FYB1 (NM_199335) Human Untagged Clone

CAT#: SC124654

FYB (untagged)-Human FYN binding protein (FYB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FYB1
• Synonyms: ADAP; FYB; PRO0823; SLAP-130; SLAP130; THC3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_199335, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGAAATATAACACGGGGGGCAACCCGACAGAGGATGTCTCAGTCAATAGCCGACCCTTCAGAGTCA
  CAGGGCCAAACTCATCTTCAGGAATACAAGCAAGAAAGAACTTATTCAACAACCAAGGAAATGCCAGCCC
  TCCTGCAGGACCCAGCAATGTACCTAAGTTTGGGTCCCCAAAGCCACCTGTGGCAGTCAAACCTTCTTCT
  GAGGAAAAGCCTGACAAGGAACCCAAGCCCCCGTTTCTAAAGCCCACTGGAGCAGGCCAAAGATTCGGAA
  CACCAGCCAGCTTGACCACCAGAGACCCCGAGGCGAAAGTGGGATTTCTGAAACCTGTAGGCCCCAAGCC
  CATCAACTTGCCCAAAGAAGATTCCAAACCTACATTTCCCTGGCCTCCTGGAAACAAGCCATCTCTTCAC
  AGTGTAAACCAAGACCATGACTTAAAGCCACTAGGCCCGAAATCTGGGCCTACTCCTCCAACCTCAGAAA
  ATGAACAGAAGCAAGCGTTTCCCAAATTGACTGGGGTTAAAGGGAAATTTATGTCAGCATCACAAGATCT
  TGAACCCAAGCCCCTCTTCCCCAAACCCGCCTTTGGCCAGAAGCCGCCCCTAAGTACCGAGAACTCCCAT
  GAAGACGAAAGCCCCATGAAGAATGTGTCTTCATCAAAAGGGTCCCCAGCTCCCCTGGGAGTCAGGTCCA
  AAAGCGGCCCTTTAAAACCAGCAAGGGAAGACTCAGAAAATAAAGACCATGCAGGGGAGATTTCAAGTTT
  GCCCTTTCCTGGAGTGGTTTTGAAACCTGCTGCGAGCAGGGGAGGCCCAGGTCTCTCCAAAAATGGTGAA
  GAAAAAAAGGAAGATAGGAAGATAGATGCTGCTAAGAACACCTTCCAGAGCAAAATAAATCAGGAAGAGT
  TGGCCTCAGGGACTCCTCCTGCCAGGTTCCCTAAGGCCCCTTCTAAGCTGACAGTGGGGGGGCCATGGGG
  CCAAAGTCAGGAAAAGGAAAAGGGAGACAAGAATTCAGCCACCCCGAAACAGAAGCCATTGCCTCCCTTG
  TTTACCTTGGGTCCACCTCCACCAAAACCCAACAGACCACCAAATGTTGACCTGACGAAATTCCACAAAA
  CCTCTTCTGGAAACAGTACTAGCAAAGGCCAGACGTCTTACTCAACAACTTCCCTGCCACCACCTCCACC
  ATCCCATCCGGCCAGCCAACCACCATTGCCAGCATCTCACCCATCACAACCACCAGTCCCAAGCCTACCT
  CCCAGAAACATTAAACCTCCGTTTGACCTAAAAAGCCCTGTCAATGAAGACAATCAAGATGGTGTCACGC
  ACTCTGATGGTGCTGGAAATCTAGATGAGGAACAAGACAGTGAAGGAGAAACATATGAAGACATAGAAGC
  ATCCAAAGAAAGAGAGAAGAAAAGGGAAAAGGAAGAAAAGAAGAGGTTAGAGCTGGAGAAAAAGGAACAG
  AAAGAGAAAGAAAAGAAAGAACAAGAAATAAAGAAGAAATTTAAACTAACAGGCCCTATTCAAGTCATCC
  ATCTTGCAAAAGCTTGTTGTGATGTCAAAGGAGGAAAGAATGAACTGAGCTTCAAGCAAGGAGAGCAAAT
  TGAAATCATCCGCATCACAGACAACCCAGAAGGAAAATGGTTGGGCAGAACAGCAAGGGGTTCATATGGC
  TATATTAAAACAACTGCTGTAGAGATTGACTATGATTCTTTGAAACTGAAAAAAGACTCTCTTGGTGCCC
  CTTCAAGACCTATTGAAGATGACCAAGAAGTATATGATGATGTTGCAGAGCAGGATGATATTAGCAGCCA
  CAGTCAGAGTGGAAGTGGAGGGATATTCCCTCCACCACCAGATGATGACATTTATGATGGGATTGAAGAG
  GAAGATGCTGATGATGGTTTCCCTGCTCCTCCTAAACAATTGGACATGGGAGATGAAGTTTACGATGATG
  TGGATACCTCTGATTTCCCTGTTTCATCAGCAGAGATGAGTCAAGGAACTAATGTTGGAAAAGCTAAGAC
  AGAAGAAAAGGACCTTAAGAAGCTAAAAAAGCAGGAAAAAGAAGAAAAAGACTTCAGGAAAAAATTTAAA
  TATGATGGTGAAATTAGAGTCCTATATTCAACTAAAGTTACAACTTCCATAACTTCTAAAAAGTGGGGAA
  CCAGAGATCTACAGGTAAAACCTGGTGAATCTCTAGAAGTTATACAAACCACAGATGACACAAAAGTTCT
  CTGCAGAAATGAAGAAGGGAAATATGGTTATGTCCTTCGGAGTTACCTAGCGGACAATGATGGAGAGATC
  TATGATGATATTGCTGATGGCTGCATCTATGACAATGACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_199335 unedited
  NGGTCAGGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCATCTTCG
  GGGTCATTGTGTGCCAGGCATATCAACTCTTTTCAATAAAAAGGATGGAAAGGCAGATGT
  AAAGTCCCTCATGGCGAAATATAACACGGGGGGCAACCCGACAGAGGATGTCTCAGTCAA
  TAGCCGACCCTTCAGAGTCACAGGGCCAAACTCATCTTCAGGAATACAAGCAAGAAAGAA
  CTTATTCAACAACCAAGGAAATGCCAGCCCTCCTGCAGGACCCAGCAATGTACCTAAGTT
  TGGGTCCCCAAAGCCACCTGTGGCAGTCAAACCTTCTTCTGAGGAAAAGCCTGACAAGGA
  ACCCAAGCCCCCGTTTCTAAAGCCCACTGGAGCAGGCCAAAGATTCGGAACACCAGCCAG
  CTTGACCACCAGAGACCCCGAGGCGAAAGTGGGATTTCTGAAACCTGTAGGCCCCAAGCC
  CATCAACTTGCCCAAAGAAGATTCCAAACCTACATTTCCCTGGCCTCCTGGAAACAAGCC
  ATCTCTTCACAGTGTAAACCAAGACCATGACTTAAAGCCACTAGGCCCGAAATCTGGGCC
  TACTCCTCCAACCTCAGAAAATGAACAGAAGCAAGCGTTTCCCAAATTGACTGGGGTTAA
  AGGGAAATTTATGTCAGCATCACAAGATCTTGAACCCAAGCCCCTCTTCCCCAAACCCGC
  CTTTGGCCAGAAGCCGCCCCTAAGTACCGAGAACTCCCATGAAGACGAAAGCCCCATGAA
  GAATGTGTCTTCATCAAAAGGGTCCCNCAGCTCCCTGNGAGTCANGTCCAAAGCGGNCCT
  TTAAAACCAGCAGGAAGACTCANAAATAAAGACATGCAGGGAGATTTCAGNTTGCCCTTT
  CTGGAGTGTTTTGAACCTGCNTGCACAGGGGAGCCCAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_199335 unedited
  GCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTATCCATTGAAGGT
  ATTATTTATTTGCAGCTCATCTTAAGTGACAAAATTCCATACAGAAGACTATAACAGAAA
  TCATATTTAATATATTAAAATTAATACTTCAAATATCTTTCACATTAAGATGATTATCTA
  TTGTGTAAATCTTTCCTAGGTATGTGTGTCTGTTTCTTGATGTGTAAACCAAAACTCTGA
  AATATTCTCTTGATCTAACTTTGACTTTTAAAAACTGACATTGTACTGAATTTACATAAT
  TCTCAATCAGAAAAAAAATTACTGTCAGACTGCAATGCAAGTCTGCCCCAATGAAGGCAG
  GAGGAGGGATGGCTGGAAGTGTCTCAGGGCACTCTGAAAGTTTAGCAAGCTTATCAAGGA
  GTCCTCAAACTAAAGCTGGCCAAAAACTTCAGCATAAAAACTATCCTGTCTGTGTATTAT
  ATATATTTATTACCAAATTTAATAAACAAAAACCACTTTTGAACCAGGTAATTTTATCTA
  GTGTGAAAAGAAATTAATCACATGGTCATATACTGGCTAGTGCTCTCTAAAAGTAGACAT
  TAAAATATTTCATAATTGGAGGGAATCTTTGGAGATTAGTGGCATCTAATCTTGGGGCCT
  CAGACACCCAAAATCTATATATTGACCAGTGGAAGTTGATGATTTGTTATCAAATTTCAA
  AGCACCTAATGGAAACCCCACTTCATCTCTGTGAAGATTTTCAGTTCCNTTACAGTTATT
  TGAAAATATTTCAATTCTGTGGAGCCACTTCAGAAAACTCCATGGGCCNTTTGCATTTAA
  AATGTGGGAGATGCATTAATCATTATTTAATNNCATGAGTTAAAAAATAGACAAAGCTTC
  TGAANCATGGGACTGNAGTANNGCGGAGCATATTAAAAGCCTAANGGTATTGGTANTGAA
  AAGTGGGAAAAACTTACTAGTCTAATATTGTNANTTACGAATAGGNAACTCGAAAGAAAA
  ATATTTTTCCATTACACTCGAGGTTCATTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_199335
• Insert Size: 4130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199335.2, NP_955367.1
• RefSeq Size: 4738 bp
• RefSeq ORF: 2352 bp
• Locus ID: 2533
• Cytogenetics: 5p13.1
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is an adapter for the FYN protein and LCP2 signaling cascades in T-cells. The encoded protein is involved in platelet activation and controls the expression of interleukin-2. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (2) differs in the 5' UTR, uses a downstream start codon, and lacks an alternate in-frame exon in the 3' coding region compared to variant 3. The resulting isoform (2) has a shorter N-terminus and lacks an internal segment compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.