Pepsinogen II (PGC) (NM_002630) Human Untagged Clone

CAT#: SC123989

PGC (untagged)-Human progastricsin (pepsinogen C) (PGC), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PGC
• Synonyms: PEPC; PGII
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC123989 sequence for NM_002630 edited (data generated by NextGen Sequencing)
  ATGAAGTGGATGGTGGTGGTCTTGGTCTGCCTCCAGCTCTTGGAGGCAGCAGTGGTCAAA
  GTGCCCCTGAAGAAATTTAAGTCTATCCGTGAGACCATGAAGGAGAAGGGCTTGCTGGGG
  GAGTTCCTGAGGACCCACAAGTATGATCCTGCTTGGAAGTACCGCTTTGGTGACCTCAGC
  GTGACCTACGAGCCCATGGCCTACATGGATGCTGCCTACTTTGGTGAGATCAGCATCGGG
  ACTCCACCCCAGAACTTCCTGGTCCTTTTTGACACCGGCTCCTCCAACTTGTGGGTGCCC
  TCTGTCTACTGCCAGAGCCAGGCCTGCACCAGTCACTCCCGCTTCAACCCCAGCGAGTCG
  TCCACCTACTCCACCAATGGGCAGACCTTCTCCCTGCAGTATGGCAGTGGCAGCCTCACC
  GGCTTCTTTGGCTATGACACCCTGACTGTCCAGAGCATCCAGGTCCCCAACCAGGAGTTC
  GGCTTGAGTGAGAATGAGCCTGGTACCAACTTCGTCTATGCGCAGTTTGATGGCATCATG
  GGCCTGGCCTACCCTGCTCTGTCCGTGGATGAGGCCACCACAGCTATGCAGGGCATGGTG
  CAGGAGGGCGCCCTCACCAGCCCCGTCTTCAGCGTCTACCTCAGCAACCAGCAGGGCTCC
  AGCGGGGGAGCGGTTGTCTTTGGGGGTGTGGATAGCAGCCTGTACACGGGGCAGATCTAC
  TGGGCGCCTGTCACCCAGGAACTCTACTGGCAGATTGGCATTGAAGAGTTCCTCATCGGC
  GGCCAGGCCTCCGGCTGGTGTTCTGAGGGTTGCCAGGCCATCGTGGACACAGGCACCTCT
  CTGCTCACTGTGCCCCAGCAGTACATGAGTGCTCTTCTGCAGGCCACAGGGGCCCAGGAG
  GATGAGTATGGACAGTTTCTCGTGAACTGTAACAGCATTCAGAATCTGCCCAGCTTGACC
  TTCATCATCAATGGTGTGGAGTTCCCTCTGCCACCTTCCTCCTATATCCTCAGTAACAAC
  GGCTACTGCACCGTGGGAGTCGAGCCCACCTACCTGTCCTCCCAGAACGGCCAGCCCCTG
  TGGATCCTCGGGGATGTCTTCCTCAGGTCCTACTATTCCGTCTACGACTTGGGCAACAAC
  AGAGTAGGCTTTGCCACTGCCGCCTAG
  
  Clone variation with respect to NM_002630.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002630 unedited
  CAGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGNAACTCAGAGC
  TGCTCTTCCTCTGTGGCCAGTTGGGGACCAGCATCATGAAGTGGATGGTGGTGGTCTTGG
  TCTGCCTCCAGCTCTTGGAGGCAGCAGTGGTCAAAGTGCCCCTGAAGAAATTTAAGTCTA
  TCCGTGAGACCATGAAGGAGAAGGGCTTGCTGGGGGAGTTCCTGAGGACCCACAAGTATG
  ATCCTGCTAGGAAGTACCGCTTTGGTGACCTCAGCGTGACCTACGAGCCCATGGCCTACA
  TGGATGCTGCCTACTTTGGTGAGATCAGCATCGGGACTCCACCCCAGAACTTCCTGGTCC
  TTTTTGACACCGGCTCCTCCAACTTGTGGGTGCCCTCTGTCTACTGCCAGAGCCAGGCCT
  GCACCAGTCACTCCCGCTTCAACCCCGGCGAGTCGTCCACCTACTCCACCAATGGGCAGA
  CCTTCTCCCTGCAGTATGGCAGTGGCAGCCTCACCGGCTTCTTTGGCTATGACACCCCGA
  GCGGCCAAAACATCCAGGTCCCCAACCAGAATTTCGGCTTGATTGAAAATGAACCTGGTA
  CCAATTTGTCTATGCGCAATTTGAGTGCATATGGGCCGTGGCTACACCTGTCTGGCCGGG
  GAGAGGCCACCCAGCTTATACAGGGCATAGTGAACGGAGGCCCCCTAAACAAGCCCGGTT
  TAGAGCTTTCATGAACAACCCACGAGGGCTCCCCCGGGGGGGCCGATAGGCTTGGGCGAG
  TGGGTATACACCACTGTCACCGACCCCAATAAACGGAGCACATGTGTCAATACGTCTAGG
  TTGGCGGAATGCGTGGAGAAGAAAAAGCGAGGAAGGGAGCGATAACCGGGTAGTGTTAAC
  GAGCAATGATTCTACAGTTATGCTGCGAAGACAGCAAAGCCAGTAATCTGCGCCGAAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002630 unedited
  TGGGGAAGTGNNNTTTTGCATGCGTGTCACTTCCCGGCCAGGAGAGGCACTGNGGAGGGG
  TCAAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGA
  GTCGAGTTTTTTTTTTTTTTTTTTTAAAGAAGAACTATTTATTATTAGAGAAAGTCCAGA
  GTCCAGAAAAAGAAGGCTGAATCCAGAGTGGAAAGACAGATACAATGCCCTAGGAGGGTG
  CAGGGTCAAGAGGAAGAGGGGAGCCCACGTGTCGAGGCAGCAAGTCTAGGCGGCAGTGGC
  AAAGCCTACTCTGTTGTTGCCCAAGTCGTAGACGGAATAGTAGGACCTGAGGAAGACATC
  CCCGAGGATCCACAGGGGCTGGCCGTTCTGGGAGGACAGGTAGGTGGGCTCGACTCCCAC
  GGTGCAGTAGCCGTTGTTACTGAGGATATAGGAGGAAGGTGGCAGAGGGAACTCCACACC
  ATTGATGATGAAGGTCAAGCTGGGCAGATTCTGAATGCTGTTACAGTTCACGAGAAACTG
  TCCATACTCATCCTCCTGGGCCCCTGTGGCCTGCAGAAGAGCACTCATGTACTGGCTGGG
  CACAGTGAGCAAAAAGGTGCCTGTGTCCACGATGGCCTGGGCACCCTCAGAACACCAGCC
  CGAGGCCTGCCCGCCGATGAAGAACCTCTCAATGCAAATCTTGCATTNAAGTTCCTGGTG
  ACAGGGCCCAATAATACGTGCCGGGTCAGGCTGGTTTCCCACCCCCAAAGAAACCGGTTC
  CCGGTTGGACCCTGCTGTTGCGT
  
• Restriction Sites: Please inquire
• ACCN: NM_002630
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002630.1, NP_002621.1
• RefSeq Size: 1366 bp
• RefSeq ORF: 1167 bp
• Locus ID: 5225
• Cytogenetics: 6p21.1
• Protein Families: Protease, Secreted Protein
• Gene Summary: 'This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).