAGTTTCTTCTAATACTGCTCCTGCAGGCCACT
  GCTTCTGGAGCTCTTCCCCTGAACAGCTCTACAAGCCTGGAAAAAAATAATGTGCTATTT
  GGTGAAAGATACTTAGAAAAATTTTATGGCCTTGAGATAAACAAACTTCCAGTGACAAAA
  ATGAAATATAGTGGAAACTTAATGAAGGAAAAAATCCAAGAAATGCAGCACTTCTTGGGT
  CTGAAAGTGACCGGGCAACTGGACACATCTACCCTGGAGATGATGCACGCACCTCGATGT
  GGAGTCCCCGATGTCCATCATTTCAGGGAAATGCCAGGGGGGCCCGTATGGAGGAAACAT
  TATATCACCTACAGAATCAATAATTACACACCTGACATGAACCGTGAGGATGTTGACTAC
  GCAATCCGGAAAGCTTTCCAAGTATGGAGTAATGTTACCCCCTTGAAATTCAGCAAGATT
  AACACAGGCATGGCTGACATTTTGGTGGTTTTTGCCCGTGGAGCTCATGGAGACTTCCAT
  GCTTTTGATGGCAAAGGTGGAATCCTAGCCCATGCTTTTGGACCTGGATCTGGCATTGGA
  GGGGATGCACATTTCGATGAGGACGAATTCTGGACTACACATTCAGGAGGCACAAACTTG
  TTCCTCACTGCTGTTCACGAGATTGGCCATTCCTTAGGTCTTGGCCATTCTAGTGATCCA
  AAGGCCGTAATGTTCCCCACCTACAAATATGTTGACATCAACACATTTCGCCTCTCTGCT
  GATGACATACGTGGCATTCAGTCCCTGTATGGAGACCCAAAAGAGAACCAACGCTTGCCA
  AATCCTGACAATTCAGAACCAGCTCTCTGTGACCCCAATTTGAGTTTTGATGCTGTCACT
  ACCGTGGGAAATAAGATCTTTTTCTTCAAAGACAGGTTCTTCTGGCTGAAGGTTTCTGAG
  AGACCAAAGACCAGTGTTAATTTAATTTCTTCCTTATGGCCAACCTTGCCATCTGGCATT
  GAAGCTGCTTATGAAATTGAAGCCAGAAATCAAGTTTTTCTTTTTAAAGATGACAAATAC
  TGGTTAATTAGCAATTTAAGACCAGAGCCAAATTATCCCAAGAGCATACATTCTTTTGGT
  TTTCCTAACTTTGTGAAAAAAATTGATGCAGCTGTTTTTAACCCACGTTTTTATAGGACC
  TACTTCTTTGTAGATAACCAGTATTGGAGGTATGATGAAAGGAGACAGATGATGGACCCT
  GGTTATCCCAAACTGATTACCAAGAACTTCCAAGGAATCGGGCCTAAAATTGATGCAGTC
  TTCTACTCTAAAAACAAATACTACTATTTCTTCCAAGGATCTAACCAATTTGAATATGAC
  TTCCTACTCCAACGTATCACCAAAACACTGAAAAGCAATAGCTGGTTTGGTTGTTAGAAA
  TGGTGTAATTAATGGTTTTTGTTAGTTCACTTCAGCTTAATAAGTATTTATTGCATATTT
  GCTATGTCCTCAGTGTACCACTACTTAGAGATATGTATCATAAAAATAAAATCTGTAAAC
  CATAGGTAATGATTATATAAAATACATAATATTTTTCAATTTTGAAAACTCTAATTGTCC
  ATTCTTGCTTGACTCTACTATTAAGTTTGAAAATAGTTACCTTCAAAGGCCAAGAGAATT
  CTATTTGAAGCATGCTCTGTAAGTTGCTTCCTAACATCCTTGGACTGAGAAATTATACTT
  ACTTCTGGCATAACTAAAATTAAGTATATATATTTTGGCTCAAATAAATTTGAAAAAAAA
  AAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002426 unedited
  TAAAGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGAATTGAT
  CCGTTTAGAAGTTTACAATGAAGTTTCTTCTAATACTGCTCCTGCAGGCCACTGCTTCTG
  GAGCTCTTCCCCTGAACAGCTCTACAAGCCTGGAAAAAAATAATGTGCTATTTGGTGAAA
  GATACTTAGAAAAATTTTATGGCCTTGAGATAAACAAACTTCCAGTGACAAAAATGAAAT
  ATAGTGGAAACTTAATGAAGGAAAAAATCCAAGAAATGCAGCACTTCTTGGGTCTGAAAG
  TGACCGGGCAACTGGACACATCTACCCTGGAGATGATGCACGCACCTCGATGTGGAGTCC
  CCGATGTCCATCATTTCAGGGAAATGCCAGGGGGGCCCGTATGGAGGAAACATTATATCA
  CCTACAGAATCAATAATTACACACCTGACATGAACCGTGAGGATGTTGACTACGCAATCC
  GGAAAGCTTTCCAAGTATGGAGTAATGTTACCCCCTTGAAATTCAGCAAGATTAACACAG
  GCATGGCTGACATTTTGGTGGTTTTTGCCCGTGGAGCTCATGGAGACTTCCATGCTTTTG
  ATGGCAAAGGTGGAATCCTAGCCCATGCTTTTGGACCTGGATCTGGCATTGGAGGGGATG
  CACATTTCGATGAGGACGAATTCTGGACTACACATTCAGGAGGCACAAACTTGGTCCTCA
  CTGCTGTTCACGAGATTGGCCATTCCTTANGTCTTGGCCATTCTAGTGATCCAAAGGCCG
  TAATGTTTCCCCCCTACCAATATGTTGACATCACACCTTTCGGCTCTCTGCTGATGACAT
  ACCTGGCATTCAGTCCCTGTATGGGGACCCCANAAAAACCCACCGCTTGCCCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002426
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_002426.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002426.2, NP_002417.2
• RefSeq Size: 1825 bp
• RefSeq ORF: 1413 bp
• Locus ID: 4321
• Cytogenetics: 11q22.2
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]'