EEF1A1 (NM_001402) Human Untagged Clone

CAT#: SC124558

EEF1A1 (untagged)-Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EEF1A1
• Synonyms: CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EF-Tu; EF1A; GRAF-1EF; HNGC:16303; LENG7; PTI1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124558 sequence for NM_001402 edited (data generated by NextGen Sequencing)
  ATGGGAAAGGAAAAGACTCATATCAACATTGTCGTCATTGGACACGTAGATTCGGGCAAG
  TCCACCACTACTGGCCATCTGATCTATAAATGCGGTGGCATCGACAAAAGAACCATTGAA
  AAATTTGAGAAGGAGGCTGCTGAGATGGGAAAGGGCTCCTTCAAGTATGCCTGGGTCTTG
  GATAAACTGAAAGCTGAGCGTGAACGTGGTATCACCATTGATATCTCCTTGTGGAAATTT
  GAGACCAGCAAGTACTATGTGACTATCATTGATGCCCCAGGACACAGAGACTTTATCAAA
  AACATGATTACAGGGACATCTCAGGCTGACTGTGCTGTCCTGATTGTTGCTGCTGGTGTT
  GGTGAATTTGAAGCTGGTATCTCCAAGAATGGGCAGACCCGAGAGCATGCCCTTCTGGCT
  TACACACTGGGTGTGAAACAACTAATTGTCGGTGTTAACAAAATGGATTCCACTGAGCCA
  CCCTACAGCCAGAAGAGATATGAGGAAATTGTTAAGGAAGTCAGCACTTACATTAAGAAA
  ATTGGCTACAACCCCGACACAGTAGCATTTGTGCCAATTTCTGGTTGGAATGGTGACAAC
  ATGCTGGAGCCAAGTGCTAACATGCCTTGGTTCAAGGGATGGAAAGTCACCCGTAAGGAT
  GGCAATGCCAGTGGAACCACGCTGCTTGAGGCTCTGGACTGCATCCTACCACCAACTCGT
  CCAACTGACAAGCCCTTGCGCCTGCCTCTCCAGGATGTCTACAAAATTGGTGGTATTGGT
  ACTGTTCCTGTTGGCCGAGTGGAGACTGGTGTTCTCAAACCCGGTATGGTGGTCACCTTT
  GCTCCAGTCAACGTTACAACGGAAGTAAAATCTGTCGAAATGCACCATGAAGCTTTGAGT
  GAAGCTCTTCCTGGGGACAATGTGGGCTTCAATGTCAAGAATGTGTCTGTCAAGGATGTT
  CGTCGTGGCAACGTTGCTGGTGACAGCAAAAATGACCCACCAATGGAAGCAGCTGGCTTC
  ACTGCTCAGGTGATTATCCTGAACCATCCAGGCCAAATAAGCGCCGGCTATGCCCCTGTA
  TTGGATTGCCACACGGCTCACATTGCATGCAAGTTTGCTGAGCTGAAGGAAAAGATTGAT
  CGCCGTTCTGGTAAAAAGCTGGAAGATGGCCCTAAATTCTTGAAGTCTGGTGATGCTGCC
  ATTGTTGATATGGTTCCTGGCAAGCCCATGTGTGTTGAGAGCTTCTCAGACTATCCACCT
  TTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGGTGTCATCAAAGCA
  GTGGACAAGAAGGCTGCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAG
  GCTAAATGA
  
  Clone variation with respect to NM_001402.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001402 unedited
  NTTCGGAATTGAAACCGATTACTATAGGCGGACGCCCAATTCGCACGAGGGCCGCCATAC
  ACAGGTGTCGTGAAACTACCCCCAAAGCCAAAAGGGAAAGGAAAAGACTCATATCAACAT
  TGTCGTCATTGGACACGTAGATTCGGGCAAGTCCACCACTACTGGCCATCTGATCTATAA
  ATGCGGTGGCATCGACAAAAGATTTTTTGTAAAAATTTGAGAAGGAGGCTGCTGAGATGG
  GAAAGGGCTCCTTCAAGTATGCCTGGGTCTTGGATAAACTGAAAGCTGAGCGTGAACGTG
  GTATCACCATTGATATCTCCTTGTGGAAATTTGAGACCAGCAAGTACTATGTGACTATCA
  TTGATGCCCCAGGACACAGAGACTTTATCAAAAACATGATTACAGGGACATCTCAGGCTG
  ACTGTGCTGTCCTGATTGTTGCTGCTGGTGTTGGTGAATTTGAAGCTGGTATCTCCAAGA
  ATGGGCAGACCCGAGAGCATGCCCTTCTGGCTTACACACTGGGTGTGAAACAACTAATTG
  TCGGTGTTAACAAAATGGATTCCACTGAGCCACCCTACAGCCAGAAGAGATATGAGGAAA
  TTGTTAAGGAAGTCAGCACTTACATTAAGAAAATTGGCTACAACCCCGACACAGTAGCAT
  TTGTGCCAATTTCTGGTTGGAATGGTGACAACATGCTGGAGCCAAGTGCTAACATGCCTT
  GGTTCAAGGGATGGAAAGTCACCCGTAAAGATGGCCATGCCAGTGGAAACACGCCTGCTT
  GAGCTCTGGACTGCATCCTACCACCAACTCGTCCCACTGACAAGCCCCTTGCGCCCTGCC
  TCTCCAGGATGTCTACAAAATTGGTGGCNATTGGTACTGTTTCCTGTTGGNCCGAGTGAA
  AACTGCNTGTCTTCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001402 unedited
  ATGGCAACTTTCCAGGNCCAGGNANAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGG
  ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
  TTTTTCTCATTTAACTTTTTTAATGGGTCTCAAAATTCTGTGACAAATTTTTGGTCAAGT
  TGTTTCCATTAAAAAGTACTGATTTTAAAAACTAATAACTTAAAACTGCCACACGCAAAA
  AAGAAAACCAAAGTGGTCCACAAAACATTCTCCTTTCCTTCTGAAGGTTTTACGATGCAT
  TGTTATCATTAACCAGTCTTTTACTACTAAACTTAAATGGCCAATTGAAACAAACAGTTC
  TGAGACCGTTCTTCCACCACTGATTAAGAGTGGGGTGGCAGGTATTAGGGATAATATTCA
  TTTAGCCTTCTGAGCTTTCTGGGCAGACTTGGTGACCTTGCCAGCTCCAGCAGCCTTCTT
  GTCCACTGCTTTGATGACACCCACCGCAACTGTCTGTCTCATATCACGAACAGCAAAGCG
  ACCCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGNGGCTTGCCAGGAACCATAT
  CAACAATGGCAGCATCACCAGACTTCAAGAATTTAGGGCCATCTTCCAGCTTTTTACCAG
  AACGGCGATCAATCTTTTCCTTCAGCTCAGCAAACTTGCATGCAAATGTGAGCCGTGTGG
  CAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCANGATAATCACC
  TGAGCAGTGAAGCAGCTGCTCCATTGGTGGGTCATTTTTGCTGTCACCAGCACGTGCCAC
  GACGACATCCTTGCAGACACATTCTGACATGAGCCCACATTGTCCCAGNA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001402
• Insert Size: 1710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001402.4, NP_001393.1
• RefSeq Size: 1837 bp
• RefSeq ORF: 1389 bp
• Locus ID: 1915
• Cytogenetics: 6q13
• Domains: GTP_EFTU, GTP_EFTU_D3, GTP_EFTU_D2
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]'