PAK4 (NM_005884) Human Untagged Clone

CAT#: SC124043

PAK4 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAK4
• Synonyms: p21 protein (Cdc42/Rac)-activated kinase 4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124043 sequence for NM_005884 edited (data generated by NextGen Sequencing)
  ATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCACCGC
  GTGCACACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTGGCAG
  AGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCGTCGACCCCGCCTGCATCACC
  TCCATCCAGCCCGGGGCCCCCAAGACCATCGTGCGGGGCAGCAAAGGTGCCAAAGATGGG
  GCCCTCACGCTGCTGCTGGACGAGTTTGAGAACATGTCGGTGACACGCTCCAACTCCCTG
  CGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAGGAAAATGGGATGCCAGAGGAG
  CCGGCCACCACGGCCAGAGGGGGCCCAGGGAAGGCAGGCAGCCGAGGCCGGTTCGCCGGT
  CACAGCGAGGCGGGTGGCGGCAGTGGTGACAGGCGACGGGCGGGGCCAGAGAAGAGGCCC
  AAGTCTTCCAGGGAGGGCTCAGGGGGTCCCCAGGAGTCCTCCCGGGACAAACGCCCCCTC
  TCCGGGCCTGATGTCGGCACCCCCCAGCCTGCTGGTCTGGCCAGTGGGGCGAAACTGGCA
  GCTGGCCGGCCCTTTAACACCTACCCGAGGGCTGACACGGACCACCCATCCCGGGGTGCC
  CAGGGGGAGCCTCATGACGTGGCCCCTAACGGGCCATCAGCGGGGGGCCTGGCCATCCCC
  CAGTCCTCCTCCTCCTCCTCCCGGCCTCCCACCCGAGCCCGAGGTGCCCCCAGCCCTGGA
  GTGCTGGGACCCCACGCCTCAGAGCCCCAGCTGGCCCCTCCAGCCTGCACCCCCGCCGCC
  CCTGCTGTTCCTGGGCCCCCTGGCCCCCGCTCACCACAGCGGGAGCCACAGCGAGTATCC
  CATGAGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGACCCAGGCGACCCCCGCTCCTAC
  CTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGGGCATCGTGTGCATCGCCACCGTG
  CGCAGCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGGACCTGCGCAAGCAGCAGAGGCGC
  GAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACTACCAGCACGAGAATGTGGTGGAG
  ATGTACAACAGCTACCTGGTGGGGGACGAGCTCTGGGTGGTCATGGAGTTCCTGGAAGGA
  GGCGCCCTCACCGACATCGTCACCCACACCAGGATGAACGAGGAGCAGATCGCGGCCGTG
  TGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCCAGGGCGTCATCCACCGGGAC
  ATCAAGAGCGACTCGATCCTGCTGACCCATGATGGCAGGGTGAAGCTGTCAGACTTTGGG
  TTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGAAGTCGCTGGTCGGCACGCCCTAC
  TGGATGGCCCCAGAGCTCATCTCCCGCCTTCCCTACGGGCCAGAGGTAGACATCTGGTCG
  CTGGGGATAATGGTGATTGAGATGGTGGACGGAGAGCCCCCCTACTTCAACGAGCCACCC
  CTCAAAGCCATGAAGATGATTCGGGACAACCTGCCACCCCGACTGAAGAACCTGCACAAG
  GTGTCGCCATCCCTGAAGGGCTTCCTGGACCGCCTGCTGGTGCGAGACCCTGCCCAGCGG
  GCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGGCCAAGGCAGGGCCGCCTGCCAGC
  ATCGTGCCCCTCATGCGCCAGAACCGCACCAGATGA
  
  Clone variation with respect to NM_005884.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005884 unedited
  NGTAACGTTCAGCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGT
  GTGGGGATTCAACATGGCGGCGGGAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAGG
  GAGGCGCGAGGGCGCGGAGTTCCAGGTCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCG
  AGCCGGCCGCACCGAGTCCCCGGCACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATC
  TCCGCGCCGTCCAACTTCGAGCACCGCGTGCACACGGGCTTCGACCAGCACGAGCAGAAG
  TTCACGGGGCTGCCCCGCCAGTGGCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAG
  CCCCTCGTCGACCCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCAAGACCATCGTG
  CGGGGCAGCAAAGGTGCCAAAGATGGGGCCCTCACGCTGCTGCTGGACGAGTTTGAGAAC
  ATGTCGGTGACACGCTCCAACTCCCTGCGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCC
  CGCCAGGAAAATGGGATGCCAGAGGAGCCGGCCACCACGGCCAGAGGGGGCCCAGGGAAG
  GCAGGCAGCCGAGGCCGGTTCGCCGGTCACAGCGAGCCGGGTTGGCCGGCAGTGGTTGAC
  ATGCCGACGGGCCGGGGCCACGATAATAGCCCCAAGTTCTTCCAGGGGAGGGCTCAGGGT
  GGTCCCCCAGGAATCCCTCCCGGGACAAACCCCCCCCTTCTCCCGGCCTGGATGTTCGGC
  CCCCCCACAACCCGCCTGATCTGGCGCCAGTGGGGCTTAAACTGANAACCTGGGCTGGCC
  CTTTGAAAACCTTCCCCGAGGTGCTGACCACGGTCCACCCTTATCCGGGGGGCGCCCTGT
  GGTGTTCTTTTATTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005884 unedited
  GCATTCAACTTCCCGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
  TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGCCCCCCTTTTTTTTTGGGT
  TTTTTTTTTTTTTTTTTACAAGACAATGTTTTAATTGTAAAACTAACTCGAGGCAGGGGT
  GGGCGGGCTGGGGCTGCGCTGACCGGGCAGGAACCTGGTTCTTCAGGCAGTGGTTCTGCC
  AGGGCCACCCCGCAGGACAGGGACCATCTGTCCCCCAATAAGGGCAGGGGCTAGAGTGTT
  ATAAAATGACAATATAAATAGACTTCTAGAAAAGAGGAGGCTGTCCAGGTGCAGTAGTCA
  TTTGCTGCAGGGGCTGGAGAGGGTGGGGGGCCCTGACCTGTCTCCCCATCCAGCCACAGA
  AAGGGGGCGCAGGGCTACGGGAAAAGGCCACTCTTCGGACATTCATGATCGACCCCCACA
  ATGGCTCANGGGGAGAGGGGGCTGGGTGTCANGCAAGGCGCCAGACACCCCTTGCGGGCT
  GGAGGACGGGGTGGCTGGACCTTTGCACACACACACACATGCACACTCACACGCGTGCAC
  ACACTCATACATGTTCACCTCTTAGTGTTCTCTCCACCCCCAGCGACCCCCACTGCAAAA
  CTTCCAGTGATGGGAGGGGCCGCTGCCCTTTCCTGGAGAACAGAAAAACATGGGGGGAAG
  GCCTTGTCCCCAAAAGGGTGGGGGTTCTCGGGAACCCTCGCTTGTGTTCAACCAAGTTCC
  TGGGGTTTTTTCTAAAAAATCCACCATATTCAAAAACTGGGGTTTCAATAATGAGGGGTC
  TCAATCTCCCCCACAAAGGAGGGTGGGTGCCACGCCGGAAATTCTTCCGGGCTTGGAGAA
  ATTGGAAGCCTGTCCCCCTATATGGCTTTAATGGGGCGGGGAGACCCCGGGGCTTT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005884
• ORF Size: 1776 bp
• Insert Size: 3000
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_005884.3, NP_005875.1
• RefSeq Size: 2838
• RefSeq ORF: 1776
• Locus ID: 10298
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Gene Summary: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (1).