UBE2I (BC000744) Human Untagged Clone

CAT#: SC124121

(untagged)-Homo sapiens, Similar to ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), clone MGC:2377 IMAGE:2819732, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2I
• Synonyms: C358B7.1|P18|UBC9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC000744, the custom clone sequence may differ by one or more nucleotides
  
  
  CCGTGCTCTGCCTCCTGTGTTCCAGGGACTTTGAACATGTCGGGGATCGCCCTCAGCAGACTCGCCCAGG
  AGAGGAAAGCATGGAGGAAAGACCACCCATTTGGTTTCGTGGCTGTCCCAACAAAAAATCCCGATGGCAC
  GATGAACCTCATGAACTGGGAGTGCGCCATTCCAGGAAAGAAAGGGACTCCGTGGGAAGGAGGCTTGTTT
  AAACTACGGATGCTTTTCAAAGATGATTATCCATCTTCGCCACCAAAATGTAAATTCGAACCACCATTAT
  TTCACCCGAATGTGTACCCTTCGGGGACAGTGTGCCTGTCCATCTTAGAGGAGGACAAGGACTGGAGGCC
  AGCCATCACAATCAAACAGATCCTATTAGGAATACAGGAACTTCTAAATGAACCAAATATCCAAGACCCA
  GCTCAAGCAGAGGCCTACACGATTTACTGGTTAGTAGCAGCCCTGGCCCCGCTGGTGGCAGCTCCTCCCC
  GTCCCAGCCAAGGCCGCCTGGCAGGACGGGAGTGGAGCACACAGGCTCACCCTAGGGACAGCCAGGGTCC
  GCGCCTCTGTGGGGAAGGTCGGGGGGCATAAACCCTGTGGGCAGCAGGCACCGTTGCACACCGTCTGCGG
  TGGGCATGGATGGCGCCGGAGCCGTGTCCAGGGGAGGGAGCAGGCCAGGTGACAGGGGCTGTCTTGGGCC
  ACTGCCCCAGTGAGTTGTGGCCAGCTAGGAGGGGAAGGACCCTGGGCGTGGGTGCCAGAGGAGGCCATGC
  TGAGAGGCCTCTCCACCGGCTCAGCCTCCCGTGGACCCCAGCCTCCCGCGCCGCCCTTGCTGCTTGTCCC
  CATCCCCTCTACAACTTGGTTCCTTCTGTGGGGGGGCCCAGCCCAGCAGGGCTCAGTGACGTGATGCTGT
  CACACGGACCCTGGGCTTCCACCCTGTCCCTGTGAGGTGCTGCTGTGGCTAGAAGGGTCCCCGTGGGCCT
  GGGGTCCTGTCTGCACCGAGGAGGGCCTGGTGTGAGTTAGGATGCAGACGACAGCCAAACAGTGCTGGGG
  AAAGCAGGGAGGACCCTGCCCTGAGCAGAGGCCATGCGCCCGCCTGTCCTGTTCCCTCTGCAGCTGTTCT
  GGGCAGGTCCATAAGAAGGTGGGTTGGGGCCTCCGACCCCCACTGCTCCGCCTGCAGGGTTGAGAAACCC
  AGCCCCATCTCACCACGGGTCCCCACACCTCTGAGGAGGTCTCAGGGCCCCCAATGGCCAGGGACCCAGA
  AGGCTCACCTGCAGCCACCCCACACATCCCTGCCACTGTGGGCATCAGAGGCCCTCTCCCTCCAGGGTGC
  ATCTCCTGGTCAGGAGGGGGGAGGCCCGGTTGCCTTAGGCCCGGCCTGCACAGGTCGTGAGGAAAAGCAC
  CTGGTTTCGGCCGGGCGCGGTGGCTCACGCTTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGAATC
  ATGCGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGCGAAACCCTCGTCCCTACTAAAAATACAAAAA
  ATTAGTTGGGCGTAGTGGTGGGTGCCTCTAATCCCAGCTACCTGGGAGGCTGAGACAGGAGAATCACTTG
  AACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATCCTGCCACTGCTCTCCAGCCTGGCGACGGAGGAATG
  CTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC000744 unedited
  GTAGCGTTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGC
  CGTGCTCTGCCTCCTGTGTTCCAGGACTTTGAACATGTCGGGGATCGCCCTCAGCAGACT
  CGCCCAGGAGAGGAAAGCATGGAGGAAAGACCACCCATTTGGTTTCGTGGCTGTCCCAAC
  AAAAAATCCCGATGGCACGATGAACCTCATGAACTGGGAGTGCGCCATTCCAGGAAAGAA
  AGGGACTCCGTGGGAAGGAGGCTTGTTTAAACTACGGATGCTTTTCAAAGATGATTATCC
  ATCTTCGCCACCAAAATGTAAATTCGAACCACCATTATTTCACCCGAATGTGTACCCTTC
  GGGGACAGTGTGCCTGTCCATCTTAGAGGAGGACAAGGACTGGAGGCCAGCCATCACAAT
  CAAACAGATCCTATTAGGAATACAGGAACTTCTAAATGAACCAAATATCCAAGACCCAGC
  TCAAGCAGAGGCCTACACGATTTACTGGTTAGTAGCAGCCCTGGCCCCGCTGGTGGCAGC
  TCCTCCCCGTCCCAGCCAAGGCCGCCTGGCAGGACGGGAGTGGAGCACACAGGCTCACCC
  TAGGGACAGCCAGGGTCCGCGCCTCTGTGGGGAAGGTCGGGGGGCATAAACCCTGTGGGC
  AGCAGGCACCGTTGCACACCGTCTGCGGTGGGCATGGATGGCGCCGGAGCCGTGTCCAGG
  GGAGGGAGCAGGCCAGGTGACAGGGGCTGTCTTGGGCCACTGCCCCAGTGAGTTGTGGCC
  AGCTAGNGAGGGAAGGACCCTGGGCGTGGGTGCCAGAGAGGCCATGCTGAGAGCCTCTCC
  ACGGCTCACCA
  
• Restriction Sites: Please inquire
• ACCN: BC000744
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC000744.1, AAH00744.1
• RefSeq Size: 1724 bp
• RefSeq ORF: 555 bp
• Locus ID: 7329
• Cytogenetics: 16p13.3
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'