Repulsive Guidance Molecule C (HFE2) (NM_145277) Human Untagged Clone

CAT#: SC124916

HFE2 (untagged)-Human hemochromatosis type 2 (juvenile) (HFE2), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HFE2
• Synonyms: HFE2; HFE2A; JH; RGMC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124916 sequence for NM_145277 edited (data generated by NextGen Sequencing)
  ATGATCCAGCACAACTGCTCCCGCCAGGGCCCTACAGCCCCTCCCCCGCCCCGGGGCCCC
  GCCCTTCCAGGCGCGGGCTCCGGCCTCCCTGCCCCGGACCCTTGTGACTATGAAGGCCGG
  TTTTCCCGGCTGCATGGTCGTCCCCCGGGGTTCTTGCATTGCGCTTCCTTCGGGGACCCC
  CATGTGCGCAGCTTCCACCATCACTTTCACACATGCCGTGTCCAAGGAGCTTGGCCTCTA
  CTGGATAATGACTTCCTCTTTGTCCAAGCCACCAGCTCCCCCATGGCGTTGGGGGCCAAC
  GCTACCGCCACCCGGAAGCTCACCATCATATTTAAGAACATGCAGGAATGCATTGATCAG
  AAGGTGTATCAGGCTGAGGTGGATAATCTTCCTGTAGCCTTTGAAGATGGTTCTATCAAT
  GGAGGTGACCGACCTGGGGGATCCAGTTTGTCGATTCAAACTGCTAACCCTGGGAACCAT
  GTGGAGATCCAAGCTGCCTACATTGGCACAACTATAATCATTCGGCAGACAGCTGGGCAG
  CTCTCCTTCTCCATCAAGGTAGCAGAGGATGTGGCCATGGCCTTCTCAGCTGAACAGGAC
  CTGCAGCTCTGTGTTGGGGGGTGCCCTCCAAGTCAGCGACTCTCTCGATCAGAGCGCAAT
  CGTCGGGGAGCTATAACCATTGATACTGCCAGACGGCTGTGCAAGGAAGGGCTTCCAGTG
  GAAGATGCTTACTTCCATTCCTGTGTCTTTGATGTTTTAATTTCTGGTGATCCCAACTTT
  ACCGTGGCAGCTCAGGCAGCACTGGAGGATGCCCGAGCCTTCCTGCCAGACTTAGAGAAG
  CTGCATCTCTTCCCCTCAGATGCTGGGGTTCCTCTTTCCTCAGCAACCCTCTTAGCTCCA
  CTCCTTTCTGGGCTCTTTGTTCTGTGGCTTTGCATTCAGTAA
  
  Clone variation with respect to NM_145277.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145277 unedited
  CCGGCGTCCAGAATTTGTATACGATCTCACTATAGGCGGCCGCGAATTCGCACCAGACAC
  GGAGGACCCCTGGCTGGACTGACCCACAGATAGGGAANATGGCTGGAGAATTGGATAGCA
  GAGTAATGTTTGACCTCTGGAAACACTCATTCTCAATGCAAGATCCTCCGCTGCAATGCT
  GAGTACGTATCGTCCACTCTGAGCCTTAGAGGTGGGGGTTCATCAGGAGCACTTCGAGGA
  GGAGGAGGAGGAGGCCGGGGTGGAGGGGTGGGCTCTGGCGGCCTCTGTCGAGCCCTCCGC
  TCCTATGCGCTCTGCACTCGGCGCACCGCCCGCACCTGCCGCGGGGACCTCGCCTTCCAT
  TCGGCGGTACATGGCATCGAAGACCTGATGATCCAGCACAACTGCTCCCGCCAGGGCCCT
  ACAGCCCCTCCCCCGCCCCGGGGCCCCGCCCTTCCAGGCGCGGGCTCCGGCCTCCCTGCC
  CCGGACCCTTGTGACTATGAAGGCCGGTTTTCCCGGCTGCATGGTCGTCCCCCGGGGTTC
  TTGCATTGCGCTTCCTTCGGGGACCCCCATGTGCGCAGCTTCCACCATCACTTTCACACA
  TGCCGTGTCCAAGGAGCTTGGCCTCTACTGGATAATGACTTCCTCTTTGTCCAAGCCACC
  AGCTCCCCCATGGCGTTGGGGGCCAACGCTACCGCCACCCGGAAGCTCACCATCATATTT
  AAGAACATGCAGGAATGCATTGATCAGAAAGTGTATCAGGCCTGAGTGGATAATCTTCCT
  GTAGCCTTTGAAATGGTTCTATCAATGGAGGTGACCGACCTGGGGGNATCCAGTTTGTCG
  ATTCAAACTGCTAAACCCTGGAACCATGTTGAGATCCAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145277 unedited
  ACGACATCGAGNNACCGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTGGTGT
  GAAGAATTTGTAGCTTTAATAACAGATTAGTTAGGGGGTAGGGATGATACTTCTGTCCTA
  CCTGTGCCATTCTTCAAAAGCAGAGACTCCCTTCACCTAAGAGCTAGACAATGCCTGCTT
  CATTTTTGAATCAAGAAAGCAGAACATACCTTACACACTCATATTCATCAAATAGACTTC
  TTTCCCTAATTATGTCTTCCTCCTTTGAGTTTCTTATGGCTAATGATCATGTCTTCTGCT
  TTCAGCTCTTGCCTCTTAAAAATCTCAACCCTTAAATCTTTACTGATCATTAAAACCTTC
  ATGACCTCTAAACTTATAAGGGTGAAATCTGCAGTAAATGGATTATAACAATTTGTTTAC
  TATAAATGAGGCTGGAAAAATTGGTGAAGAGCCCCACAGAGATCCGGAATGCAGTAACCT
  TGCCCAGAATCCTTATTCTGAGATAATTTCAACCCTGGACTGCAGCCTCATCTGACCCTG
  GAAATGTCATTGTTTCACGTGTCTCCTATGCCCTGCATCCTAAAAGAAATCTTTAAATCC
  CTCTATGCCAATCTGTGTCTACAAAACATTCTTCAAACATGAATGGGGACTGAAGGCCCC
  CTTAACAGAAGGGCAAGACCAGAAACAAAGAAGCCCAAAAAACTAGGGCAGCCTATATAC
  GGTTTACTGTGTAGAAAATTAATCAATATGTTATAAAATGAAATAACAATATCCTCCCTC
  TTATTGTTGGTAGGAAAGGCTCCGAGCCATATAATAAATTATTGTACTTAGTCTTGCACG
  GTGATAGAATAATGTGACGACCCGACAATATAAATACATCTAAAATGTCACCCAAGCGAG
  ACATGTTAACTTACGACACTAATAGAAAGAACCTATCGTTACGAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145277
• ORF Size: 942 bp
• Insert Size: 2050
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_145277.3, NP_660320.3
• RefSeq Size: 1961
• RefSeq ORF: 942
• Locus ID: 148738
• Protein Families: Transmembrane
• Gene Summary: The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Two uORFs in the 5' UTR negatively regulate the expression and activity of the encoded protein. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (b) lacks a segment in the 5' UTR and an in-frame portion of the 5' coding region, compared to variant a. The resulting isoform (b) has a shorter N-terminus when compared to isoform a.