PDE4A (NM_006202) Human Untagged Clone

CAT#: SC124016

PDE4A (untagged)-Human phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE4A
• Synonyms: DPDE2; PDE4; PDE46
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006202, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCTTGGTGGATTTCTTCTGCGAGACCTGCTCTAAGCCTTGGCTGGTGGGCTGGTGGGACCAGTTCA
  AAAGGATGTTGAACCGTGAGCTCACACACCTGTCAGAAATGAGCAGGTCCGGAAACCAGGTCTCAGAGTA
  CATTTCCACAACATTCCTGGACAAACAGAATGAAGTGGAGATCCCATCACCCACGATGAAGGAACGAGAA
  AAACAGCAAGCGCCGCGACCAAGACCCTCCCAGCCGCCCCCGCCCCCTGTACCACACTTACAGCCCATGT
  CCCAAATCACAGGGTTGAAAAAGTTGATGCATAGTAACAGCCTGAACAACTCTAACATTCCCCGATTTGG
  GGTGAAGACCGATCAAGAAGAGCTCCTGGCCCAAGAACTGGAGAACCTGAACAAGTGGGGCCTGAACATC
  TTTTGCGTGTCGGATTACGCTGGAGGCCGCTCACTCACCTGCATCATGTACATGATATTCCAGGAGCGGG
  ACCTGCTGAAGAAATTCCGCATCCCTGTGGACACGATGGTGACATACATGCTGACGCTGGAGGATCACTA
  CCACGCTGACGTGGCCTACCATAACAGCCTGCACGCAGCTGACGTGCTGCAGTCCACCCACGTACTGCTG
  GCCACGCCTGCACTAGATGCAGTGTTCACGGACCTGGAGATTCTCGCCGCCCTCTTCGCGGCTGCCATCC
  ACGATGTGGATCACCCTGGGGTCTCCAACCAGTTCCTCATCAACACCAATTCGGAGCTGGCGCTCATGTA
  CAACGATGAGTCGGTGCTCGAGAATCACCACCTGGCCGTGGGCTTCAAGCTGCTGCAGGAGGACAACTGC
  GACATCTTCCAGAACCTCAGCAAGCGCCAGCGGCAGAGCCTACGCAAGATGGTCATCGACATGGTGCTGG
  CCACGGACATGTCCAAGCACATGACCCTCCTGGCTGACCTGAAGACCATGGTGGAGACCAAGAAAGTGAC
  CAGCTCAGGGGTCCTCCTGCTAGATAACTACTCCGACCGCATCCAGGTCCTCCGGAACATGGTGCACTGT
  GCCGACCTCAGCAACCCCACCAAGCCGCTGGAGCTGTACCGCCAGTGGACAGACCGCATCATGGCCGAGT
  TCTTCCAGCAGGGTGACCGAGAGCGCGAGCGTGGCATGGAAATCAGCCCCATGTGTGACAAGCACACTGC
  CTCCGTGGAGAAGTCTCAGGTGGGTTTTATTGACTACATTGTGCACCCATTGTGGGAGACCTGGGCGGAC
  CTTGTCCACCCAGATGCCCAGGAGATCTTGGACACTTTGGAGGACAACCGGGACTGGTACTACAGCGCCA
  TCCGGCAGAGCCCATCTCCGCCACCCGAGGAGGAGTCAAGGGGGCCAGGCCACCCACCCCTGCCTGACAA
  GTTCCAGTTTGAGCTGACGCTGGAGGAGGAAGAGGAGGAAGAAATATCAATGGCCCAGATACCGTGCACA
  GCCCAAGAGGCATTGACTGCGCAGGGATTGTCAGGAGTCGAGGAAGCTCTGGATGCAACCATAGCCTGGG
  AGGCATCCCCGGCCCAGGAGTCGTTGGAAGTTATGGCACAGGAAGCATCCCTGGAGGCCGAGCTGGAGGC
  AGTGTATTTGACACAGCAGGCACAGTCCACAGGCAGTGCACCTGTGGCTCCGGATGAGTTCTCGTCCCGG
  GAGGAATTCGTGGTTGCTGTAAGCCACAGCAGCCCCTCTGCCCTGGCTCTTCAAAGCCCCCTTCTCCCTG
  CTTGGAGGACCCTGTCTGTTTCAGAGCATGCCCCGGGCCTCCCGGGCCTCCCCTCCACGGCGGCCGAGGT
  GGAGGCCCAACGAGAGCACCAGGCTGCCAAGAGGGCTTGCAGTGCCTGCGCAGGGACATTTGGGGAGGAC
  ACATCCGCACTCCCAGCTCCTGGTGGCGGGGGGTCAGGTGGAGACCCTACCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006202 unedited
  TAATNCGGCACGAGGGCCATGTAACCAGGGCTGCTGCTGGGAGCGCGGAGGGGAAGGGAT
  CCCCCANCCCTGCTGNNCCNNNCCCAGCCCCTCCNCNCCTCCCCCTTCCACTACCCACCT
  GCCCNNCACCCCCTCCCCANTNNTTNTTAACCCCNNNACTCCCCAANCCCANCCTCTNTN
  TCCANCANCCCCANNCNNNCTAANTCTCCAACATCCCCTTNNTNNATTTCTTCTNCNAAA
  CCTNCTCTAANCCTTNNCTNNTNNNCTNNTNNNACCACTTCAAAANNATNTTNAACCNTN
  ACCTCACACACCTNTCAAAAATNACCANNTCCNNAAACCAANTCTCAAAATACATTTCCA
  CAACATTCCTNNACAAACAAAATNAANTNCACATCCCATCACCCACTATTAANNAACCAN
  AAAAACATCAACCNCCCCNACCAAAACCCTCCCATCCNCCCCCNCCCCCTNTACCACACT
  TACACCCCATTTCCCAAATCACANNNTTTAAAAATTTNATCCATAATAACACCCTNAACA
  ACCCTAACATTCCCCTATTTCCCCTAAATACCCATCAAAAAAACCTTCCNNCCCAAAAAC
  TNTAAAACCTTAACAACTTCTCCCTCCACATCTTTTTCCTTTCCTATTACACTTTATCCC
  CCTCATTTTACTTCCTCATCTACATCATATTCCCTNATCACAAACTCCTTAACAAATTTC
  CATCCCTTTTTACCCCATTTTNAAATACACACCTACCCTCNAATAACACTACCCCCTTAA
  CTTTCTCTACACTAAAACCCTTCAACATCCTCCCTTTTTATTACCCCAATTACTCTTANC
  TACTCTTCATTATATCCATTTTCTCTAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006202 unedited
  ACTTCAGGGCCAGGAGAGGCACTGGGGAGNGGTCACAGGGATGCCACCCGGGATCTGTTC
  AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTGGTTATCCTTT
  GTGTTTCTTTGTTTTTTTTTTCTTTTTTTGGTCTTTTTCTAAAAATGTTACAATTAAAAT
  TCAAAAAAATAAAATCCCTTCCGGGACCTCAGCCCCTTATTCCCCAGCCCCCACCTCCAA
  AAAAAGGCCCCGTGGGTGGGGGCAGGGGGAAAGGGGAAAAAAAGAAAACAGAATAAAACC
  CCGCTTTTCGTTTTCTTTTTTCTCAAGATGGACAAGAGGACAACTAGTCTTTGATGCACA
  TGACGAGGTGGTCTGGGGATCATGGGAGTGAGGGGATGAGTTCATGATATCCCGGCCTCC
  TGACCTGATTCCGAATTTCTGTATACCCGTGGTCACAATGCTGCCTAACGTGCTAGGATT
  GGTGGATCACTACCATGCTCACCTGTACTACCATGACAGCATGTAAGCTGCTGACGTGCT
  GCAATGTACTGATGTACTGCTGTGCACTACTTGCATAGATGCCGGGCCTGGAGATTTTTA
  GATTCTTGTTGGCGTCTGTCATGCTGCATTTGAATATGTGTATGATCTTGGCGTCTTAAT
  CATTTACTCATATTCACGACTGGGGATCATGTGCTAATGTGAGTCGATGCTTCAGTACTA
  CGACATGACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006202
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006202.1, NP_006193.1
• RefSeq Size: 4255 bp
• RefSeq ORF: 1944 bp
• Locus ID: 5141
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (4) contains an alternative 5' terminal exon and is missing several 5' coding exons compared to variant 1. It encodes a shorter isoform (4) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.