NUR77 (NR4A1) (NM_173157) Human Untagged Clone

CAT#: SC124495

NR4A1 (untagged)-Human nuclear receptor subfamily 4, group A, member 1 (NR4A1), transcript variant 2


  "NM_173157" in other vectors (4)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NR4A1
Synonyms GFRP1; HMR; N10; NAK-1; NGFIB; NP10; NUR77; TR3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_173157, the custom clone sequence may differ by one or more nucleotides


ATGCCCTGTATCCAAGCCCAATATGGGACACCAGCACCGAGTCCGGGACCCCGTGACCACCTGGCAAGCG
ACCCCCTGACCCCTGAGTTCATCAAGCCCACCATGGACCTGGCCAGCCCCGAGGCAGCCCCCGCTGCCCC
CACTGCCCTGCCCAGCTTCAGCACCTTCATGGACGGCTACACAGGAGAGTTTGACACCTTCCTCTACCAG
CTGCCAGGAACAGTCCAGCCATGCTCCTCAGCCTCCTCCTCGGCCTCCTCCACATCCTCGTCCTCAGCCA
CCTCCCCTGCCTCTGCCTCCTTCAAGTTCGAGGACTTCCAGGTGTACGGCTGCTACCCCGGCCCCCTGAG
CGGCCCAGTGGATGAGGCCCTGTCCTCCAGTGGCTCTGACTACTATGGCAGCCCCTGCTCGGCCCCGTCG
CCCTCCACGCCCAGCTTCCAGCCGCCCCAGCTCTCTCCCTGGGATGGCTCCTTCGGCCACTTCTCGCCCA
GCCAGACTTACGAAGGCCTGCGGGCATGGACAGAGCAGCTGCCCAAAGCCTCTGGGCCCCCACAGCCTCC
AGCCTTCTTTTCCTTCAGTCCTCCCACCGGCCCCAGCCCCAGCCTGGCCCAGAGCCCCCTGAAGTTGTTC
CCCTCACAGGCCACCCACCAGCTGGGGGAGGGAGAGAGCTATTCCATGCCTACGGCCTTCCCAGGTTTGG
CACCCACTTCTCCACACCTTGAGGGCTCGGGGATACTGGATACACCCGTGACCTCAACCAAGGCCCGGAG
CGGGGCCCCAGGTGGAAGTGAAGGCCGCTGTGCTGTGTGTGGGGACAACGCTTCATGCCAGCATTATGGT
GTCCGCACATGTGAGGGCTGCAAGGGCTTCTTCAAGCGCACAGTGCAGAAAAACGCCAAGTACATCTGCC
TGGCTAACAAGGACTGCCCTGTGGACAAGAGGCGGCGAAACCGCTGCCAGTTCTGCCGCTTCCAGAAGTG
CCTGGCGGTGGGCATGGTGAAGGAAGTTGTCCGAACAGACAGCCTGAAGGGGCGGCGGGGCCGGCTACCT
TCAAAACCCAAGCAGCCCCCAGATGCCTCCCCTGCCAATCTCCTCACTTCCCTGGTCCGTGCACACCTGG
ACTCAGGGCCCAGCACTGCCAAACTGGACTACTCCAAGTTCCAGGAGCTGGTGCTGCCCCACTTTGGGAA
GGAAGATGCTGGGGATGTACAGCAGTTCTACGACCTGCTCTCCGGTTCTCTGGAGGTCATCCGCAAGTGG
GCGGAGAAGATCCCTGGCTTTGCTGAGCTGTCACCGGCTGACCAGGACCTGTTGCTGGAGTCGGCCTTCC
TGGAGCTCTTCATCCTCCGCCTGGCGTACAGGTCTAAGCCAGGCGAGGGCAAGCTCATCTTCTGCTCAGG
CCTGGTGCTACACCGGCTGCAGTGTGCCCGTGGCTTCGGGGACTGGATTGACAGTATCCTGGCCTTCTCA
AGGTCCCTGCACAGCTTGCTTGTCGATGTCCCTGCCTTCGCCTGCCTCTCTGCCCTTGTCCTCATCACCG
ACCGGCATGGGCTGCAGGAGCCGCGGCGGGTGGAGGAGCTGCAGAACCGCATCGCCAGCTGCCTGAAGGA
GCACGTGGCAGCTGTGGCGGGCGAGCCCCAGCCAGCCAGCTGCCTGTCACGTCTGTTGGGCAAACTGCCC
GAGCTGCGGACCCTGTGCACCCAGGGCCTGCAGCGCATCTTCTACCTCAAGCTGGAGGACTTGGTGCCCC
CTCCACCCATCATTGACAAGATCTTCATGGACACGCTGCCCTTCTGA


>OriGene 5' read for NM_173157 unedited
AGGATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCGAAACTTG
GGGGAGTGCACAGAAGAACTTCGGGAGCGCACGCGGGACCAGGGACCAGGCTGAGACTCG
GGGCGCCAGTCCGGGCAGGGGCAGCGGGAGCCGGCCGGAGATGCCCTGTATCCAAGCCCA
ATATGGGACACCAGCACCGAGTCCGGGACCCCGTGACCACCTGGCAAGCGACCCCCTGAC
CCCTGAGTTCATCAAGCCCACCATGGACCTGGCCAGCCCCGAGGCAGCCCCCGCTGCCCC
CACTGCCCTGCCCAGCTTCAGCACCTTCATGGACGGCTACACAGGAGAGTTTGACACCTT
CCTCTACCAGCTGCCAGGAACAGTCCAGCCATGCTCCTCAGCCTCCTCCTCGGCCTCCTC
CACATCCTCGTCCTCAGCCACCTCCCCTGCCTCTGCCTCCTTCAAGTTCGAGGACTTCCA
GGTGTACGGCTGCTACCCCGGCCCCCTGAGCGGCCCAGTGGATGAGGCCCTGTCCTCCAG
TGGCTCTGACTACTATGGCAGCCCCTGCTCGGCCCCGTCGCCCTCCACGCNCAGCTTNCA
GCCGCCCCAGCTCTCTCCCTGGGATGGCTCCTTCGGCCACTTCTCGCCCAGCCAGACTTA
CGAAGGCCTGCGGGCATGGACAGAGCAGCTGCCCAAGCCTCTGGGGCCCCACAGNCTNCA
GCCTTCTTTTCTTNCAGTCTNNNCACGGNCCCCAGCCCAGNCTGGNCCAGAGCCNCCTGG
AGTGTTCCCCTCACAGGCCACCNANCAGCTGGGGNAGGGAGAGAGCTATTTCATGCCCTA
CGGCTTNNNCAGTTNGNCACCNACTTCTNCACACCTGAGGGCTCGGNGATACTGGNATAC
ACCGTGACTCAACAGGCCNGNACGNGNCCAGNTGAANTGAGNCGCTGGCTTGGTTGGGCA
CCCTATGCACATATGTGTCGAATGTAGGCTGCAGGCTCTCAGCGCAGG
>OriGene 3' read for NM_173157 unedited
ACGCGGCACGCTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTCCAACTACATGGTCTTATTTTTTTATTCAATTATTATTAATATATTATTATATC
AGAAAAAGCCACATACTATAAATCCAGGAAAATCCCCAAATATAAAGGTCAAAATCTGTC
ATTGGTCTTCTTCCTAAAGGGACAGTTTATGTCCATGTGGAAGGAGGGTAGGAAAAAAAA
CCCAGCAGGAGGCTGGAAAGGCTGCCCAGAAAGGGGGGGCCCATCCTTTCCTCCAAGCCC
CGTTCCCACCTAAAGCTTTTTCTTCCTGTATTTACAGAAGCACTGGGCTTATTTACAGCA
CGGCGCTATGGGTTAAAAACGGTTTATCTTACAAAAAACATAAAGGCCGGGGCCAGGCTG
GGGGGGATAAAACAAATCGGGGGGCCACCCCCATGAAGGCATCCCCCTCCCCAAGGGCTT
GAGCTCCCTGCAAACCTCCTGGAGCAGGGGAAAAGGGGGAGTCATTCTGCAGCTAAAGCC
CAGGCTTGGGGGTGCTCTGGGGGTCCGGGGACTAAAGGCACATGGGGGGGCATATGAAAG
TGCGCATGTGCACACGTGTTCCCAGGCAGGGGTCAAAAGGGCAGCGGGCCCATGAAAATC
TTGCCAATGATGGGGGGAGGGGGCACCAAGTCCTCCAGCTTGAGGTAAAAGATGCGCTGC
AGGCCCTGGGTGCACAGGGTCCGCAGTTCGGGCAGTTTGCCCAACAGACGTGACAGCCAC
CTGGCTGGCTGGGGCTCGCCCGCCAAGTTGCCACGTGCTCCTCAGGCAGCTGGCGATGCG
GTTTGAAGCTCCTCCCCCGCCGGGGTTCTGAGCCTATGCCGTCGTGATTAGGACAAGCCA
ATAGCCAGCGATGCAGTACATCAACANCATCTGGCAGGACTTGAAAAGCCGAAC
Restriction Sites NotI-NotI     
ACCN NM_173157
Insert Size 2550 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_173157.1, NP_775180.1
RefSeq Size 2549 bp
RefSeq ORF 1797 bp
Locus ID 3164
Cytogenetics 12q13.13
Protein Families Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
Protein Pathways MAPK signaling pathway
Gene Summary 'This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'
Transcript Variant: This variant (2) differs in the 5' structure, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 4. The encoded isoform (1) has a shorter N-terminus compared to isoform 3. Variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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