RAB27A (NM_183235) Human Untagged Clone

CAT#: SC124653

RAB27A (untagged)-Human RAB27A, member RAS oncogene family (RAB27A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB27A
• Synonyms: GS2; HsT18676; RAB27; RAM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124653 sequence for NM_183235 edited (data generated by NextGen Sequencing)
  ATGTCTGATGGAGATTATGATTACCTCATCAAGTTTTTAGCTTTGGGAGACTCTGGTGTA
  GGGAAGACCAGTGTACTTTACCAATATACAGATGGTAAATTTAACTCCAAATTTATCACA
  ACAGTGGGCATTGATTTCAGGGAAAAAAGAGTGGTGTACAGAGCCAGTGGGCCGGATGGA
  GCCACTGGCAGAGGCCAGAGAATCCACCTGCAGTTATGGGACACAGCAGGGCAGGAGAGG
  TTTCGTAGCTTAACGACAGCGTTCTTCAGAGATGCTATGGGTTTTCTTCTACTTTTTGAT
  CTGACAAATGAGCAAAGTTTCCTCAATGTCAGAAACTGGATAAGCCAGCTACAGATGCAT
  GCATATTGTGAAAACCCAGATATAGTGCTGTGTGGAAACAAGAGTGATCTGGAGGACCAG
  AGAGTAGTGAAAGAGGAGGAAGCCATAGCACTCGCAGAGAAATATGGAATCCCCTACTTT
  GAAACTAGTGCTGCCAATGGGACAAACATAAGCCAAGCAATTGAGATGCTTCTGGACCTG
  ATAATGAAGCGAATGGAACGGTGTGTGGACAAGTCCTGGATTCCTGAAGGAGTGGTGCGA
  TCAAATGGTCATGCCTCTACGGATCAGTTAAGTGAAGAAAAGGAGAAAGGGGCATGTGGC
  TGTTGA
  
  Clone variation with respect to NM_183235.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_183235 unedited
  CAGGATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGCAACGACCC
  AAAAAAGCCGGCGGCGGGTGGACGCGGCGCGCGAGGACCCGAGCCAGGCGGGGACAGGAC
  GGCGGGGCGAGCCAGACAAAAAGTAACCTTCCTGAAGAGGACATGTGATTGGAAGTTGTC
  AATTGTTGAAGCATTGGTAACTCCAGTCTCTAACGTTTTAGAAAATCATAACAAGCGGTT
  CTCTACCCTGTAAAGGTGAACTACTGAGTTCTTCATTATGTCTGATGGAGATTATGATTA
  CCTCATCAAGTTTTTAGCTTTGGGAGACTCTGGTGTAGGGAAGACCAGTGTACTTTACCA
  ATATACAGATGGTAAATTTAACTCCAAATTTATCACAACAGTGGGCATTGATTTCAGGGA
  AAAAAGAGTGGTGTACAGAGCCAGTGGGCCGGATGGAGCCACTGGCAGAGGCCAGAGAAT
  CCACCTGCAGTTATGGGACACAGCAGGGCAGGAGAGGTTTCGTAGCTTAACGACAGCGTT
  CTTCAGAGATGCTATGGGTTTTCTTCTACTTTTTGATCTGACAAATGAGCAAAGTTTCCT
  CAATGTCAGAAACTGGATAAGCCAGCTACAGATGCATGCATATTGTGAAAACCCAGATAT
  AGTGCTGTGTGGAAACAAGAGTGATCTGGAGGACCAGAGAGTAGTGAAAGAGGAGGAAGC
  CATAGCACTCGCAGAGAAATATGGAATCCCCTACTTTGAAACTAGTGCTGCCAATGGGAC
  ANACATNAAGCCAAGCATTGAGATGCTTCTGGACCTGATATGAAGCGATGGAACGGTGTG
  TGGACCAGTCTGGATTCTGAAGGATGGTGCGATCAATGTCATGCTCTACGGATCAGTAAT
  GAAAAAAGAAAAGGGGCATGTGCTGTTGAAAATCAGTAGCGACTATANNTCAGTGGCCAT
  GCCTGGATCTCCTATGATGGCATGCCATGAAAC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_183235 unedited
  NCCCTGATAAACATNAACCCAAGCATTGTANAGCTTCTGGNACTGGAAATGAAGCGAATG
  GAACGGTGTGTGGACAAGTCCTGGCATCCTGAAGGAGTGGTGCGATCAAATGGTCATGCC
  TCTACGGATCAGTTAAGTGAAGAAAAGGAGAAAGGGGCATGTGGCTGTTGAGAAGTCAAG
  TAAGCGACATAGTAGTTCAGGTGGCCCATGCCTGGGATCTTCTCTATGATTGGTACATGG
  CACAGTGAGAGATTAATGGGCATTGTGTACAAATTGCTTCTCACCATCCCCATTAGACCT
  ACGAATAAAGCATCCGGTTCTAAAATTAATTTGTTGCAGCTTTGTAAATATTTCTTTAAG
  ATTCAGCCTGAGAGTTAGGAGAAATATTTCAGAGCCAAAAGTGCCTTATACAACCTTAGC
  CTATTATAGTAAATCATTCAAGGATTCAGAATTTTGCAGTCACAGAAGAGTGTATTTATT
  ATGTAGAATGAATGAGGGTACTGTCACCTGCCTTAATGTAGGTAGGCCCAGAGTCTTACA
  TTTAAGATCTTACATGCAGTTATAAAACCGCCACAGTCTTCAATCCAGATTTGAAGACTC
  ATGCCATAGGTGACATTCTAAAATACCATTAAAGCCACTTAAATGTTAAATAAGAATATA
  CATGCACATCAGCTCAATGTCTTTGAGTAATAAAATTTATGTAAGCATTCTTATTAACAT
  GAATTTTGGACAAATCATGGGTATTTCTATAGACCCTGGGTAAACTGGGTTGACCCATTT
  TTCCCTCACGGAGACTTTTATTATGGTGGGAGGGGGAATGTAGTGGAGCAGGCGAGGAGN
  AGAAACTCGACCAAGGGTGGGCAAGGAAGATAAAATAAACAAGTGTGAGGTAGGTGCGGT
  GACGGAGGGAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_183235
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183235.1, NP_899058.1
• RefSeq Size: 2809 bp
• RefSeq ORF: 666 bp
• Locus ID: 5873
• Cytogenetics: 15q21.3
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.