DNase gamma (DNASE1L3) (NM_004944) Human Untagged Clone

CAT#: SC124096

DNASE1L3 (untagged)-Human deoxyribonuclease I-like 3 (DNASE1L3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNASE1L3
• Synonyms: DHP2; DNAS1L3; LSD; SLEB16
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004944, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCACGGGAGCTGGCCCCACTGCTGCTTCTCCTCCTCTCCATCCACAGCGCCCTGGCCATGAGGATCT
  GCTCCTTCAACGTCAGGTCCTTTGGGGAAAGCAAGCAGGAAGACAAGAATGCCATGGATGTCATTGTGAA
  GGTCATCAAACGCTGTGACATCATACTCGTGATGGAAATCAAGGACAGCAACAACAGGATCTGCCCCATA
  CTGATGGAGAAGCTGAACAGAAATTCAAGGAGAGGCATAACGTACAACTATGTGATTAGCTCTCGGCTTG
  GAAGAAACACATATAAAGAACAATATGCCTTTCTCTACAAGGAAAAGCTGGTGTCTGTGAAGAGGAGTTA
  TCACTACCATGACTATCAGGATGGAGACGCAGATGTGTTTTCCAGGGAGCCCTTTGTGGTCTGGTTCCAA
  TCTCCCCACACTGCTGTCAAAGACTTCGTGATTATCCCCCTGCACACCACCCCAGAGACATCCGTTAAGG
  AGATCGATGAGTTGGTTGAGGTCTACACGGACGTGAAACACCGCTGGAAGGCGGAGAATTTCATTTTCAT
  GGGTGACTTCAATGCCGGCTGCAGCTACGTCCCCAAGAAGGCCTGGAAGAACATCCGCTTGAGGACTGAC
  CCCAGGTTTGTTTGGCTGATCGGGGACCAAGAGGACACCACGGTGAAGAAGAGCACCAACTGTGCATATG
  ACAGGATTGTGCTTAGAGGACAAGAAATCGTCAGTTCTGTTGTTCCCAAGTCAAACAGTGTTTTTGACTT
  CCAGAAAGCTTACAAGCTGACTGAAGAGGAGGCCCTGGATGTCAGCGACCACTTTCCAGTTGAATTTAAA
  CTACAGTCTTCAAGGGCCTTCACCAACAGCAAAAAATCTGTCACTCTAAGGAAGAAAACAAAGAGCAAAC
  GCTCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004944 unedited
  TCTATAGTGGGGCCAGACAATCCGGCACGAGAAGTACTGCTGTCTTCTCACAGAGTCTTG
  AAGCCAGAGTATCGCCAGGATGTCACGGGAGCTGGCCCCAGGGCTGCTTTTCCTGCTCTC
  CATCCACAGCGCCCTGGCCATGAGGATCTGCTCCTTCAACGTCAGGTCCTTTGGGGAAAG
  CAAGCAGGAAGACAAGAATGCCATGGATGTCATTGTGAAGGTCATCAAACGCTGTGACAT
  CATACTCGTGATGGAAATCAAGGACAGCAACAACAGGATCTGCCCCATACTGATGGAGAA
  GCTGAACAGAAATTCAAGGAGAGGCATAACATACAACTATGTGATTAGCTCTCGGCTTGG
  AAGAAACACATATAAAGAACAATATGCCTTTCTCTACAAGGAAAAGCTGGTGTCTGTGAA
  GAGGAGTTATCACTACCATGACTATCAGGATGGAGACGCAGATGTGTTTTCCAGGGAGCC
  CTTTGTGGTCTGGTTCCAATCTCCCCACACTGCTGTCAAAGACTTCGTGATTATCCCCCT
  GCACACCACCCCAGATACATCCGTTAAGGAGATCGATGAGTTGGTTGAGGTCTACACGGA
  CGTGAAACACCGCTTGAAGGCCGAGAATGTCATTTTCGTGGGTGACTTCCATGCCGGCTG
  CGGGCTACTTCCCAAAAAGGCCCTGAAGAAACATCCCTTTAGGACTGACCCCCAGTTTGT
  TCGGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004944 unedited
  CGGATTGACTAGGAGCGTCACAGGGATGCCCCCGGGATCTGTTAAGAAACAGCTATGACC
  TTGCCGCAATCTAGAGTCGAGTTTTTTTTTTGGGTTTTTATCTGTTAGAGACATTTTATT
  TAGAGGCAAGAAATGGTTAATAAGATGAGACCCTTGGGTCTAGGAGCGTTTGCTCTTTGT
  TTTCTTCCTTAGAGTGACAGATTTTTTGCTGTTGGTGAAGGCCCTTGAAGACTGTAGTTT
  AAATTCAACTGGAAAGTGGTCGCTGACATCCAGGGCCTCCTCTTCAGTCAGCTTGTAAGC
  TTTCTGGAAGTCAAAAACACTGTTTGACTTGGGAACAACAGAACTGACGATTTCTTGTCC
  TCTAAGCACAATCCTGTCATATGCACAGTTGGTGCTCTTCTTCACCGTGGTGTCCTCTTG
  GTCCCCGATCAGCCAAACAAACCTGGGGTCAGTCCTCAAGCGGATGTTCTTCCAGGCCTT
  CTTGGGGACGTAGCTGCAGCCGGCATTGAAGTCACCCATGAAAATGAAATTCTCCGCCTT
  CCAGCGGTGTTTCACGTCCGTGTAGACCTCAACCAACTCATCGATCTCCTTAACGGATGT
  CTCTGGNGTGGTGTGCAGGGGGATAATCACGAAGTCTTTGACAGCAGTGTGGGGAGATTG
  GAACCAGACCACAAAGGGCTCCCTGGAAAACACATCTGCGTCTCCATCCTGATAGTCA
  
• Restriction Sites: Please inquire
• ACCN: NM_004944
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004944.2, NP_004935.1
• RefSeq Size: 1463 bp
• RefSeq ORF: 918 bp
• Locus ID: 1776
• Cytogenetics: 3p14.3
• Domains: Exo_endo_phos, DNaseIc
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the deoxyribonuclease I family. The encoded protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Mutations in this gene are a cause of systemic lupus erythematosus-16. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).