IDH3G (NM_174869) Human Untagged Clone

CAT#: SC124266

IDH3G (untagged)-Human isocitrate dehydrogenase 3 (NAD+) gamma (IDH3G), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IDH3G
• Synonyms: H-IDHG
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_174869, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGCTGAAGGTAGCGACCGTCGCCGGCAGCGCCGCGAAGGCGGTGCTCGGGCCAGCCCTTCTCTGCC
  GTCCCTGGGAGGTTCTAGGCGCCCACGAGGTCCCCTCGAGGAACATCTTTTCAGAACAAACAATTCCTCC
  GTCCGCTAAGTATGGCGGGCGGCACACGGTGACCATGATCCCAGGGGATGGCATCGGGCCAGAGCTCATG
  CTGCATGTCAAGTCCGTCTTCAGGCACGCATGTGTACCAGTGGACTTTGAAGAGGTGCACGTGAGTTCCA
  ATGCTGATGAAGAGGACATTCGCAATGCCATCATGGCCATCCGCCGGAACCGCGTGGCCCTGAAGGGCAA
  CATCGAAACCAACCATAACCTGCCACCGTCGCACAAATCTCGAAACAACATCCTTCGCACCAGCCTGGAC
  CTCTATGCCAACGTCATCCACTGTAAGAGCCTTCCAGGCGTGGTGACCCGGCACAAGGACATAGACATCC
  TCATTGTCCGGGAGAACACAGAGGGCGAGTACAGCAGCCTGGAGCATGAGAGTGTGGCGGGAGTGGTGGA
  GAGCCTGAAGATCATCACCAAGGCCAAGTCCCTGCGCATTGCCGAGTATGCCTTCAAGCTGGCGCAGGAG
  AGCGGGCGCAAGAAAGTGACGGCCGTGCACAAGGCCAACATCATGAAACTGGGCGATGGGCTTTTCCTCC
  AGTGCTGCAGGGAGGTGGCAGCCCGCTACCCTCAGATCACCTTCGAGAACATGATTGTGGATAACACCAC
  CATGCAGCTGGTGTCCCGGCCCCAGCAGTTTGATGTCATGGTGATGCCCAATCTCTATGGCAACATCGTC
  AACAATGTCTGCGCGGGACTGGTCGGGGGCCCAGGCCTTGTGGCTGGGGCCAACTATGGCCATGTGTACG
  CGGTGTTTGAAACAGCTACGAGGAACACCGGCAAGAGTATCGCCAATAAGAACATCGCCAACCCCACGGC
  CACCCTGCTGGCCAGCTGCATGATGCTGGACCACCTCAAGCTGCACTCCTATGCCACCTCCATCCGTAAG
  GCTGTCCTGGCATCCATGGACAATGAGAATGTGAGGTTCCCCTCGCACCCTACCCTGCTGCCCCGCCCAG
  TCTCCCCCTGCAGCCTCCTGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_174869 unedited
  GGGGGGNNNNNNNNNNNNNNNCNNCCNNNCNNCNNNCGGTTCAGAATTGTATACGATCAT
  ATAGGCGGCCGCGNAATTCGCACCAGGTCGGGCCGTGCGGAGGCTCTCACTTTCCGTCTG
  GCGCTGAAGGTAGCGACCGTCGCCGGCAGCGCCGCGAAGGCGGTGCTCGGGCCAGCCCTT
  CTCTGCCGTCCCTGGGAGGTTCTAGGCGCCCACGAGGTCCCCTCGAGGAACATCTTTTCA
  GAACAAACAATTCCTCCGTCCGCTAAGTATGGCGGGCGGCACACGGTGACCATGATCCCA
  GGGGATGGCATCGGGCCAGAGCTCATGCTGCATGTCAAGTCCGTCTTCAGGCACGCATGT
  GTACCAGTGGACTTTGAAGAGGTGCACGTGAGTTCCAATGCTGATGAAGAGGACATTCGC
  AATGCCATCATGGCCATCCGCCGGAACCGCGTGGCCCTGAAGGGCAACATCGAAACCAAC
  CATAACCTGCCACCGTCGCACAAATCTCGAAACAACATCCTTCGCACCAGCCTGGACCTC
  TATGCCAACGTCATCCACTGTAAGAGCCTTCCAGGCGTGGTGACCCGGCACAAGGACATA
  GACATCCTCATTGTCCGGGAGAACACAGAGGGCGAGTACAGCAGCCTGGAGCATGAAAGT
  GNTGCGGGAGTGGTGGAAGCCTGAAGACATCACCAAGCCAAGTCCCTGCGCATTGCCGAG
  TATGCCTTNCAGNNCTGCGCAGAGAAGCGGCGCAAGAAAGTGACNGCCGTGCACAGGNCC
  ACATCATGAAACTGGGCGATGGGCTTTCCTCCAGTGCTGCAGGNNAGTGGCANCCNGCTA
  CCCNAGATCACCTTCAGACATGATTGTGATACAACACATGCAGCTGGTGTCCGGCCCCAG
  CAGTTGAGTCATGGGATGCCCATCTCTATGCACATCGTCACATGTCTGCGGAACTGGTCG
  GGGCCCAGGCCTGGGGCTGGGGCAACTTGGCATGTGTACCCGTGTTTGAACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_174869 unedited
  NCGTATACTATGNACGCGGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTTTTTAG
  GGAGAAGGTGCTTTATTCTGGGATCTGCGTACCAAGCTGGCTGGGGTGCTGGAGTGGGAA
  GGGGAATCCAAGGAGCAAACCAAGAAGGTCCTAGGGCCAGCCTAGGCCTCCACGGCCCGG
  CCGTTGATGACGCGGATGTGGCGGATGACGTCCTGGATGGCTTCAGATGTTGTGCCCTGG
  CCCCCGATGTCCGGAGTGTGCATCTGTAGGACACAGGCAGGCTCGGCACACACCAGGCCC
  TGCCACCCCCCGCTGTCTCCTGTGACGTGCAGGACTGGGATTGCCACAGGAGGGAAGTGG
  CACCAGCTAAGGGCCAGAAGATGGGGCCAGATCCAGTACCATCCTCCCCTGGGGGCTGTG
  GTCAGTGCATGAGGCGGGCTACAGGAGGCTGCAGGGGGAGACTGGGCGGGGCAGCAGGGT
  AGGGTGCGAGGGGAACCTCACATTCTCATTGTCCATGGATGCCAGGACAGCCTTACGGAT
  GGAGGTGGCATATGAGTGCAGCTTGAGGTGGTCCAGCATCATGCAGCTGGCCAGCAGGGT
  GGCCGTGGGGTTGGCGATGTTCTTATTGGCGATACTCTTGCCGGTGTTCCTCGTAGCTGT
  TTCAAACACCGCGTACACATGGCCATAGTTGGCCCCAGCCACAAGGCCTGGGCCCCCGAC
  CAGTCCCGCGCAGACATTGTTGACGATGTTGCCATAGAGATTGGGCATCACCATGACATC
  AAACTGCTGGGGCCGGGACACCANCTGCATGGTGGTGGTATCCACAATCATGTTCTCGAA
  GGTGATCTTGAGGTANCGGGCTGCCACCTTCCTGCAGCACTGGGAGAAAAGCCATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_174869
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174869.1, NP_777358.1
• RefSeq Size: 1723 bp
• RefSeq ORF: 1143 bp
• Locus ID: 3421
• Cytogenetics: Xq28
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways
• Gene Summary: 'Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 3' UTR and coding region resulting in a frameshift, compared to variant 1. Variant 2 encodes a shorter isoform (b) with a distinct C-terminus, compared to isoform a.