MAG (NM_080600) Human Untagged Clone

CAT#: SC124271

MAG (untagged)-Human myelin associated glycoprotein (MAG), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAG
• Synonyms: GMA; S-MAG; SIGLEC-4A; SIGLEC4A; SPG75
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_080600, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATATTCCTCACGGCACTGCCTCTGTTCTGGATTATGATTTCAGCCTCCCGAGGGGGTCACTGGGGTG
  CCTGGATGCCCTCGTCCATCTCGGCCTTCGAAGGCACGTGCGTCTCCATCCCCTGCCGCTTTGACTTCCC
  GGATGAGCTGCGGCCCGCTGTGGTGCATGGTGTCTGGTACTTCAATAGCCCCTACCCCAAGAACTACCCC
  CCGGTGGTCTTCAAGTCGCGCACCCAAGTAGTCCACGAGAGCTTCCAGGGCCGCAGCCGCCTCCTGGGGG
  ACCTGGGCCTGCGAAACTGCACCCTCCTGCTCAGCAACGTCAGCCCCGAGCTGGGCGGGAAGTACTACTT
  CCGTGGGGACCTGGGCGGCTACAACCAGTACACCTTCTCAGAGCACAGCGTCCTGGATATCGTCAACACC
  CCCAACATCGTGGTGCCCCCAGAGGTGGTGGCAGGCACGGAGGTGGAGGTCAGCTGCATGGTGCCGGACA
  ACTGCCCAGAGCTGCGCCCTGAGCTGAGCTGGCTGGGCCACGAGGGGCTGGGGGAGCCCGCTGTGCTGGG
  CCGGCTGCGGGAGGACGAGGGCACCTGGGTGCAGGTGTCACTGCTGCACTTCGTGCCCACGAGGGAGGCC
  AACGGCCACAGGCTGGGCTGCCAGGCCTCCTTCCCCAACACCACCCTGCAGTTCGAGGGCTACGCCAGCA
  TGGACGTCAAGTACCCCCCGGTGATTGTGGAGATGAACTCCTCGGTGGAGGCCATCGAGGGCTCCCACGT
  GAGCCTGCTCTGTGGGGCTGACAGCAACCCCCCGCCGCTGCTGACCTGGATGCGGGACGGGACAGTCCTC
  CGGGAGGCGGTGGCCGAGAGCCTGCTCCTGGAGCTGGAGGAGGTGACCCCCGCCGAAGACGGCGTCTATG
  CCTGCCTGGCCGAGAATGCCTATGGCCAGGACAACCGCACCGTGGGGCTCAGTGTCATGTATGCACCCTG
  GAAGCCAACAGTGAACGGGACAATGGTGGCCGTAGAGGGGGAGACGGTCTCTATCTTGTGCTCCACACAG
  AGCAACCCGGACCCTATTCTCACCATCTTCAAGGAGAAGCAGATCCTGTCCACGGTCATCTACGAGAGCG
  AGCTGCAGCTGGAGCTGCCGGCCGTGTCACCCGAGGATGATGGAGAGTACTGGTGTGTGGCTGAGAACCA
  GTATGGCCAGAGGGCCACCGCCTTCAACCTGTCTGTGGAGTTCGCCCCTGTGCTCCTCCTGGAGTCCCAC
  TGCGCGGCAGCCCGAGACACGGTGCAGTGCCTGTGCGTGGTGAAGTCCAACCCGGAGCCGTCCGTGGCCT
  TTGAGCTGCCATCGCGCAATGTGACCGTGAACGAGAGCGAGCGGGAGTTCGTGTACTCGGAGCGCAGCGG
  CCTCGTGCTCACCAGCATCCTCACGCTGCGGGGGCAGGCCCAGGCCCCGCCCCGCGTCATCTGCACCGCG
  AGGAACCTCTATGGCGCCAAGAGCCTGGAGCTGCCCTTCCAGGGAGCCCATCGACTGATGTGGGCCAAGA
  TCGGGCCTGTGGGCGCCGTGGTCGCCTTTGCCATCCTGATTGCCATCGTCTGCTACATTACCCAGACACG
  CAGGAAAAAGAACGTGACAGAGAGCCCCAGCTTCTCGGCAGGGGACAACCCTCCCGTCCTGTTCAGCAGC
  GACTTCCGCATCTCTGGGGCACCAGAGAAGTACGAGTCCAAAGAGGTTTCTACCCTGGAATCTCACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080600 unedited
  CAAAATTTGGTATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGGGGGNAGCAGAG
  GTGCAGAAGCAACTGAGTCCAAGTTGTCTGGCGGCTTCAGGTGGACCCAGAAGACGTCCC
  CAACTCAGGGAGATTCAGCGATCACTCACTCGCTGTACAGAATGATATTCCTCACGGCAC
  TGCCTCTGTTCTGGATTATGATTTCAGCCTCCCGAGGGGGTCACTGGGGTGCCTGGATGC
  CCTCGTCCATCTCGGCCTTCGAAGGCACGTGCGTCTCCATCCCCTGCCGCTTTGACTTCC
  CGGATGAGCTGCGGCCCGCTGTGGTGCATGGTGTCTGGTACTTCAATAGCCCCTACCCCA
  AGAACTACCCCCCGGTGGTCTTCAAGTCGCGCACCCAAGTAGTCCACGAGAGCTTCCAGG
  GCCGCAGCCGCCTCCTGGGGGACCTGGGCCTGCGAAACTGCACCCTCCTGCTCAGCAACG
  TCAGCCCCGAGCTGGGCGGGAAGTACTACTTCCGTGGGGACCTGGGCGGCTACAACCAGT
  ACACCTTCTCAGAGCACAGCGTCCTGGATATCGTCAACACCCAAATCCAGGGGGGGTGCC
  CCCAGAGGTGGTGGCAGCACGGAGGTGGGAAGTCAGCTGCATGGTGCCGGACAACTGCCC
  AGAGCTGCGCCCTGAGCTGAGCTGGGCTGGCCACGAGGGGGCTGGGGGGAGCCCCGCTGT
  TGCTNGGGCCCGGCTGCNGGAGGGACNAGGGCACCTGGTGGCCCAAGGTGTCACTGCTGC
  ACTTCGTGCCCGGNAGGGGAGGCCAACGGNCACAGGCTTGGGCTGCCAGGCCTCCTTTCC
  ACACCCCTGGANTNNNNGAGGGCTCGCGCCTAGGGACGTCAGACCCCCGGGGGGATGGGG
  GAAATGACTCCTGGGGGAGCCCCTCCAAGGCTCCCGTGAGCTGTTCTGGGGGGGCTGAAG
  CACCCCCCGCCTGTGACTGATGTCGGAAGGGCATCTCCGGGGGGGCGGGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_080600 unedited
  GGGCCATTTATTTCATGTCCCTTTTGTACAGCCCCAGNGCCAGTGTGGGGGCAGGAGACC
  AGGCAGGAAGTAGCAATAAATAACACGGACAGACAGAGCTCCCCTCCAGACAGATGAGGG
  TAAAGGAGGTTAGAGAGGAGCCGTAATGAGGGCGTGGGGGTGCTGGCAGGGAGGGGCAGC
  TGGGAAGGAGGGGGAGGTCAGGCCCCCAGGACCTCACTGTCACCTGCCTCACTCCCCTCC
  TGCCCCAGCCCCCGATACTTTTGGGAGGAAGGGAGCCCACAGCCAGTGGGGGCCAGCGAG
  GGTCCTGAGGGGGGGTCANCCACGCAGGCTGCCCCCAGCTCCTTCACTTGACCCGGATTT
  CAGCATACTCAGCTAGCTCCTCCGTCAGCGTGTAGCTGTCCTTGGTGGGCCGTTTCCCCA
  GGTCCGAGTGAGAATAGCTCAAGTCCAGCTCTGGGGGCTCACCCCGAAGGCCCAGCAGCC
  TCCTCTCAGATCCCAGGCGCCTCTCGCTCTCGTACTTCTCTGGTGCCCCAGAGATGCGGA
  AGTCGCTGCTGAACAGGACGGGAGGGTTGTCCCCTGCCGAGAAGCTGGGGCTCTCTGTCA
  CGTTCTTTTTCCTGCGTGTCTGGGTAATGTAGCAGACGATGGCAATCAGGATGGCAAAGG
  CGACCACGGCGCCCACAGGCCCGATCTTGGCCCACATCAGTCGATGGGCTCCCTGGAGGG
  CAGCTCCCAGCTCTTGCGCCATAGAGGTTCCTCGCGGTGCAAATGACGCGGGGCGGGGGC
  CTGGGCCTGCCCCCGCAACGTGAGGATGCTGGTGAGCACCAAGCCGCTGCGCTCCCAATA
  CACGAACTCCCGCTCGCTCTTCGTCACGGTCACATTGCGCGATGGCAGCTTAAAAGCCAC
  GGACGGCTCCCGGTTGGACTTTACCACCCACAGCACTGGACCGGGTTTTGGGCTGTCCGC
  CAGTGGACTTCAGAAGAACCAAAGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_080600
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080600.1, NP_542167.1
• RefSeq Size: 2503 bp
• RefSeq ORF: 1749 bp
• Locus ID: 4099
• Cytogenetics: 19q13.12
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: 'The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (2) is a full-length transcript with the stop codon contained in exon 11. It encodes an isoform (b) that is 44 aa shorter and contains a different carboxy terminus when compared to isoform a.