ASS1 (NM_054012) Human Untagged Clone

CAT#: SC124908

ASS1 (untagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASS1
• Synonyms: ASS; CTLN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124908 sequence for NM_054012 edited (data generated by NextGen Sequencing)
  ATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGACACCTCGTGCATC
  CTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGCCAACATTGGCCAG
  AAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGGGGCCAAAAAGGTGTTC
  ATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCCGGCCATCCAGTCCAGC
  GCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAGGCCCTGCATCGCCCGC
  AAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCACGGCGCCACAGGA
  AAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACTGGCCCCCCAGATAAAG
  GTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAAGGGCCGCAATGACCTG
  ATGGAGTACGCAAAGCAACACGGGATTCCCATCCCGGTCACTCCCAAGAACCCGTGGAGC
  ATGGATGAGAACCTCATGCACATCAGCTACGAGGCTGGAATCCTGGAGAACCCCAAGAAC
  CAAGCGCCTCCAGGTCTCTACACGAAGACCCAGGACCCAGCCAAAGCCCCCAACACCCCT
  GACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGAAGGTGACCAACGTCAAGGAT
  GGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAACGAAGTCGCGGGCAAG
  CATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT
  ATCTACGAGACCCCAGCAGGCACCATCCTTTACCATGCTCATTTAGACATCGAGGCCTTC
  ACCATGGACCGGGAAGTGCGCAAAATCAAACAAGGCCTGGGCTTGAAATTTGCTGAGCTG
  GTGTATACCGGTTTCTGGCACAGCCCTGAGTGTGAATTTGTCCGCCACTGCATCGCCAAG
  TCCCAGGAGCGAGTGGAAGGGAAAGTGCAGGTGTCCGTCCTCAAGGGCCAGGTGTACATC
  CTCGGCCGGGAGTCCCCACTGTCTCTCTACAATGAGGAGCTGGTGAGCATGAACGTGCAG
  GGTGATTATGAGCCAACTGATGCCACCGGGTTCATCAACATCAATTCCCTCAGGCTGAAG
  GAATATCATCGTCTCCAGAGCAAGGTCACTGCCAAATAG
  
  Clone variation with respect to NM_054012.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_054012 unedited
  TCACAATTTNGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCCACCGCT
  GCCCGAGCCCGACGCTATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCT
  GGACACCTCGTGCATCCTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCT
  GGCCAACATTGGCCAGAAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGG
  GGCCAAAAAGGTGTTCATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCC
  GGCCATCCAGTCCAGCGCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAG
  GCCCTGCATCGCCCGCAAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTC
  CCACGGCGCCACAGGAAAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACT
  GGCCCCCCAGATAAAGGTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAA
  GGGCCGCAATGACCTGATGGAGTACGCAAAGCAACACGGGATTCCCATCCCGGTCACTCC
  CAAGAACCCGTGGAGCATGGATGAGAACCTCATGCACATCAGCTACGAGGCTGGAATCCT
  GGAGAACCCCAAGAACCAAGCGCCTCCAGGTCTCTACACGAAGACCCAGGACCCAGCCAA
  AGCCCCCAACACCCCTGACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGANAGT
  GACCAACGTCAAGGATGGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAA
  CGAAGTCGCGGGCAAGCATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTTCATGG
  NAATGAGTCCCGAGTATCTACGAGACCCAGCAGGCACATCCTTTACATGCTCATTTAGAC
  TCGAGCCTTCACCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_054012 unedited
  CTTTGGTACGTAGCCGCATACTAGGTTCAGTTTTATTTTTTTTTTTTTTTTTTTTTTTCT
  CTTTTAATTGTCATTTTTATAGCTCCCCACCGCAGCTGCCCCCCACCCTTCCCTTCGATG
  ACAACGTTTGCAGGCTTCAGGGGGACCAGGGAACAAAGCTGGGGCCTGGCAGCCCCACTA
  CGCTGCCAGCCGGGGAGAACAAGTCACAATTACTAATTATCACAACAATTAGCGCCTGTA
  CTTGGGGGATCTGCAAATTGAGGAGGCCCCAGCTCCTCATTGTACACGGGTCTATTTGGC
  AGTGACCTTGCTCTGGAGACGATGATATTCCTTCAGCCTGAGGGAATTGATGTTGATGAA
  CCCGGTGGCATCAGTTGGCTCATAATCACCCTGCACGTTCATGCTCACCAGCTCCTCATT
  GTAGAGAGACAGTGGGGACTCCCGGCCGAGGATGTACACCTGGCCCTTGAGGACGGACAC
  CTGCACTTTCCCTTCCACTCGCTCCTGGGACTTGGCGATGCAGTGGCGGACAAATTCACA
  CTCAGGGCTGTGCCAGAAACCGGTATACACCAGCTCAGCAAATTTCAAGCCCAGGCCTTG
  TTTGATTTTGCGCACTTCCCGGTCCATGGTGAAGGCCTCGATGTCTAAATGAGCATGGTA
  AAGGATGGTGCCTGCTGGGGTCTCGTAGATACCTCGGGACTTCATTCCAATGAAGCGGTT
  CTCCACGATGTCAATACGGCCCACGCCATGCTTGCCCGCGACTTCGTTCAGGTACATGAA
  GAGCTCCAAGGAGTCTGGTGGGTGGTGCCATCCTTGACGTTGGTCACCTTCCAGGGACCC
  CTTTTTTTGACTCGATCTCNAGAATGTCAGGNGTGTTNGGGGGCTTTGGCTGGGTCCTGG
  GTCTTCGTGTANAGACCTGGAGGCGCTTGGGTCTNGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_054012
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_054012.2, NP_446464.1
• RefSeq Size: 1631 bp
• RefSeq ORF: 1239 bp
• Locus ID: 445
• Cytogenetics: 9q34.11
• Domains: Arginosuc_synth
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012]'
  Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.