GJB5 (NM_005268) Human Untagged Clone

CAT#: SC124154

GJB5 (untagged)-Human gap junction protein, beta 5, 31.1kDa (GJB5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GJB5
• Synonyms: CX31.1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124154 sequence for NM_005268 edited (data generated by NextGen Sequencing)
  ATGAACTGGAGTATCTTTGAGGGACTCCTGAGTGGGGTCAACAAGTACTCCACAGCCTTT
  GGGCGCATCTGGCTGTCTCTGGTCTTCATCTTCCGCGTGCTGGTGTACCTGGTGACGGCC
  GAGCGTGTGTGGAGTGATGACCACAAGGACTTCGACTGCAATACTCGCCAGCCCGGCTGC
  TCCAACGTCTGCTTTGATGAGTTCTTCCCTGTGTCCCATGTGCGCCTCTGGGCCCTGCAG
  CTTATCCTGGTGACATGCCCCTCACTGCTCGTGGTCATGCACGTGGCCTACCGGGAGGTT
  CAGGAGAAGAGGCACCGAGAAGCCCATGGGGAGAACAGTGGGCGCCTCTACCTGAACCCC
  GGCAAGAAGCGGGGTGGGCTCTGGTGGACATATGTCTGCAGCCTAGTGTTCAAGGCGAGC
  GTGGACATCGCCTTTCTCTATGTGTTCCACTCATTCTACCCCAAATATATCCTCCCTCCT
  GTGGTCAAGTGCCACGCAGATCCATGTCCCAATATAGTGGACTGCTTCATCTCCAAGCCC
  TCAGAGAAGAACATTTTCACCCTCTTCATGGTGGCCACAGCTGCCATCTGCATCCTGCTC
  AACCTCGTGGAGCTCATCTACCTGGTGAGCAAGAGATGCCACGAGTGCCTGGCAGCAAGG
  AAAGCTCAAGCCATGTGCACAGGTCATCACCCCCACGGTACCACCTCTTCCTGCAAACAA
  GACGACCTCCTTTCGGGTGACCTCATCTTTCTGGGCTCAGACAGTCATCCTCCTCTCTTA
  CCAGACCGCCCCCGAGACCATGTGAAGAAAACCATCTTGTGA
  
  Clone variation with respect to NM_005268.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005268 unedited
  NGGGAAACTCGGTATTTGTTAAACGACTTACTATAGGCGGCCGCGNAATTCGCACGAGGA
  TGAAATTCAAGCTGCTTGCTGAGTCCTATTGCCGGCTGCTGGGAGCCAGGAGAGCCCTGA
  GGAGTAGTCACTCAGTAGCAGCTGACGCGTGGGTCCACCATGAACTGGAGTATCTTTGAG
  GGACTCCTGAGTGGGGTCAACAAGTACTCCACAGCCTTTGGGCGCATCTGGCTGTCTCTG
  GTCTTCATCTTCCGCGTGCTGGTGTACCTGGTGACGGCCGAGCGTGTGTGGAGTGATGAC
  CACAAGGACTTCGACTGCAATACTCGCCAGCCCGGCTGCTCCAACGTCTGCTTTGATGAG
  TTCTTCCCTGTGTCCCATGTGCGCCTCTGGGCCCTGCAGCTTATCCTGGTGACATGCCCC
  TCACTGCTCGTGGTCATGCACGTGGCCTACCGGGAGGTTCAGGAGAAGAGGCACCGAGAA
  GCCCATGGGGAGAACAGTGGGCGCCTCTACCTGAACCCCGGCAAGAAGCGGGGTGGGCTC
  TGGTGGACATATGTCTGCAGCCTAGTGTTCAAGGCGAGCGTGGACATCGCCTTTCTCTAT
  GTGTTCCACTCATTCTACCCCAAATATATCCTCCCTCCTGTGGTCAAGTGCCACGCAGAT
  CCATGTCCCAATATAGTGGACTGCTTCATCTCCAAGCCCTCAGAGAAGAACATTTTCACC
  CTCTTCATGGTGGCCACAGCTGCCATCTGCATCCTGCTCAACCTCGTGGAGCTCATCTAC
  CTGGTGAGCAAGAGATGCCACGAGTGCCTGGCAGCAGGAAAGCTCAAGCATGTGCACAGG
  TCATCACCCCCACGGTACCCACCTCTTCTGCANACAAGACGACCTCTTTNCGGNTGACCT
  CATCTTTCTGGGCTCAGACAGTCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005268 unedited
  AGGGGNAGGGNNTNNNNTTGCCTGGATGGCACTTCCAGGCCAGGAGAGGCACTGGGGAGG
  GGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCT
  AGAGTCGAGTTTTTTTTTTTTTTTTTTCCAGCAAATGAGTTGACTTTTATTAAGTTCTGA
  CAGTGGGCCACACACGATGAAGGTGCCCGCATGTGTGTTCTTCTATGAACGCCCTCCTCC
  CTCCAACCCTGGGCATTGGCCCTCACTATTTCCATTCTAGAAAAGGAAACCGAGCTGCAG
  AGCAGAGGGGGAACTGGCCCAGTGCCGTCGAGCTGGGGCAGGTGGCTGGAGTTGAGGACT
  AGGAACTGGCTCCCAGAGCGTCTGAATCCTCTCTCTTGCCTGCCCCTACCTCATGGCTTA
  GCTCCCCCACTCTCAGGTTGCACCTATGAGAGATGCTAGAGCCTCCCCATCCAGGCCCAA
  CCTGCCAGACCAGTCCAGGCAGCCCCTCACAAGATGGTTTTCTTCACATGGTCTCGGGGG
  CGGTCTGGTAAGAGAGGAGGATGACTGTCTGAGCCCAGAAAGATGAGGTCACCCGAAAGG
  AGGTCGTCTTGTTTGCAGGAAGAGGTGGTACCGTGGGGGTGATGACCTGTGCACATGGCT
  TGAGCTTTCCTTGCTGCCAGGCACTCGTGGCATCTCTTGCTCACCAGGTAGATGAGCTCC
  ACGAGGTTGAGCAGGATGCANATGGCAGCTGTGGCCACCATGAAGAGGGTGAAAATGTTC
  TTCTCTGAGN
  
• Restriction Sites: Please inquire
• ACCN: NM_005268
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005268.2, NP_005259.1
• RefSeq Size: 1299 bp
• RefSeq ORF: 822 bp
• Locus ID: 2709
• Cytogenetics: 1p34.3
• Protein Families: Ion Channels: Other, Transmembrane
• Gene Summary: 'This gene encodes a member of the beta-type (group I) connexin family. The encoded protein is a gap junction protein involved in intercellular communication related to epidermal differentiation and environmental sensing. This gene has been linked to non-small cell lung cancer. [provided by RefSeq, Nov 2012]'