ECM1 (NM_022664) Human Untagged Clone

CAT#: SC124297

ECM1 (untagged)-Human extracellular matrix protein 1 (ECM1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ECM1
• Synonyms: URBWD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124297 sequence for NM_022664 edited (data generated by NextGen Sequencing)
  ATGGGGACCACAGCCAGAGCAGCCTTGGTCTTGACCTATTTGGCTGTTGCTTCTGCTGCC
  TCTGAGGGAGGCTTCACGGCTACAGGACAGAGGCAGCTGAGGCCAGAGCACTTTCAAGAA
  GTTGGCTACGCAGCTCCCCCCTCCCCACCCCTATCCCGAAGCCTCCCCATGGATCACCCT
  GACTCCTCTCAGCATGGCCCTCCCTTTGAGGGACAGAGTCAAGTGCAGCCCCCTCCCTCT
  CAGGAGGCCACCCCTCTCCAACAGGAAAAGCTGCTACCTGCCCAACTCCCTGCTGAAAAG
  GAAGTGGGTCCCCCTCTCCCTCAGGAAGCTGTCCCCCTCCAAAAAGAGCTGCCCTCTCTC
  CAGCACCCCAATGAACAGAAGGAAGGAATGCCAGCTCCATTTGGGGACCAGAGCCATCCA
  GAACCTGAGTCCTGGAATGCAGCCCAGCACTGCCAACAGGACCGGTCCCAAGGGGGCTGG
  GGCCACCGGCTGGATGGCTTCCCCCCTGGGCGGCCTTCTCCAGACAATCTGAACCAAATC
  TGCCTTCCTAACCGTCAGCATGTGGTATATGGTCCCTGGAACCTACCACAGTCCAGCTAC
  TCCCACCTCACTCGCCAGGGTGAGACCCTCAATTTCCTGGAGATTGGATATTCCCGCTGC
  TGCCACTGCCGCAGCCACACAAACCGCCTAGAGTGTGCCAAACTTGTGTGGGAGGATACC
  CTTGACAAATACTGTGACCGGGAGTATGCTGTGAAGACCCACCACCACTTGTGTTGCCGC
  CACCCTCCCAGCCCTACTCGGGATGAGTGCTTTGCCCGTCGGGCTCCTTACCCCAACTAT
  GACCGGGACATCTTGACCATTGACATCAGTCGAGTCACCCCCAACCTCATGGGCCACCTC
  TGTGGAAACCAAAGAGTTCTCACCAAGCATAAACATATTCCTGGGCTGATCCACAACATG
  ACTGCCCGCTGCTGTGACCTGCCATTTCCAGAACAGGCCTGCTGTGCAGAGGAGGAGAAA
  TTAACCTTCATCAATGATCTGTGTGGTCCCCGACGTAACATCTGGCGAGACCCTGCCCTC
  TGCTGTTACCTGAGTCCTGGGGATGAACAGGTCAACTGCTTCAACATCAATTATCTGAGG
  AACGTGGCTCTAGTGTCTGGAGACACTGAGAACGCCAAGGGCCAGGGGGAGCAGGGCTCA
  ACTGGAGGAACAAATATCAGCTCCACCTCTGAGCCCAAGGAAGAATGA
  
  Clone variation with respect to NM_022664.2
  389 c=>t;868 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022664 unedited
  AAAAGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGCTTCGTG
  GCCGGCCCTTCACATCCAGACTTGCCTGAGAGGACCCACCTCTGAGTGTCCAGTGGTCAG
  TTGCCCCAGGATGGGGACCACAGCCAGAGCAGCCTTGGTCTTGACCTATTTGGCTGTTGC
  TTCTGCTGCCTCTGAGGGAGGCTTCACGGCTACAGGACAGAGGCAGCTGAGGCCAGAGCA
  CTTTCAAGAAGTTGGCTACGCAGCTCCCCCCTCCCCACCCCTATCCCGAAGCCTCCCCAT
  GGATCACCCTGACTCCTCTCAGCATGGCCCTCCCTTTGAGGGACAGAGTCAAGTGCAGCC
  CCCTCCCTCTCAGGAGGCCACCCCTCTCCAACAGGAAAAGCTGCTACCTGCCCAACTCCC
  TGCTGAAAAGGAAGTGGGTCCCCCTCTCCCTCAGGAAGCTGTCCCCCTCCAAAAAGAGCT
  GCCCTCTCTCCAGCACCCCAATGAACAGAAGGAAGGAATGCCAGCTCCATTTGGGGACCA
  GAGCCATCCAGAACCTGAGTCCTGGAATGCAGCCCAGCACTGCCAACAGGACCGGTCCCA
  AGGGGGCTGGGGCCACCGGCTGGATGGCTTCCCCCCTGAGCGGCCTTCTCCAGACAATCT
  GAACCAAATCTGCCTTCCTAACCGTCAGCATGTGGTATATGGTCCCTGGAACCTACCACA
  GTCCAGCTACTCCCACCTCACTCGCCAGGGTGAGACCCTCATTTCCTGGAGATTGGATAT
  TCCCGCTGCTGCCACTGCCGCAGCCACACAAACCGCCTAGAGTGTGCCAAACTTGTGTGG
  GGAGAAGCAATGAGCCCGATCTGTGGAGCCCGAGTTCTCGGTCAGACCCGACCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022664
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022664.1, NP_073155.1
• RefSeq Size: 1462 bp
• RefSeq ORF: 1248 bp
• Locus ID: 1893
• Cytogenetics: 1q21.2
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1, resulting in a shorter isoform (2) missing an internal protein segment compared to isoform 1.