Brk (PTK6) (NM_005975) Human Untagged Clone

CAT#: SC124087

PTK6 (untagged)-Human PTK6 protein tyrosine kinase 6 (PTK6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTK6
• Synonyms: BRK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005975 edited
  GCGGCCGCGAATTCGGCACGAGGTGGGCCTGAGGACTTGTGGGGCCGGCTGGGCCCTCAG
  GAGGAGCCGGGGTGCTCTGGAGCGCCTGTGGGTGCTGGGTGTGGCTGCTTCCCGGCCTGT
  CATGAGGAAGTGGGACGGCCCGCCTGCCACGCCCAGCTCTGGGTGCTCCAGCTGGGCCAC
  AGCCTGGTCCTGCCGCTGCGCCCGCCCGCCATGGTGTCCCGGGACCAGGCTCACCTGGGC
  CCCAAGTATGTGGGCCTCTGGGACTTCAAGTCCCGGACGGACGAGGAGCTGAGCTTCCGC
  GCGGGGGACGTCTTCCACGTGGCCAGGAAGGAGGAGCAGTGGTGGTGGGCCACGCTGCTG
  GACGAGGCGGGTGGGGCCGTGGCCCAGGGCTATGTGCCCCACAACTACCTGGCCGAGAGG
  GAGACGGTGGAGTCGGAACCGTGGTTCTTTGGCTGCATCTCCCGCTCGGAAGCTGTGCGT
  CGGCTGCAGGCCGAGGGCAACGCCACGGGCGCCTTCCTGATCAGGGTCAGCGAGAAGCCG
  AGTGCCGACTACGTCCTGTCGGTGCGGGACACGCAGGCTGTGCGGCACTACAAGATCTGG
  CGGCGTGCCGGGGGCCGGCTGCACCTGAACGAGGCGGTGTCCTTCCTCAGCCTGCCCGAG
  CTTGTGAACTACCACAGGGCCCAGAGCCTGTCCCACGGCCTGCGGCTGGCCGCGCCCTGC
  CGGAAGCACGAGCCTGAGCCCCTGCCCCATTGGGATGACTGGGAGAGGCCGAGGGAGGAG
  TTCACGCTCTGCAGGAAGCTGGGGTCCGGCTACTTTGGGGAGGTCTTCGAGGGGCTCTGG
  AAAGACCGGGTCCAGGTGGCCATTAAGGTGATTTCTCGAGACAACCTCCTGCACCAGCAG
  ATGCTGCAGTCGGAGATCCAGGCCATGAAGAAGCTGCGGCACAAACACATCCTGGCGCTG
  TACGCCGTGGTGTCCGTGGGGGACCCCGTGTACATCATCACGGAGCTCATGGCCAAGGGC
  AGCCTGCTGGAGCTGCTCCGCGACTCTGATGAGAAAGTCCTGCCCGTTTCGGAGCTGCTG
  GACATCGCCTGGCAGGTGGCTGAGGGCATGTGTTACCTGGAGTCGCAGAATTACATCCAC
  CGGGACCTGGCCGCCAGGAACATCCTCGTCGGGGAAAACACCCTCTGCAAAGTTGGGGAC
  TTCGGGTTAGCCAGGCTTATCAAGGAGGACGTCTACCTCTCCCATGACCACAATATCCCC
  TACAAGTGGACGGCCCCTGAAGCGCTCTCCCGAGGCCATTACTCCACCAAATCCGACGTC
  TGGTCCTTTGGGATTCTCCTGCATGAGATGTTCAGCAGGGGTCAGGTGCCCTACCCAGGC
  ATGTCCAACCATGAGGCCTTCCTGAGGGTGGACGCCGGCTACCGCATGCCCTGCCCTCTG
  GAGTGCCCGCCCAGCGTGCACAAGCTGATGCTGACATGCTGGTGCAGGGACCCCGAGCAG
  AGACCCTGCTTCAAGGCCCTGCGGGAGAGGCTCTCCAGCTTCACCAGCTACGAGAACCCG
  ACCTGAGCTGCTGTGGAGCGGGCATGGCCGGGCCCTGCTGAGGAGGGGCCTGGGCAGAGG
  GCCTGGACCTGGGATCAAGGCCCACGCGCTTCCCTGGGGTTTACTGAGGTGATGGGTGCA
  GGAAAGGTTCACAAATGTGGAGTGTCTGCGTCCAATACACGCGTGTGCTCCTCTCCTTAC
  TCCATCGTGTGTGCCTTGGGTCTCAGCTGCTGACACGCAGCCTGCTCTGGAGCCTGCAGA
  TGAGATCCGGAGACTGACACGAAGCCAGCAGAGGTCAGAGGGGACTCTGACCACAGCCCG
  CTCTCTGGCTGTCTGTCTGCAGTGCCCGGCTGAGGGTGGGAGGCAAACACGCCTTGTTCC
  TGCTCTTCCCAGTTCAGCTTGGTGGGAAGAAAGTCATTTCGCGTGGCTCGGGACGCTCAT
  GTAAATTTGGTTTTGGTGCTCAAGGGTTCTTTCCTCCCAGGGGCAGGTGTTTCTTTCCTG
  TTTGTCTTGTGTCTTGAGAGCTTGGCCTTATGACCAGTGAGAACTCTCTCCCTGGTCTCT
  GCCAGCCCAAGCATCACTGCCCGAGGCGCCAGCTCAGTTTCACCGTTCCACGTCCACAAG
  GGGCTTTTCCCACCTTCACCTTTGTCGCTGGGGTCAGTGCTGGGAAAGCGCCCCTTCACT
  TCCTGCGCTGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005975 unedited
  CAGTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGGGCCTG
  AGGACTTGTGGGGCCGGCTGGGCCCTCAGGAGGAGCCGGGGTGCTCTGGAGCGCCTGTGG
  GTGCTGGGTGTGGCTGCTTCCCGGCCTGTCATGAGGAAGTGGGACGGCCCGCCTGCCACG
  CCCAGCTCTGGGTGCTCCAGCTGGGCCACAGCCTGGTCCTGCCGCTGCGCCCGCCCGCCA
  TGGTGTCCCGGGACCAGGCTCACCTGGGCCCCAAGTATGTGGGCCTCTGGGACTTCAAGT
  CCCGGACGGACGAGGAGCTGAGCTTCCGCGCGGGGGACGTCTTCCACGTGGCCAGGAAGG
  AGGAGCAGTGGTGGTGGGCCACGCTGCTGGACGAGGCGGGTGGGGCCGTGGCCCAGGGCT
  ATGTGCCCCACAACTACCTGGCCGAGAGGGAGACGGTGGAGTCGGAACCGTGGTTCTTTG
  GCTGCATCTCCCGCTCGGAAGCTGTGCGTCGGCTGCAGGCCGAGGGCAACGCCACGGGCG
  CCTTCCTGATCAGGGTCAGCGAGAAGCCGAGTGCCGACTACGTCCTGTCGGTGCGGGACA
  CGCAGGCTGTGCGGCACNTACAGATCTGGCGGCGTGCCGGGGGCCGGCTGCACCTGAACG
  AGGCGGTGTCCTTCCTCACCCTGCCCGAGCTTGTGAACTACCACAGGGCCCAGAGCCTGT
  CCCACGGGCTGCGGCTGGGCCGCGCCCTGGCCGGAGCACAAACCTGAACCCCTGCCCCAT
  TTGGAAGACTGGGAAAAGGCCCAGGAAGGATTTAACCCCTCGAGGAAACCTGGGTCCCGC
  CTACTTTGGGGAGTTCTTCAAGGGGCTTTGGAAAGACCCGGTCCGGTGGCCTTAAAGGTG
  AATTTTGAGAAAACCTCCTGCCCACCAAAAGTGTGATTCGGAAATCAGCCCAAAT
  
• Restriction Sites: Please inquire
• ACCN: NM_005975
• Insert Size: 3200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005975.2, NP_005966.1
• RefSeq Size: 2519 bp
• RefSeq ORF: 1356 bp
• Locus ID: 5753
• Cytogenetics: 20q13.33
• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.