GATA4 (NM_002052) Human Untagged Clone

CAT#: SC124037

GATA4 (untagged)-Human GATA binding protein 4 (GATA4)


  "NM_002052" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GATA4
Synonyms ASD2; TACHD; TOF; VSD1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC124037 sequence for NM_002052 edited (data generated by NextGen Sequencing)
ATGTATCAGAGCTTGGCCATGGCCGCCAACCACGGGCCGCCCCCCGGTGCCTACGAGGCGGGCGGCCCCG
GCGCCTTCATGCACGGCGCGGGCGCCGCGTCCTCGCCAGTCTACGTGCCCACACCGCGGGTGCCCTCCTC
CGTGCTGGGCCTGTCCTACCTCCAGGGCGGAGGCGCGGGCTCTGCGTCCGGAGGCGCCTCGGGCGGCAGC
TCCGGTGGGGCCGCGTCTGGTGCGGGGCCCGGGACCCAGCAGGGCAGCCCGGGATGGAGCCAGGCGGGAG
CCGACGGAGCCGCTTACACCCCGCCGCCGGTGTCGCCGCGCTTCTCCTTCCCGGGGACCACCGGGTCCCT
GGCGGCCGCCGCCGCCGCTGCCGCGGCCCGGGAAGCTGCGGCCTACAGCAGTGGCGGCGGAGCGGCGGGT
GCGGGCCTGGCGGGCCGCGAGCAGTACGGGCGCGCCGGCTTCGCGGGCTCCTACTCCAGCCCCTACCCGG
CTTACATGGCCGACGTGGGCGCGTCCTGGGCCGCAGCCGCCGCCGCCTCCGCCGGCCCCTTCGACAGCCC
GGTCCTGCACAGCCTGCCCGGCCGGGCCAACCCGGCCGCCCGACACCCCAATCTCGATATGTTTGACGAC
TTCTCAGAAGGCAGAGAGTGTGTCAACTGTGGGGCTATGTCCACCCCGCTCTGGAGGCGAGATGGGACGG
GTCACTATCTGTGCAACGCCTGCGGCCTCTACCACAAGATGAACGGCATCAACCGGCCGCTCATCAAGCC
TCAGCGCCGGCTGTCCGCCTCCCGCCGAGTGGGCCTCTCCTGTGCCAACTGCCAGACCACCACCACCACG
CTGTGGCGCCGCAATGCGGAGGGCGAGCCTGTGTGCAATGCCTGCGGCCTCTACATGAAGCTCCACGGGG
TCCCCAGGCCTCTTGCAATGCGGAAAGAGGGGATCCAAACCAGAAAACGGAAGCCCAAGAACCTGAATAA
ATCTAAGACACCAGCAGCTCCTTCAGGCAGTGAGAGCCTTCCTCCCGCCAGCGGTGCTTCCAGCAACTCC
AGCAACGCCACCACCAGCAGCAGCGAGGAGATGCGTCCCATCAAGACGGAGCCTGGTCTGTCATCTCACT
ACGGGCACAGCAGCTCCGTGTCCCAGACGTTCTCAGTCAGTGCGATGTCTGGCCATGGGCCCTCCATCCA
CCCTGTCCTCTCGGCCCTGAAGCTCTCCCCACAAGGCTATGCGTCTCCCGTCAGCCAGTCTCCACAGACC
AGCTCCAAGCAGGACTCTTGGAACAGCCTGGTCTTGGCCGACAGTCACGGGGACATAATCACTGCGTAA

Clone variation with respect to NM_002052.3
353 c=>n;354 g=>n;355 g=>n;356 c=>n;357 c=>n;358 g=>n;359 c=>n;360 c=>n;361 g=>n;1107 c=>t
>OriGene 5' read for NM_002052 unedited
GGTCAGATTTGTATACGACTCATATAGGCGGCCGCGAATTCGCACGAGCGCAGGGCCGCG
AGAGGCTTCGTCGCCGCTGCAGCTCCGGGGGCTCCCAGGGGAGCGTGCGCGGAACCTCCA
GGCCCAGCAGGACCCCGGCTGCGGCGAGGAGGAAGGAGCCAGCCTAGCAGCTTCTGCGCC
TGTGGCCGCGGGTGTCCTGGAGGCCTCTCGGTGTGACGAGTGGGGGACCCGAAGGCTCGT
GCGCCACCTCCAGGCCTGGACGCTGCCCTCCGTCTTCTGCCCCCAATAGGTGCGCCGGAC
CTTCAGGCCCTGGGGTGAATTCAGCTGCTCCTACATCAGCTTCCGGAACCACCAAAAATT
CAAATTGGGATTTTCCGGAGTAAACAAGAGCCTAGAGCCCTTTGCTCAATGCTGGATTTA
ATACGTATATATTTTTAAGCGAGTTGGTTTTTTCCCCTTTGATTTTTGATCTTCGCGACA
GTTCCTCCCACGCATATTATCGTTGTTGCCGTCGTTTTCTCTCCCCGCGTGGCTCCTTGA
CCTGCGAGGGAGAGAGAGGACACCGAAGCCGGGAGCTCGCAGGGACCATGTATCAGAGCT
TGGCCATGGCCGCCAACCACGGGCCGCCCCCCGGTGCCTACGAGGCGGGCGGCCCCGGCG
CCTTCATGCACGGCGCGGGCGCCGCGTCCTCGCCAGTCTACGTGCCCACACCGCGGGGTG
CCCTCCTCCGTGCTGGGCCTGTCCTACCTCCAGGGCGGAGGCGCGGGCTCTGCGTCCGGG
AGCGCCCTCGGCGGCAGCTCCGGGTGGGGCCCGGTCTGTTGCCGGGCCCGGNACCCCACA
GGGCAGCCCGGGGAGGACCCAGCGCGGGGCCAACGAGCCGCTTACCCCCCGCCGCCGTGT
CTCCCGCCCTTTTCCTCG
>OriGene 3' read for NM_002052 unedited
GCATGGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG
ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTGT
TTTTTAAAGAGGAATACAGATAAATTTATTAGTTAAATACTGATTTTCCAGCCATTTCAC
CTTAAGACAATGTTAACAGGTTTGTGGGTTAGGGAGGGTATACGAGGGGGCCTTTGGAAG
AAAACAATGTAAATGATGATTAAAACAGAATCTTGGTTCAAAGGTATTCTCTGCTACAGC
CAGTAGGATTTTGGAGTGAGGGGTCTGGGCGTGTGGGGAGGCGTAGTAATGCCACAGTCA
GCTACAGCTCTGCTGAGAAAGAGGAAGGAGTCTCCATGAGCTCCAGCATCAGGGGCAGAA
ACAGCAATGTGCAGAGGAGAACGCGGCAGATCCCGGAGCAGCTCAGAGTCGGAGGCTCCC
TCCAGGCCCCCCTGCCGTGTCTTAGCAGTCGTCTTCTTTCCAGGAATTCTGTTGTGTTGT
GTTTGTTTGCTTCGAATTCGTGTTGCAGAATCTCTGGCTTTTTGCCTCCTGGACAAAAGA
CTTCTGCCGGGGCGAAGCTGCAAGTCCTTGAGGACTCTGGCTTGCATCTTGACCAAGGGT
AGGAGGACCAACAGACCACAGGTGGGCCACCTTGGGACACTCTGCCCCTGACAGGAATTT
GTCACTTACCTCTCCCAGAAGACCTCAGGCTGGCTGATGCTGCCAATGCTTGACACAAAC
GTTTGTTTTCTAAACCTTTATTTGACCGTGACAAGGCTCATGACTCAGGGGTCGTTGTAA
TCCAACCTTGTTCAGGAAAAATTCAACG
Restriction Sites Please inquire     
ACCN NM_002052
Insert Size 3600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002052.2, NP_002043.2
RefSeq Size 3372 bp
RefSeq ORF 1329 bp
Locus ID 2626
Cytogenetics 8p23.1
Protein Families Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
Gene Summary 'This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]'
Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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