CACNA2D1 (NM_000722) Human Untagged Clone

CAT#: SC124161

CACNA2D1 (untagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 1 (CACNA2D1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNA2D1
• Synonyms: CACNA2; CACNL2A; CCHL2A; LINC01112; lncRNA-N3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000722 edited
  ATGGCTGCTGGCTGCCTGCTGGCCTTGACTCTGACACTTTTCCAATCTTTGCTCATCGGC
  CCCTCGTCGGAGGAGCCGTTCCCTTCGGCCGTCACTATCAAATCATGGGTGGATAAGATG
  CAAGAAGACCTTGTCACACTGGCAAAAACAGCAAGTGGAGTCAATCAGCTTGTTGATATT
  TATGAGAAATATCAAGATTTGTATACTGTGGAACCAAATAATGCACGCCAGCTGGTAGAA
  ATTGCAGCCAGGGATATTGAGAAACTTCTGAGCAACAGATCTAAAGCCCTGGTGCGCCTG
  GCATTGGAAGCGGAGAAAGTTCAAGCAGCTCACCAGTGGAGAGAAGATTTTGCAAGCAAT
  GAAGTTGTCTACTACAATGCAAAGGATGATCTCGATCCTGAGAAAAATGACAGTGAGCCA
  GGCAGCCAGAGGATAAAACCTGTTTTCATTGAAGATGCTAATTTTGGACGACAAATATCT
  TATCAGCACGCAGCAGTCCATATTCCTACTGACATCTATGAGGGCTCAACAATTGTGTTA
  AATGAACTCAACTGGACAAGTGCCTTAGATGAAGTTTTCAAAAAGAATCGCGAGGAAGAC
  CCTTCATTATTGTGGCAGGTTTTTGGCAGTGCCACTGGCCTAGCTCGATATTATCCAGCT
  TCACCATGGGTTGATAATAGTAGAACTCCAAATAAGATTGACCTTTATGATGTACGCAGA
  AGACCATGGTACATCCAAGGAGCTGCATCTCCTAAAGACATGCTTATTCTGGTGGATGTG
  AGTGGAAGTGTTAGTGGATTGACACTTAAACTGATCCGAACATCTGTCTCCGAAATGTTA
  GAAACCCTCTCAGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAATGCTCAGGAT
  GTAAGCTGTTTTCAGCACCTTGTCCAAGCAAATGTAAGAAATAAAAAAGTGTTGAAAGAC
  GCGGTGAATAATATCACAGCCAAAGGAATTACAGATTATAAGAAGGGCTTTAGTTTTGCT
  TTTGAACAGCTGCTTAATTATAATGTTTCCAGAGCAAACTGCAATAAGATTATTATGCTA
  TTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTAACAAATACAATAAAGATAAA
  AAAGTACGTGTATTCACGTTTTCAGTTGGTCAACACAATTATGACAGAGGACCTATTCAG
  TGGATGGCCTGTGAAAACAAAGGTTATTATTATGAAATTCCTTCCATTGGTGCAATAAGA
  ATCAATACTCAGGAATATTTGGATGTTTTGGGAAGACCAATGGTTTTAGCAGGAGACAAA
  GCTAAGCAAGTCCAATGGACAAATGTGTACCTGGATGCATTGGAACTGGGACTTGTCATT
  ACTGGAACTCTTCCGGTCTTCAACATAACCGGCCAATTTGAAAATAAGACAAACTTAAAG
  AACCAGCTGATTCTTGGTGTGATGGGAGTAGATGTGTCTTTGGAAGATATTAAAAGACTG
  ACACCACGTTTTACACTGTGCCCCAATGGGTATTACTTTGCAATCGATCCTAATGGTTAT
  GTTTTATTACATCCAAATCTTCAGCCAAAGAACCCCAAATCTCAGGAGCCAGTAACATTG
  GATTTCCTTGATGCAGAGTTAGAGAATGATATTAAAGTGGAGATTCGAAATAAGATGATT
  GATGGGGAAAGTGGAGAAAAAACATTCAGAACTCTGGTTAAATCTCAAGATGAGAGATAT
  ATTGACAAAGGAAACAGGACATACACATGGACACCTGTCAATGGCACAGATTACAGTTTG
  GCCTTGGTATTACCAACCTACAGTTTTTACTATATAAAAGCCAAACTAGAAGAGACAATA
  ACTCAGGCCAGATCAAAAAAGGGCAAAATGAAGGATTCGGAAACCCTGAAGCCAGATAAT
  TTTGAAGAATCTGGCTATACATTCATAGCACCAAGAGATTACTGCAATGACCTGAAAATA
  TCGGATAATAACACTGAATTTCTTTTAAATTTCAACGAGTTTATTGATAGAAAAACTCCA
  AACAACCCATCATGTAACGCGGATTTGATTAATAGAGTCTTGCTTGATGCAGGCTTTACA
  AATGAACTTGTCCAAAATTACTGGAGTAAGCAGAAAAATATCAAGGGAGTGAAAGCACGA
  TTTGTTGTGACTGATGGTGGGATTACCAGAGTTTATCCCAAAGAGGCTGGAGAAAATTGG
  CAAGAAAACCCAGAGACATATGAGGACAGCTTCTATAAAAGGAGCCTAGATAATGATAAC
  TATGTTTTCACTGCTCCCTACTTTAACAAAAGTGGACCTGGTGCCTATGAATCGGGCATT
  ATGGTAAGCAAAGCTGTAGAAATATATATTCAAGGGAAACTTCTTAAACCTGCAGTTGTT
  GGAATTAAAATTGATGTAAATTCCTGGATAGAGAATTTCACCAAAACCTCAATCAGAGAT
  CCGTGTGCTGGTCCAGTTTGTGACTGCAAAAGAAACAGTGACGTAATGGATTGTGTGATT
  CTGGATGATGGTGGGTTTCTTCTGATGGCAAATCATGATGATTATACTAATCAGATTGGA
  AGATTTTTTGGAGAGATTGATCCCAGCTTGATGAGACACCTGGTTAATATATCAGTTTAT
  GCTTTTAACAAATCTTATGATTATCAGTCAGTATGTGAGCCCGGTGCTGCACCAAAACAA
  GGAGCAGGACATCGCTCAGCATATGTGCCATCAGTAGCAGACATATTACAAATTGGCTGG
  TGGGCCACTGCTGCTGCCTGGTCTATTCTACAGCAGTTTCTCTTGAGTTTGACCTTTCCA
  CGACTCCTTGAGGCAGTTGAGATGGAGGATGATGACTTCACGGCCTCCCTGTCCAAGCAG
  AGCTGCATTACTGAACAAACCCAGTATTTCTTCGATAACGACAGTAAATCATTCAGTGGT
  GTATTAGACTGTGGAAACTGTTCCAGAATCTTTCATGGAGAAAAGCTTATGAACACCAAC
  TTAATATTCATAATGGTTGAGAGCAAAGGGACATGTCCATGTGACACACGACTGCTCATA
  CAAGCGGAGCAGACTTCTGACGGTCCAAATCCTTGTGACATGGTTAAGCAACCCAGATAC
  CGAAAAGGGCCTGATGTCTGCTTTGATAACAATGTCTTGGAGGATTATACTGACTGTGGT
  GGTGTTTCTGGATTAAATCCCTCCCTGTGGTATATCATTGGAATCCAGTTTCTACTACTT
  TGGCTGGTATCTGGCAGCACACACCGCCTGTTATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000722 unedited
  TTTTGAATACGACTGACTATAGGGCGGCCGCGAATTCGGCACGAGGCGAGCGCCGGCGGG
  CTCGCCGAGGTCTGTTTCCAAAGTCGCCCTTGATGGCGGCGGAGGCAAGGCGGCCNNNNN
  GCGGAGCAGCCGACGCACGCTAGTGGGTCCGCCCGCCACCGCCCCTTCCTCGGCGTCCGC
  TCCCGCCCTTGCCGTCCCCCGCGCGGCTCCGCGCCTCGGGCCCCGGGCGCAGCCAGCCCT
  CCAGACGCCCGCGGTCCCGGCGGCGTGTGCTGCTCTTCCTCCGCCCGCGGTTTCCAGCGC
  CGCTCCTTCCCCCGCTTGGGCAGGGAGGGGGCATTGATCTTCGATCGCGAAGATGGCTGC
  TGGCTGCCTGCTGGCCTTGACTCTGACACTTTTCCAATCTTTGCTCATCGGCCCCTCGTC
  GGAGGAGCCGTTCCCTTCGGCCGTCACTATCAAATCATGGGTGGATAAGATGCAAGAAGA
  CCTTGTCACACTGGCAAAAACAGCAAGTGGAGTCAATCAGCTTGTTGATATTTATGAGAA
  ATATCAAGATTTGTATACTGTGGAACCAAATAATGCACGCCAGCTGGTAGAAATTGCAGC
  CAGGGATATTGAGAAACTTCTGAGGCACAGATCTAAAGCCCTGGTGCGCCTGGCATTGGN
  AAGCGAGAAAGTTCAGCAGCTCACCAGTGGAGAAGAGATTTTTGCAAGCATGAAGTTGTC
  TACTACAATGCANAAGATGATCTCGATCCCTGAGAAAATGACGTGAGCCAGGCAGCCGA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_000722 unedited
  NAAGAGAGCGGCCGATGGTTAAGCAACCCAAAACCGAAAAGNGCCTGAGTCTGCTTTGAT
  AACAATGTCTTGGAGGATTATACTGACTGTGGTGGTGTTTCTGGATTAAATCCCTCCCTG
  TGGTATATCATTGGAATCCAGTTTCTACTACTTTGGCTGGTATCTGGCAGCACACACCGC
  CTGTTATGACCTTCTAAAAACCAAATCTGCATAGTTAAACTCCAGACCCTGCCAAAACAT
  GAGCCCTGCCCTCAATTACAGTAACGTAGGGTCAGCTATAAAATCAGACAAACATTAGCT
  GGGCCTGTTCCATGGCATAACACTAAGGCGCAGACTCCTAAGGCACCCACTGGCTGCATG
  TCAGGGTGTCAGATCCTTAAACGTGTGTGAATGCTGCATCATCTATGTGTAACATCAAAG
  CAAAATCCTATACGTGTCCTCTATTGGAAAATTTGGGAGTTTGTTGTTGCATTGTTGGTG
  ATTACATGTGAAAGGGTTCCCCATACAATTGTTAATGAACCATAAGAAATGTCTTGATAT
  TGACCTGGAATTTTGACTTGCTGCAATTTTACTAAGAAAATCTCTAAGGGGAAAGAAATT
  ATTTTTGCCTTCACTTTTTCTTCTGTGANAAGTTAATTCCTGCTTTAAGTAGCAATTATT
  GAAATATATAGAGAAAGAAGAGATTAACATTGGTCTAAATTGTTGAAATATANATAATGG
  CTAATTTTCTATGAAAAATTTGCCATGAATAANATGCCTTATGAANAACGGCTTCTTTGC
  CAAAAATAAACACATTTGATGACCAATTCATTTTGATGNGTAATGAATTGATGGTTTTGA
  AATAGTAACTAANAAATTATTAAACTAAAGGTGCTTGAGGAAGAAATTTAATATGT
  
• Restriction Sites: Please inquire
• ACCN: NM_000722
• Insert Size: 6400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000722.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000722.2, NP_000713.2
• RefSeq Size: 3822 bp
• RefSeq ORF: 3276 bp
• Locus ID: 781
• Cytogenetics: 7q21.11
• Domains: VWA, Cache
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• Gene Summary: 'The preproprotein encoded by this gene is cleaved into multiple chains that comprise the alpha-2 and delta subunits of the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. Mutations in this gene can cause cardiac deficiencies, including Brugada syndrome and short QT syndrome. Alternate splicing results in multiple transcript variants, some of which may lack the delta subunit portion. [provided by RefSeq, Nov 2014]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.