NUR77 (NR4A1) (NM_173157) Human Untagged Clone

CAT#: SC124495

NR4A1 (untagged)-Human nuclear receptor subfamily 4, group A, member 1 (NR4A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NR4A1
• Synonyms: GFRP1; HMR; N10; NAK-1; NGFIB; NP10; NUR77; TR3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_173157, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCTGTATCCAAGCCCAATATGGGACACCAGCACCGAGTCCGGGACCCCGTGACCACCTGGCAAGCG
  ACCCCCTGACCCCTGAGTTCATCAAGCCCACCATGGACCTGGCCAGCCCCGAGGCAGCCCCCGCTGCCCC
  CACTGCCCTGCCCAGCTTCAGCACCTTCATGGACGGCTACACAGGAGAGTTTGACACCTTCCTCTACCAG
  CTGCCAGGAACAGTCCAGCCATGCTCCTCAGCCTCCTCCTCGGCCTCCTCCACATCCTCGTCCTCAGCCA
  CCTCCCCTGCCTCTGCCTCCTTCAAGTTCGAGGACTTCCAGGTGTACGGCTGCTACCCCGGCCCCCTGAG
  CGGCCCAGTGGATGAGGCCCTGTCCTCCAGTGGCTCTGACTACTATGGCAGCCCCTGCTCGGCCCCGTCG
  CCCTCCACGCCCAGCTTCCAGCCGCCCCAGCTCTCTCCCTGGGATGGCTCCTTCGGCCACTTCTCGCCCA
  GCCAGACTTACGAAGGCCTGCGGGCATGGACAGAGCAGCTGCCCAAAGCCTCTGGGCCCCCACAGCCTCC
  AGCCTTCTTTTCCTTCAGTCCTCCCACCGGCCCCAGCCCCAGCCTGGCCCAGAGCCCCCTGAAGTTGTTC
  CCCTCACAGGCCACCCACCAGCTGGGGGAGGGAGAGAGCTATTCCATGCCTACGGCCTTCCCAGGTTTGG
  CACCCACTTCTCCACACCTTGAGGGCTCGGGGATACTGGATACACCCGTGACCTCAACCAAGGCCCGGAG
  CGGGGCCCCAGGTGGAAGTGAAGGCCGCTGTGCTGTGTGTGGGGACAACGCTTCATGCCAGCATTATGGT
  GTCCGCACATGTGAGGGCTGCAAGGGCTTCTTCAAGCGCACAGTGCAGAAAAACGCCAAGTACATCTGCC
  TGGCTAACAAGGACTGCCCTGTGGACAAGAGGCGGCGAAACCGCTGCCAGTTCTGCCGCTTCCAGAAGTG
  CCTGGCGGTGGGCATGGTGAAGGAAGTTGTCCGAACAGACAGCCTGAAGGGGCGGCGGGGCCGGCTACCT
  TCAAAACCCAAGCAGCCCCCAGATGCCTCCCCTGCCAATCTCCTCACTTCCCTGGTCCGTGCACACCTGG
  ACTCAGGGCCCAGCACTGCCAAACTGGACTACTCCAAGTTCCAGGAGCTGGTGCTGCCCCACTTTGGGAA
  GGAAGATGCTGGGGATGTACAGCAGTTCTACGACCTGCTCTCCGGTTCTCTGGAGGTCATCCGCAAGTGG
  GCGGAGAAGATCCCTGGCTTTGCTGAGCTGTCACCGGCTGACCAGGACCTGTTGCTGGAGTCGGCCTTCC
  TGGAGCTCTTCATCCTCCGCCTGGCGTACAGGTCTAAGCCAGGCGAGGGCAAGCTCATCTTCTGCTCAGG
  CCTGGTGCTACACCGGCTGCAGTGTGCCCGTGGCTTCGGGGACTGGATTGACAGTATCCTGGCCTTCTCA
  AGGTCCCTGCACAGCTTGCTTGTCGATGTCCCTGCCTTCGCCTGCCTCTCTGCCCTTGTCCTCATCACCG
  ACCGGCATGGGCTGCAGGAGCCGCGGCGGGTGGAGGAGCTGCAGAACCGCATCGCCAGCTGCCTGAAGGA
  GCACGTGGCAGCTGTGGCGGGCGAGCCCCAGCCAGCCAGCTGCCTGTCACGTCTGTTGGGCAAACTGCCC
  GAGCTGCGGACCCTGTGCACCCAGGGCCTGCAGCGCATCTTCTACCTCAAGCTGGAGGACTTGGTGCCCC
  CTCCACCCATCATTGACAAGATCTTCATGGACACGCTGCCCTTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173157 unedited
  AGGATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCGAAACTTG
  GGGGAGTGCACAGAAGAACTTCGGGAGCGCACGCGGGACCAGGGACCAGGCTGAGACTCG
  GGGCGCCAGTCCGGGCAGGGGCAGCGGGAGCCGGCCGGAGATGCCCTGTATCCAAGCCCA
  ATATGGGACACCAGCACCGAGTCCGGGACCCCGTGACCACCTGGCAAGCGACCCCCTGAC
  CCCTGAGTTCATCAAGCCCACCATGGACCTGGCCAGCCCCGAGGCAGCCCCCGCTGCCCC
  CACTGCCCTGCCCAGCTTCAGCACCTTCATGGACGGCTACACAGGAGAGTTTGACACCTT
  CCTCTACCAGCTGCCAGGAACAGTCCAGCCATGCTCCTCAGCCTCCTCCTCGGCCTCCTC
  CACATCCTCGTCCTCAGCCACCTCCCCTGCCTCTGCCTCCTTCAAGTTCGAGGACTTCCA
  GGTGTACGGCTGCTACCCCGGCCCCCTGAGCGGCCCAGTGGATGAGGCCCTGTCCTCCAG
  TGGCTCTGACTACTATGGCAGCCCCTGCTCGGCCCCGTCGCCCTCCACGCNCAGCTTNCA
  GCCGCCCCAGCTCTCTCCCTGGGATGGCTCCTTCGGCCACTTCTCGCCCAGCCAGACTTA
  CGAAGGCCTGCGGGCATGGACAGAGCAGCTGCCCAAGCCTCTGGGGCCCCACAGNCTNCA
  GCCTTCTTTTCTTNCAGTCTNNNCACGGNCCCCAGCCCAGNCTGGNCCAGAGCCNCCTGG
  AGTGTTCCCCTCACAGGCCACCNANCAGCTGGGGNAGGGAGAGAGCTATTTCATGCCCTA
  CGGCTTNNNCAGTTNGNCACCNACTTCTNCACACCTGAGGGCTCGGNGATACTGGNATAC
  ACCGTGACTCAACAGGCCNGNACGNGNCCAGNTGAANTGAGNCGCTGGCTTGGTTGGGCA
  CCCTATGCACATATGTGTCGAATGTAGGCTGCAGGCTCTCAGCGCAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_173157 unedited
  ACGCGGCACGCTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTCCAACTACATGGTCTTATTTTTTTATTCAATTATTATTAATATATTATTATATC
  AGAAAAAGCCACATACTATAAATCCAGGAAAATCCCCAAATATAAAGGTCAAAATCTGTC
  ATTGGTCTTCTTCCTAAAGGGACAGTTTATGTCCATGTGGAAGGAGGGTAGGAAAAAAAA
  CCCAGCAGGAGGCTGGAAAGGCTGCCCAGAAAGGGGGGGCCCATCCTTTCCTCCAAGCCC
  CGTTCCCACCTAAAGCTTTTTCTTCCTGTATTTACAGAAGCACTGGGCTTATTTACAGCA
  CGGCGCTATGGGTTAAAAACGGTTTATCTTACAAAAAACATAAAGGCCGGGGCCAGGCTG
  GGGGGGATAAAACAAATCGGGGGGCCACCCCCATGAAGGCATCCCCCTCCCCAAGGGCTT
  GAGCTCCCTGCAAACCTCCTGGAGCAGGGGAAAAGGGGGAGTCATTCTGCAGCTAAAGCC
  CAGGCTTGGGGGTGCTCTGGGGGTCCGGGGACTAAAGGCACATGGGGGGGCATATGAAAG
  TGCGCATGTGCACACGTGTTCCCAGGCAGGGGTCAAAAGGGCAGCGGGCCCATGAAAATC
  TTGCCAATGATGGGGGGAGGGGGCACCAAGTCCTCCAGCTTGAGGTAAAAGATGCGCTGC
  AGGCCCTGGGTGCACAGGGTCCGCAGTTCGGGCAGTTTGCCCAACAGACGTGACAGCCAC
  CTGGCTGGCTGGGGCTCGCCCGCCAAGTTGCCACGTGCTCCTCAGGCAGCTGGCGATGCG
  GTTTGAAGCTCCTCCCCCGCCGGGGTTCTGAGCCTATGCCGTCGTGATTAGGACAAGCCA
  ATAGCCAGCGATGCAGTACATCAACANCATCTGGCAGGACTTGAAAAGCCGAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_173157
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173157.1, NP_775180.1
• RefSeq Size: 2549 bp
• RefSeq ORF: 1797 bp
• Locus ID: 3164
• Cytogenetics: 12q13.13
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (2) differs in the 5' structure, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 4. The encoded isoform (1) has a shorter N-terminus compared to isoform 3. Variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.