KCNH2 (NM_172057) Human Untagged Clone

CAT#: SC124103

KCNH2 (untagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNH2
• Synonyms: ERG-1; ERG1; H-ERG; HERG; HERG1; Kv11.1; LQT2; SQT1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124103 sequence for NM_172057 edited (data generated by NextGen Sequencing)
  ATGGCGGCCCCAGCCGGGAAGGCGAGCAGGACAGGGGCTCTGCGGCCCAGGGCCCAGAAA
  GGCCGGGTGAGGCGGGCCGTGCGCATCTCCAGCCTCGTGGCCCAGGAGGTCCTGTCCCTG
  GGCGCCGACGTGCTGCCTGAGTACAAGCTGCAGGCACCGCGCATCCACCGCTGGACCATC
  CTGCATTACAGCCCCTTCAAGGCCGTGTGGGACTGGCTCATCCTGCTGCTGGTCATCTAC
  ACGGCTGTCTTCACACCCTACTCGGCTGCCTTCCTGCTGAAGGAGACGGAAGAAGGCCCG
  CCTGCTACCGAGTGTGGCTACGCCTGCCAGCCGCTGGCTGTGGTGGACCTCATCGTGGAC
  ATCATGTTCATTGTGGACATCCTCATCAACTTCCGCACCACCTACGTCAATGCCAACGAG
  GAGGTGGTCAGCCACCCCGGCCGCATTGCCGTCCACTACTTCAAGGGCTGGTTCCTCATC
  GACATGGTGGCCGCCATCCCCTTCGACCTGCTCATCTTTGGCTCTGGCTCTGAGGAGCTG
  ATCGGGCTGCTGAAGACTGCGCGGCTGCTGCGGCTGGTGCGCGTGGCGCGGAAGCTGGAT
  CGCTACTCAGAGTACGGCGCGGCCGTGCTGTTCTTGCTCATGTGCACCTTTGCGCTCATC
  GCGCACTGGCTGGCCTGCATCTGGTACGCCATCGGCAACATGGAGCAGCCACACATGGAC
  TCACGCATCGGCTGGCTGCACAACCTGGGCGACCAGATAGGCAAACCCTACAACAGCAGC
  GGCCTGGGCGGCCCCTCCATCAAGGACAAGTATGTGACGGCGCTCTACTTCACCTTCAGC
  AGCCTCACCAGTGTGGGCTTCGGCAACGTCTCTCCCAACACCAACTCAGAGAAGATCTTC
  TCCATCTGCGTCATGCTCATTGGCTCCCTCATGTACGCTAGCATCTTCGGCAACGTGTCG
  GCCATCATCCAGCGGCTGTACTCGGGCACAGCCCGCTACCACACACAGATGCTGCGGGTG
  CGGGAGTTCATCCGCTTCCACCAGATCCCCAATCCCCTGCGCCAGCGCCTCGAGGAGTAC
  TTCCAGCACGCCTGGTCCTACACCAACGGCATCGACATGAACGCGGTGCTGAAGGGCTTC
  CCTGAGTGCCTGCAGGCTGACATCTGCCTGCACCTGAACCGCTCACTGCTGCAGCACTGC
  AAACCCTTCCGAGGGGCCACCAAGGGCTGCCTTCGGGCCCTGGCCATGAAGTTCAAGACC
  ACACATGCACCGCCAGGGGACACACTGGTGCATGCTGGGGACCTGCTCACCGCCCTGTAC
  TTCATCTCCCGGGGCTCCATCGAGATCCTGCGGGGCGACGTCGTCGTGGCCATCCTGGGG
  AAGAATGACATCTTTGGGGAGCCTCTGAACCTGTATGCAAGGCCTGGCAAGTCGAACGGG
  GATGTGCGGGCCCTCACCTACTGTGACCTACACAAGATCCATCGGGACGACCTGCTGGAG
  GTGCTGGACATGTACCCTGAGTTCTCCGACCACTTCTGGTCCAGCCTGGAGATCACCTTC
  AACCTGCGAGATACCAACATGATCCCGGGCTCCCCCGGCAGTACGGAGTTAGAGGGTGGC
  TTCAGTCGGCAACGCAAGCGCAAGTTGTCCTTCCGCAGGCGCACGGACAAGGACACGGAG
  CAGCCAGGGGAGGTGTCGGCCTTGGGGCCGGGCCGGGCGGGGGCAGGGCCGAGTAGCCGG
  GGCCGGCCGGGGGGGCCGTGGGGGGAGAGCCCGTCCAGTGGCCCCTCCAGCCCTGAGAGC
  AGTGAGGATGAGGGCCCAGGCCGCAGCTCCAGCCCCCTCCGCCTGGTGCCCTTCTCCAGC
  CCCAGGCCCCCCGGAGAGCCGCCGGGTGGGGAGCCCCTGATGGAGGACTGCGAGAAGAGC
  AGCGACACTTGCAACCCCCTGTCAGGCGCCTTCTCAGGAGTGTCCAACATTTTCAGCTTC
  TGGGGGGACAGTCGGGGCCGCCAGTACCAGGAGCTCCCTCGATGCCCCGCCCCCACCCCC
  AGCCTCCTCAACATCCCCCTCTCCAGCCCGGGTCGGCGGCCCCGGGGCGACGTGGAGAGC
  AGGCTGGATGCCCTCCAGCGCCAGCTCAACAGGCTGGAGACCCGGCTGAGTGCAGACATG
  GCCACTGTCCTGCAGCTGCTACAGAGGCAGATGACGCTGGTCCCGCCCGCCTACAGTGCT
  GTGACCACCCCGGGGCCTGGCCCCACTTCCACATCCCCGCTGTTGCCCGTCAGCCCCCTC
  CCCACCCTCACCTTGGACTCGCTTTCTCAGGTTTCCCAGTTCATGGCGTGTGAGGAGCTG
  CCCCCGGGGGCCCCAGAGCTTCCCCAAGAAGGCCCCACACGACGCCTCTCCCTACCGGGC
  CAGCTGGGGGCCCTCACCTCCCAGCCCCTGCACAGACACGGCTCGGACCCGGGCAGTTAG
  
  Clone variation with respect to NM_172057.2
  447 c=>t;519 c=>t;672 a=>g;936 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172057 unedited
  GAGTCAGAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCACCCT
  GGCAGCAGGGCCCACGCCACGGGGCCATGGGCAGCTCGAGCCAGGCAGGCTGCTGCCCAC
  GCTTACTGCCAGGGTGACCCCAGCCCTGGGGCCCAGCCACAACCACCCTGGCTTCATGCC
  AGGGGCTGCTCTGGTTGCCAGTCGGCCAGCCTCGGGGGTGCAGCCTGGGCTGGGACTGCT
  GCTGGGGTGCAGGTGAGGCAGTGGCCGGGCCCTCAGGCCCCAGGGCAGGCAGGCTGCAGG
  GAGCCAAGTCCTCCATGGCGGCCCCAGCCGGGAAGGCGAGCAGGACAGGGGCTCTGCGGC
  CCAGGGCCCAGAAAGGCCGGGTGAGGCGGGCCGTGCGCATCTCCAGCCTCGTGGCCCAGG
  AGGTCCTGTCCCTGGGCGCCGACGTGCTGCCTGAGTACAAGCTGCAGGCACCGCGCATCC
  ACCGCTGGACCATCCTGCATTACAGCCCCTTCAAGGCCGTGTGGGACTGGCTCATCCTGC
  TGCTGGTCATCTACACGGCTGTCTTCACACCCTACTCGGCTGCCTTCCTGCTGAAGGAGA
  CGGAAGAAGGCCCGCCTGCTACCGAGTGTGGCTACGCCTGCCAGCCGCTGGCTGTGGTGG
  ACCTCATCGTGGACATCATGTTCATTGTGGACATCCTCATCAACTTCCGCACCACCTACG
  TCAATGCCAACGAGGAGGTGGTCAGCCAACCCGGCCGCATTGGCGTCCACTACTTCAGGG
  GCTGGTTTCTCATCGACATGGTGGCCGCCATCCCCTTCGAACTGCTTATCTTTGGCTCTG
  GGTCTTAAGACCTGATCGGGCCTGTGAAAAATGGCCCGCTGCTGCGGCTGGTGCCCCTGG
  CCCCGAAACTGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_172057 unedited
  AGTTTGCTGACAGGCAACCGCGGGGATGTGGAAGTGGGGCCAGGCCCCGGGGTGGTCACA
  GCTTTTTAGGCGGGCGGGACNNNNNAATGTGCCTCGGTAGCATCTGCAGGACAGTGGCCA
  TGTCTGCACTCAGCCGGGTCTCCAGCCTGTTGAGCTGGCGCTGGAGGGCATCCAGCCTGC
  TCTCCACGTCGCCCCGGGGCCGCCGACCCGGGCTGGAGAGGGGGATGTTGAGGAGGCTGG
  GGGTGGGGGCGGGGCATCGAGGGAGCTCCTGGTACTGGCGGCCCCGACTGTCCCCCCAGA
  AGCTGAAAATGTTGGACACTCCTGAGAAGGCGCCTGACAGGGGGTTGCAAGTGTCGCTGC
  TCTTCTCGCAGTCCTCCATCAGGGGCTCCCCACCCGGCGGCTCTCCGGGGGGCCTGGGGC
  TGGAGAAGGGCACCAGGCGGAGGGGGCTGGAGCTGCGGCCTGGGCCCTCATCCTCACTGC
  TCTCAGGGCTGGAGGGGCCACTGGACGGGCTCTCCCCCCACGGCCCCCCCGGCCGGCCCC
  GGCTACTCGGCCCTGGCCCCGCCCGGCCCGGCCCCAAGGCCGACACCCTCCCTGGGCTGC
  TTCCGTGTCCTTGGTCCGTGCGCCCTGCGGAAAGGACAAACTTGGCGCTTGGCGTTGCCG
  GACTGAAGCCCACCTCTTCTAACTCTCGTACCTGCCCGGGGGGAGACCCTCGGAATCATG
  GCTGGGTAATCCTCCGCAGGGTTTGCAAAGGCTGATCTTCCCAGGCCTGGGACTCAAGTA
  ATTGGGTTCCGAAGAAAATCTCG
  
• Restriction Sites: Please inquire
• ACCN: NM_172057
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172057.1, NP_742054.1
• RefSeq Size: 3191 bp
• RefSeq ORF: 2460 bp
• Locus ID: 3757
• Cytogenetics: 7q36.1
• Domains: cNMP, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transcription Factors, Transmembrane
• Gene Summary: 'This gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3), also known as HERG1b, differs in the 5' UTR and 5' coding region compared to variant 1, resulting in a shorter isoform (c) with a different N-terminus compared to isoform a.