P2X3 (P2RX3) (NM_002559) Human Untagged Clone

CAT#: SC123980

P2RX3 (untagged)-Human purinergic receptor P2X, ligand-gated ion channel, 3 (P2RX3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P2RX3
• Synonyms: P2X3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002559 edited
  CCTGTCCTGTAGGACCTCCCTCTCCTGAGGCCACCACTGGGCCCCCTTCTGAGTGTCCCC
  TGAGCACTCTCTCAGCATGAACTGCATATCCGACTTCTTCACCTATGAGACCACCAAGTC
  GGTGGTTGTGAAGAGCTGGACCATCGGGATCATCAACCGAGTAGTTCAGCTTCTGATCAT
  CTCCTACTTTGTAGGGTGGGTTTTCTTGCACGAGAAGGCTTACCAGGTACGGGACACAGC
  CATTGAGTCCTCGGTGGTAACCAAGGTGAAGGGCTCCGGACTCTACGCCAACAGAGTCAT
  GGATGTGTCTGATTACGTGACGCCACCTCAGGGCACCTCGGTCTTTGTCATCATCACCAA
  GATGATTGTTACTGAAAATCAGATGCAAGGATTCTGCCCAGAGAGTGAGGAGAAATACCG
  CTGTGTATCAGACAGCCAGTGCGGGCCTGAGCGCTTGCCAGGTGGGGGGATCCTCACTGG
  CCGCTGCGTGAACTACAGCTCTGTGCTCCGGACCTGTGAGATCCAGGGCTGGTGCCCCAC
  GGAGGTGGACACAGTGGAAACGCCCATCATGATGGAAGCTGAGAACTTCACTATTTTCAT
  CAAGAACAGCATCCGTTTCCCCCTCTTCAACTTTGAGAAGGGAAACCTCCTTCCCAACCT
  GACAGCCAGGGACATGAAGACCTGCCGCTTCCACCCGGACAAGGACCCTTTCTGCCCCAT
  CTTGCGGGTAGGGGACGTGGTCAAGTTTGCGGGGCAGGATTTTGCCAAACTGGCGCGCAC
  GGGGGGAGTTCTGGGCATTAAGATCGGCTGGGTGTGCGACTTGGACAAGGCCTGGGACCA
  GTGCATCCCCAAATACTCCTTCACCCGGCTCGACAGCGTTTCTGAGAAAAGCAGCGTGTC
  CCCAGGCTACAACTTCAGGTTTGCCAAGTACTACAAAATGGAAAATGGCAGTGAGTACCG
  CACCCTCCTGAAGGCTTTTGGCATCCGCTTCGACGTGCTGGTATACGGGAATGCTGGCAA
  GTTCAACATCATCCCCACCATCATCAGCTCTGTGGCGGCCTTTACTTCTGTGGGAGTGGG
  AACTGTTCTCTGTGACATCATCCTGCTCAACTTCCTCAAGGGGGCCGACCAGTACAAAGC
  CAAGAAGTTTGAGGAGGTGAATGAGACTACACTGAAAATCGCGGCTTTGACCAACCCAGT
  GTACCCCAGCGACCAGACCACAGTGGAGAAGCAGTCCACCGATTCGGGGGCCTTCTCCAT
  AGGCCACTAGGGCCTCTTTCCAGGGCCCCACACTCACAAAGGCTCCAGGCCTCCCCACAG
  AGGACCCTGCCTGAGCAAGGGGGCATGGGACCTGTCCTGTAGGACCTCCCTCTCCTGAGG
  CCACCACTGGGCCCCCTTCTGAGTGTCCCCTGAGCACTCTCTCAGCATGAACTGCATATC
  CGACTTCTTCACCTATGAGACCACCAAGTCGGTGGTTGTGAAGAGCTGGACCATCGGGAT
  CATCAACCGAGTAGTTCAGCTTCTGATCATCTCCTACTTTGTAGGGTGGGTTTTCTTGCA
  CGAGAAGGCTTACCAGGTACGGGACACAGCCATTGAGTCCTCGGTGGTAACCAAGGTGAA
  GGGCTCCGGACTCTACGCCAACAGAGTCATGGATGTGTCTGATTACGTGACGCCACCTCA
  GGGCACCTCGGTCTTTGTCATCATCACCAAGATGATTGTTACTGAAAATCAGATGCAAGG
  ATTCTGCCCAGAGAGTGAGGAGAAATACCGCTGTGTATCAGACAGCCAGTGCGGGCCTGA
  GCGCTTGCCAGGTGGGGGGATCCTCACTGGCCGCTGCGTGAACTACAGCTCTGTGCTCCG
  GACCTGTGAGATCCAGGGCTGGTGCCCCACGGAGGTGGACACAGTGGAAACGCCCATCAT
  GATGGAAGCTGAGAACTTCACTATTTTCATCAAGAACAGCATCCGTTTCCCCCTCTTCAA
  CTTTGAGAAGGGAAACCTCCTTCCCAACCTGACAGCCAGGGACATGAAGACCTGCCGCTT
  CCACCCGGACAAGGACCCTTTCTGCCCCATCTTGCGGGTAGGGGACGTGGTCAAGTTTGC
  GGGGCAGGATTTTGCCAAACTGGCGCGCACGGGGGGAGTTCTGGGCATTAAGATCGGCTG
  GGTGTGCGACTTGGACAAGGCCTGGGACCAGTGCATCCCCAAATACTCCTTCACCCGGCT
  CGACAGCGTTTCTGAGAAAAGCAGCGTGTCCCCAGGCTACAACTTCAGGTTTGCCAAGTA
  CTACAAAATGGAAAATGGCAGTGAGTACCGCACCCTCCTGAAGGCTTTTGGCATCCGCTT
  CGACGTGCTGGTATACGGGAATGCTGGCAAGTTCAACATCATCCCCACCATCATCAGCTC
  TGTGGCGGCCTTTACTTCTGTGGGAGTGGGAACTGTTCTCTGTGACATCATCCTGCTCAA
  CTTCCTCAAGGGGGCCGACCAGTACAAAGCCAAGAAGTTTGAGGAGGTGAATGAGACTAC
  ACTGAAAATCGCGGCTTTGACCAACCCAGTGTACCCCAGCGACCAGACCACAGTGGAGAA
  GCAGTCCACCGATTCGGGGGCCTTCTCCATAGGCCACTAGGGCCTCTTTCCAGGGCCCCA
  CACTCACAAAGGCTCCAGGCCTCCCCACAGAGGACCCTGCCTGAGCAAGGGGGCATGGGA
  CCTGTCCTGTAGGACCTCCCTCTCCTGAGGCCACCACTGGGCCCCCTTCTGAGTGTCCCC
  TGAGCACTCTCTCAGCATGAACTGCATATCCGACTTCTTCACCTATGAGACCACCAAGTC
  GGTGGTTGTGAAGAGCTGGACCATCGGGATCATCAACCGAGTAGTTCAGCTTCTGATCAT
  CTCCTACTTTGTAGGGTGGGTTTTCTTGCACGAGAAGGCTTACCAGGTACGGGACACAGC
  CATTGAGTCCTCGGTGGTAACCAAGGTGAAGGGCTCCGGACTCTACGCCAACAGAGTCAT
  GGATGTGTCTGATTACGTGACGCCACCTCAGGGCACCTCGGTCTTTGTCATCATCACCAA
  GATGATTGTTACTGAAAATCAGATGCAAGGATTCTGCCCAGAGAGTGAGGAGAAATACCG
  CTGTGTATCAGACAGCCAGTGCGGGCCTGAGCGCTTGCCAGGTGGGGGGATCCTCACTGG
  CCGCTGCGTGAACTACAGCTCTGTGCTCCGGACCTGTGAGATCCAGGGCTGGTGCCCCAC
  GGAGGTGGACACAGTGGAAACGCCCATCATGATGGAAGCTGAGAACTTCACTATTTTCAT
  CAAGAACAGCATCCGTTTCCCCCTCTTCAACTTTGAGAAGGGAAACCTCCTTCCCAACCT
  GACAGCCAGGGACATGAAGACCTGCCGCTTCCACCCGGACAAGGACCCTTTCTGCCCCAT
  CTTGCGGGTAGGGGACGTGGTCAAGTTTGCGGGGCAGGATTTTGCCAAACTGGCGCGCAC
  GGGGGGAGTTCTGGGCATTAAGATCGGCTGGGTGTGCGACTTGGACAAGGCCTGGGACCA
  GTGCATCCCCAAATACTCCTTCACCCGGCTCGACAGCGTTTCTGAGAAAAGCAGCGTGTC
  CCCAGGCTACAACTTCAGGTTTGCCAAGTACTACAAAATGGAAAATGGCAGTGAGTACCG
  CACCCTCCTGAAGGCTTTTGGCATCCGCTTCGACGTGCTGGTATACGGGAATGCTGGCAA
  GTTCAACATCATCCCCACCATCATCAGCTCTGTGGCGGCCTTTACTTCTGTGGGAGTGGG
  AACTGTTCTCTGTGACATCATCCTGCTCAACTTCCTCAAGGGGGCCGACCAGTACAAAGC
  CAAGAAGTTTGAGGAGGTGAATGAGACTACACTGAAAATCGCGGCTTTGACCAACCCAGT
  GTACCCCAGCGACCAGACCACAGTGGAGAAGCAGTCCACCGATTCGGGGGCCTTCTCCAT
  AGGCCACTAGGGCCTCTTTCCAGGGCCCCACACTCACAAAGGCTCCAGGCCTCCCCACAG
  AGGACCCTGCCTGAGCAAGGGGGCATGGGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002559 unedited
  GAATACGACAGGCTATAGGGCGGCCGCGAATTCGGCACGAGCANGAGCCNANAATCTCAC
  TAGGATTGCATGGCTTAAAGGGACAGGCTCCCCATTCCTCCAACCCCTCTAAGCTGCCCC
  CTCCAGGTCGTGATCTCGTCTCCCTGTCCTGTAGGACCTCCCTCTCCTGAGGCCACCACT
  GGGCCCCCTTCTGAGTGTCCCCTGAGCACTCTCTCAGCATGAACTGCATATCCGACTTCT
  TCACCTATGAGACCACCAAGTCGGTGGTTGTGAAGAGCTGGACCATCGGGATCATCAACC
  GAGTAGTTCAGCTTCTGATCATCTCCTACTTTGTAGGGTGGGTTTTCTTGCACGAGAAGG
  CTTACCAGGTACGGGACACAGCCATTGAGTCCTCGGTGGTAACCAAGGTGAAGGGCTCCG
  GACTCTACGCCAACAGAGTCATGGATGTGTCTGATTACGTGACGCCACCTCAGGGCACCT
  CGGTCTTTGTCATCATCACCAAGATGATTGTTACTGAAAATCAGATGCAAGGATTCTGCC
  CAGAGAGTGAGGAGAAATACCGCTGTGTATCAGACAGCCAGTGCGGGCCTGAGCGCTTGC
  CAGGTGGNGGGATCCTCACTGGCCGCTGCGTGAACTACAGCTCTGTGCTCCGGACCTGTG
  AGATCCCAGGCTGGTGCCCCACCGAAGTGGACACAGTTNAAACGCCCATCATGATTGGAG
  CTGGAAACTTTCTATTTTTATTAAAAACAGTATCGTTTCCCCTTTTAAATTGAGAAGGAA
  ACCTCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002559 unedited
  TGTACCTGGAGGCACTGGGTTGGTCAAAGCCGCGATTTTCAGTGTNGNNNNNTTCACCTC
  CTCAAACTTCTTGGCTTTGTACTGGTCGGCCCCCTTGAGGAAGTTGAGCAGGATGATGTC
  ACAGAGAACAGTTCCCACTCCCACAGAAGTAAAGGCCGCCACAGAGCTGATGATGGTGGG
  GATGATGTTGAACTTGCCAGCATTCCCGTATACCAGCACGTCGAAGCGGATGCCAAAAGC
  CTTCAGGAGGGTGCGGTACTCACTGCCATTTTCCATTTTGTAGTACTTGGCAAACCTGAA
  GTTGTAGCCTGGGGACACGCTGCTTTTCTCAGAAACGCTGTCGAGCCGGGTGAAGGAGTA
  TTTGGGGATGCACTGGTCCCAGGCCTTGTCCAAGTCGCACACCCAGCCGATCTTAATGCC
  CAGAACTCCCCCCGTGCGCGCCAGTTTGGCAAAATCCTGCCCCGCAAACTTGACCACGTC
  CCCTACCCGCAAGATGGGGCAGAAAGGGTCCTTGTCCGGGTGGAAGCGGCAGGTCTTCAT
  GTCCCTGGCTGTCAGGTTGGGAAGGAGGTTTCCCTTCTCAAAGTTGAAGAGGGGGAAACG
  GATGCTGTTCTTGATGAAAATAGTGAAGTTCTCAGCTTCCATCATGATGGGCGTTTCCAC
  TGTGTCCACCTCCGTGGGGCACCAGCCCTGGATCTCACAGGTCCAGAGCACAGAGCTGTA
  GTTCACGCAGCGGCCAGTGAGGATCCCNCCACCTGCAGCGCTCAGCCCGCACTGCTGTCT
  GATCACAGCGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_002559
• Insert Size: 3800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002559.2, NP_002550.2
• RefSeq Size: 1349 bp
• RefSeq ORF: 1194 bp
• Locus ID: 5024
• Cytogenetics: 11q12.1
• Protein Families: Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes a member of the P2X purinergic receptor (purinoceptor) gene family which includes seven members (P2RX1 - P2RX7). P2X purinoceptors are a family of cation-permeable, ligand-gated ion channels that open in response to the binding of extracellular adenosine 5'-triphosphate (ATP). The encoded protein is a subunit of the trimeric P2X3 receptor ion channel which is expressed by sensory or autonomic neurons. A deficiency of the orthologous protein in mice is associated with reduced pain-related behavior and urinary bladder hyporeflexia. [provided by RefSeq, Aug 2017]'