MRPL54 (NM_172251) Human Untagged Clone

CAT#: SC124635

MRPL54 (untagged)-Human mitochondrial ribosomal protein L54 (MRPL54), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL54
• Synonyms: L54mt; MRP-L54
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124635 sequence for NM_172251 edited (data generated by NextGen Sequencing)
  ATGGCGACCAAACGCCTTTTCGGGGCTACCCGGACGTGGGCCGGCTGGGGGGCCTGGGAG
  CTCCTAAACCCCGCCACTTCCGGAAGACTCCTGGCCCGGGATTATGCCAAGAAACCAGTT
  ATGAAGGGGGCCAAATCGGGAAAAGGTGCAGTGACCAGCGAGGCCCTCAAGGACCCCGAC
  GTATGCACAGATCCTGTCCAGCTCACCACATATGCCATGGGCGTCAACATCTACAAGGAA
  GGGCAGGATGTACCCCTGAAACCGGATGCTGAGTACCCTGAATGGCTGTTTGAGATGAAC
  TTGGGTCCCCCAAAGACCCTGGAGGAGCTGGACCCCGAGAGCCGGGAGTACTGGCGGCGG
  CTGCGGAAACAGAACATCTGGCGCCACAACCGGCTGAGCAAGAACAAGAGGTTGTAG
  
  Clone variation with respect to NM_172251.2
  291 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172251 unedited
  GTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCAATACGTTA
  TGGCGACCAAACGCCTTTTCGGGGCTACCCGGACGTGGGCCGGCTGGGGGGCCTGGGAGC
  TCCTAAACCCCGCCACTTCCGGAAGACTCCTGGCCCGGGATTATGCCAAGAAACCAGTTA
  TGAAGGGGGCCAAATCGGGAAAAGGTGCAGTGACCAGCGAGGCCCTCAAGGACCCCGACG
  TATGCACAGATCCTGTCCAGCTCACCACATATGCCATGGGCGTCAACATCTACAAGGAAG
  GGCAGGATGTACCCCTGAAACCGGATGCTGAGTACCCTGAATGGCTGTTTGAGATGAACT
  TGGGTCCCCCAAAGACCCTGGATGATCTGGACCCCGATAGCCGGGAGTACTGGCGGCGGC
  TGCGGAAACAGAACATCTGGCGCCACAACCGGCTGAGCAAGAACAAGATGTTGTAGCATG
  GAGGGCCCGGCATCGCTGACCCCCACGCCGATGGCTTGCCGTTTTCCCGGAGGACGTGGA
  CTTTTGTGAGACAAGATGCGGCTCCCCAGCCTGGGTTTCCATGTGACCCCACAGTGGGGG
  CTGGACCATGGCCCTGGAGGCCAATAAAGAGCTTTCTAGGGTAGACCCTANNNAAAAAAA
  AAAAANNAAAAAAAAAAAAANNAAACCTCGACTCTAGATTGCGGCCGCGGTCATTAGCTG
  TTTCCTGACAATCCCCCGGTGGCATCCCTGTGACCCCTCCCCATGCCTCTCTGCNCCTGG
  GGATGTTGCCACTCAGTGCCCACCAGCCTGCCTATAAAAATAAGTGCATCATTTGCTGAC
  TAGGGGCTATAATATGGGNNGGGGNGGGGGGGGGGGGTGGNNNNNAGGAGCAGGGNNNNN
  AAGAAAAACACCGGAGGCCCGGGGGTCTTAGAAACAAACTGGAATGCATGCACATCTGGC
  TACTGATCTCGCTCCGGGTTAACGATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_172251
• ORF Size: 417 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_172251.1, NP_758455.1
• RefSeq Size: 615
• RefSeq ORF: 417
• Locus ID: 116541
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]