MAD2 (MAD2L1) (NM_002358) Human Untagged Clone

CAT#: SC124109

MAD2L1 (untagged)-Human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAD2L1
• Synonyms: HSMAD2; MAD2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124109 sequence for NM_002358 edited (data generated by NextGen Sequencing)
  ATGGCGCTGCAGCTCTCCCGGGAGCAGGGAATCACCCTGCGCGGGAGCGCCGAAATCGTG
  GCCGAGTTCTTCTCATTCGGCATCAACAGCATTTTATATCAGCGTGGCATATATCCATCT
  GAAACCTTTACTCGAGTGCAGAAATACGGACTCACCTTGCTTGTAACTACTGATCTTGAG
  CTCATAAAATACCTAAATAATGTGGTGGAACAACTGAAAGATTGGTTATACAAGTGTTCA
  GTTCAGAAACTGGTTGTAGTTATCTCAAATATTGAAAGTGGTGAGGTCCTGGAAAGATGG
  CAGTTTGATATTGAGTGTGACAAGACTGCAAAAGATGACAGTGCACCCAGAGAAAAGTCT
  CAGAAAGCTATCCAGGATGAAATCCGTTCAGTGATCAGACAGATCACAGCTACGGTGACA
  TTTCTGCCACTGTTGGAAGTTTCTTGTTCATTTGATCTGCTGATTTATACAGACAAAGAT
  TTGGTTGTACCTGAAAAATGGGAAGAGTCGGGACCACAGTTTATTACCAATTCTGAGGAA
  GTCCGCCTTCGTTCATTTACTACTACAATCCACAAAGTAAATAGCATGGTGGCCTACAAA
  ATTCCTGTCAATGACTGA
  
  Clone variation with respect to NM_002358.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002358 unedited
  NCGCCCCGCNAATTCGGCACGAGGTTGGAGCCGCTGTGGTTGCTGTCCGCGGAGTGGAAG
  CGCGTGCTTTTGTTTGTGTCCCTGGCCATGGCGCTGCAGCTCTCCCGGGAGCAGGGAATC
  ACCCTGCGCGGGAGCGCCGAAATCGTGGCCGAGTTCTTCTCATTCGGCATCAACAGCATT
  TTATATCAGCGTGGCATATATCCATCTGAAACCTTTACTCGAGTGCAGAAATACGGACTC
  ACCTTGCTTGTAACTACTGATCTTGAGCTCATAAAATACCTAAATAATGTGGTGGAACAA
  CTGAAAGATTGGTTATACAAGTGTTCAGTTCAGAAACTGGTTGTAGTTATCTCANATATT
  GAAAGTGGTGAGGTCCTGGAAAGATGGCAGTTTGATATTGAGTGTGACAAGACTGCAAAA
  GATGACAGTGCACCCAGAGAAAAGTCTCAGAAAGCTATCCAGGATGAAATCCGTTCAGTG
  ATCAGACAGATCACAGCTACGGTGACATTTCTGCCACTGTTGGAAGTTTCTTGTTCATTT
  GATCTGCTGATTTATACAGACAAAGATTTGGTTGTANCCTGAAAATGGGAAGAGTCGGGA
  CCACAGTTTATTACCAATTCTGAGGAAGTCCGCCTTCGTTCATTTACTACTACAATCCAC
  ANAGTAAATAGCATGGTGGCCTACAAAATTCCTGTCATTGACTGAGGATGACATGAGGAA
  ANATATGTAATTGTAATTTTGAATGTGGTTNTCCGANATAGGTCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002358 unedited
  AGGGGNAATTAGCTGCAGTTGAAATTNGCGAGGCNNNNNNGCACTGGGGAGGGGTCACAG
  GGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCTTCCGCAATCTAGAGTCGA
  GTTTTTTTTTTTTTTTTTTTTAAGACAAATTTAAAACAAACTTAACTTTATTTCCTCACT
  TTCACTTAAAACTTGATTTTATAAAACACATGAAAAAACATTTTTAAGAGTTCTGTATCA
  CAGAACATTAAACAGTACAAATATCCATTGCTTCATAGGTTCAAGTTACATAAATTAAAG
  TCAAATAATTGGAAACTGATTCAATAGGGAAAACTATACATGAAATGAAGGTCAAAAGGA
  GCTATACAGCAATATTTCATTGTTTATAGATTATGAGTTACTTTCAGGACCTTAACAAAG
  ATTCTGAATATTTAGACTTCCTTTGTTGTATTTTATACTTAAATATCTCCCTACCTATAC
  TGAGTCAAACTACTTGACCAAAACATCTGATTTAGGAAAGCATCTAGCTTTATAGCACAA
  GTTTTTCCATCTACAGTTACTATCTTCAAAGGAAATATACATCACAATGGTTGACAAAAA
  ACCTCCTGGTTCCTTTTGAACAATGTGCAATANATTCATGATGTTAACTCCATGGTAAGT
  CAAATAGGTACCAAAAAAATAAAAGGAACAATTACACACAGTTCAGTAAGTATCATTTTG
  GTTTTCTCCATGTAAAAATTAACCAATGAAATANAACATATCAACTATAGATGACCTGAT
  TTCAGGAAAACCACATTTANAATTACATTTCN
  
• Restriction Sites: Please inquire
• ACCN: NM_002358
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002358.2, NP_002349.1
• RefSeq Size: 1390 bp
• RefSeq ORF: 618 bp
• Locus ID: 4085
• Cytogenetics: 4q27
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: 'MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]'