PDE4A (NM_006202) Human Untagged Clone

CAT#: SC124016

PDE4A (untagged)-Human phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 4


  "NM_006202" in other vectors (6)

Reconstitution Protocol

USD 760.00

2 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PDE4A
Synonyms DPDE2; PDE4; PDE46
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_006202, the custom clone sequence may differ by one or more nucleotides


ATGCCCTTGGTGGATTTCTTCTGCGAGACCTGCTCTAAGCCTTGGCTGGTGGGCTGGTGGGACCAGTTCA
AAAGGATGTTGAACCGTGAGCTCACACACCTGTCAGAAATGAGCAGGTCCGGAAACCAGGTCTCAGAGTA
CATTTCCACAACATTCCTGGACAAACAGAATGAAGTGGAGATCCCATCACCCACGATGAAGGAACGAGAA
AAACAGCAAGCGCCGCGACCAAGACCCTCCCAGCCGCCCCCGCCCCCTGTACCACACTTACAGCCCATGT
CCCAAATCACAGGGTTGAAAAAGTTGATGCATAGTAACAGCCTGAACAACTCTAACATTCCCCGATTTGG
GGTGAAGACCGATCAAGAAGAGCTCCTGGCCCAAGAACTGGAGAACCTGAACAAGTGGGGCCTGAACATC
TTTTGCGTGTCGGATTACGCTGGAGGCCGCTCACTCACCTGCATCATGTACATGATATTCCAGGAGCGGG
ACCTGCTGAAGAAATTCCGCATCCCTGTGGACACGATGGTGACATACATGCTGACGCTGGAGGATCACTA
CCACGCTGACGTGGCCTACCATAACAGCCTGCACGCAGCTGACGTGCTGCAGTCCACCCACGTACTGCTG
GCCACGCCTGCACTAGATGCAGTGTTCACGGACCTGGAGATTCTCGCCGCCCTCTTCGCGGCTGCCATCC
ACGATGTGGATCACCCTGGGGTCTCCAACCAGTTCCTCATCAACACCAATTCGGAGCTGGCGCTCATGTA
CAACGATGAGTCGGTGCTCGAGAATCACCACCTGGCCGTGGGCTTCAAGCTGCTGCAGGAGGACAACTGC
GACATCTTCCAGAACCTCAGCAAGCGCCAGCGGCAGAGCCTACGCAAGATGGTCATCGACATGGTGCTGG
CCACGGACATGTCCAAGCACATGACCCTCCTGGCTGACCTGAAGACCATGGTGGAGACCAAGAAAGTGAC
CAGCTCAGGGGTCCTCCTGCTAGATAACTACTCCGACCGCATCCAGGTCCTCCGGAACATGGTGCACTGT
GCCGACCTCAGCAACCCCACCAAGCCGCTGGAGCTGTACCGCCAGTGGACAGACCGCATCATGGCCGAGT
TCTTCCAGCAGGGTGACCGAGAGCGCGAGCGTGGCATGGAAATCAGCCCCATGTGTGACAAGCACACTGC
CTCCGTGGAGAAGTCTCAGGTGGGTTTTATTGACTACATTGTGCACCCATTGTGGGAGACCTGGGCGGAC
CTTGTCCACCCAGATGCCCAGGAGATCTTGGACACTTTGGAGGACAACCGGGACTGGTACTACAGCGCCA
TCCGGCAGAGCCCATCTCCGCCACCCGAGGAGGAGTCAAGGGGGCCAGGCCACCCACCCCTGCCTGACAA
GTTCCAGTTTGAGCTGACGCTGGAGGAGGAAGAGGAGGAAGAAATATCAATGGCCCAGATACCGTGCACA
GCCCAAGAGGCATTGACTGCGCAGGGATTGTCAGGAGTCGAGGAAGCTCTGGATGCAACCATAGCCTGGG
AGGCATCCCCGGCCCAGGAGTCGTTGGAAGTTATGGCACAGGAAGCATCCCTGGAGGCCGAGCTGGAGGC
AGTGTATTTGACACAGCAGGCACAGTCCACAGGCAGTGCACCTGTGGCTCCGGATGAGTTCTCGTCCCGG
GAGGAATTCGTGGTTGCTGTAAGCCACAGCAGCCCCTCTGCCCTGGCTCTTCAAAGCCCCCTTCTCCCTG
CTTGGAGGACCCTGTCTGTTTCAGAGCATGCCCCGGGCCTCCCGGGCCTCCCCTCCACGGCGGCCGAGGT
GGAGGCCCAACGAGAGCACCAGGCTGCCAAGAGGGCTTGCAGTGCCTGCGCAGGGACATTTGGGGAGGAC
ACATCCGCACTCCCAGCTCCTGGTGGCGGGGGGTCAGGTGGAGACCCTACCTGA


>OriGene 5' read for NM_006202 unedited
TAATNCGGCACGAGGGCCATGTAACCAGGGCTGCTGCTGGGAGCGCGGAGGGGAAGGGAT
CCCCCANCCCTGCTGNNCCNNNCCCAGCCCCTCCNCNCCTCCCCCTTCCACTACCCACCT
GCCCNNCACCCCCTCCCCANTNNTTNTTAACCCCNNNACTCCCCAANCCCANCCTCTNTN
TCCANCANCCCCANNCNNNCTAANTCTCCAACATCCCCTTNNTNNATTTCTTCTNCNAAA
CCTNCTCTAANCCTTNNCTNNTNNNCTNNTNNNACCACTTCAAAANNATNTTNAACCNTN
ACCTCACACACCTNTCAAAAATNACCANNTCCNNAAACCAANTCTCAAAATACATTTCCA
CAACATTCCTNNACAAACAAAATNAANTNCACATCCCATCACCCACTATTAANNAACCAN
AAAAACATCAACCNCCCCNACCAAAACCCTCCCATCCNCCCCCNCCCCCTNTACCACACT
TACACCCCATTTCCCAAATCACANNNTTTAAAAATTTNATCCATAATAACACCCTNAACA
ACCCTAACATTCCCCTATTTCCCCTAAATACCCATCAAAAAAACCTTCCNNCCCAAAAAC
TNTAAAACCTTAACAACTTCTCCCTCCACATCTTTTTCCTTTCCTATTACACTTTATCCC
CCTCATTTTACTTCCTCATCTACATCATATTCCCTNATCACAAACTCCTTAACAAATTTC
CATCCCTTTTTACCCCATTTTNAAATACACACCTACCCTCNAATAACACTACCCCCTTAA
CTTTCTCTACACTAAAACCCTTCAACATCCTCCCTTTTTATTACCCCAATTACTCTTANC
TACTCTTCATTATATCCATTTTCTCTAAT
>OriGene 3' read for NM_006202 unedited
ACTTCAGGGCCAGGAGAGGCACTGGGGAGNGGTCACAGGGATGCCACCCGGGATCTGTTC
AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTGGTTATCCTTT
GTGTTTCTTTGTTTTTTTTTTCTTTTTTTGGTCTTTTTCTAAAAATGTTACAATTAAAAT
TCAAAAAAATAAAATCCCTTCCGGGACCTCAGCCCCTTATTCCCCAGCCCCCACCTCCAA
AAAAAGGCCCCGTGGGTGGGGGCAGGGGGAAAGGGGAAAAAAAGAAAACAGAATAAAACC
CCGCTTTTCGTTTTCTTTTTTCTCAAGATGGACAAGAGGACAACTAGTCTTTGATGCACA
TGACGAGGTGGTCTGGGGATCATGGGAGTGAGGGGATGAGTTCATGATATCCCGGCCTCC
TGACCTGATTCCGAATTTCTGTATACCCGTGGTCACAATGCTGCCTAACGTGCTAGGATT
GGTGGATCACTACCATGCTCACCTGTACTACCATGACAGCATGTAAGCTGCTGACGTGCT
GCAATGTACTGATGTACTGCTGTGCACTACTTGCATAGATGCCGGGCCTGGAGATTTTTA
GATTCTTGTTGGCGTCTGTCATGCTGCATTTGAATATGTGTATGATCTTGGCGTCTTAAT
CATTTACTCATATTCACGACTGGGGATCATGTGCTAATGTGAGTCGATGCTTCAGTACTA
CGACATGACN
Restriction Sites NotI-NotI     
ACCN NM_006202
Insert Size 2700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006202.1, NP_006193.1
RefSeq Size 4255 bp
RefSeq ORF 1944 bp
Locus ID 5141
Cytogenetics 19p13.2
Protein Families Druggable Genome
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
Gene Summary 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]'
Transcript Variant: This variant (4) contains an alternative 5' terminal exon and is missing several 5' coding exons compared to variant 1. It encodes a shorter isoform (4) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.