ERK5 (MAPK7) (NM_139034) Human Untagged Clone

CAT#: SC124923

MAPK7 (untagged)-Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPK7
• Synonyms: BMK1; ERK4; ERK5; PRKM7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139034, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGAGCCTCTGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGAGCCCCCCGGGCCCGTGAAGGCCG
  AACCCGCCCACACCGCTGCCTCTGTAGCGGCCAAGAACCTGGCCCTGCTTAAAGCCCGCTCCTTCGATGT
  GACCTTTGACGTGGGCGACGAGTACGAGATCATCGAGACCATAGGCAACGGGGCCTATGGAGTGGTGTCC
  TCCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGGCCATCAAGAAGATCCCTAATGCTTTCGATGTGGTGA
  CCAATGCCAAGCGGACCCTCAGGGAGCTGAAGATCCTCAAGCACTTTAAACACGACAACATCATCGCCAT
  CAAGGACATCCTGAGGCCCACCGTGCCCTATGGCGAATTCAAATCTGTCTACGTGGTCCTGGACCTGATG
  GAAAGCGACCTGCACCAGATCATCCACTCCTCACAGCCCCTCACACTGGAACACGTGCGCTACTTCCTGT
  ACCAACTGCTGCGGGGCCTGAAGTACATGCACTCGGCTCAGGTCATCCACCGTGACCTGAAGCCCTCCAA
  CCTATTGGTGAATGAGAACTGTGAGCTCAAGATTGGTGACTTTGGTATGGCTCGTGGCCTGTGCACCTCG
  CCCGCTGAACATCAGTACTTCATGACTGAGTATGTGGCCACGCGCTGGTACCGTGCGCCCGAGCTCATGC
  TCTCTTTGCATGAGTATACACAGGCTATTGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGC
  CCGGCGCCAGCTCTTCCCAGGCAAAAACTATGTACACCAGCTACAGCTCATCATGATGGTGCTGGGTACC
  CCATCACCAGCCGTGATTCAGGCTGTGGGGGCTGAGAGGGTGCGGGCCTATATCCAGAGCTTGCCACCAC
  GCCAGCCTGTGCCCTGGGAGACAGTGTACCCAGGTGCCGACCGCCAGGCCCTATCACTGCTGGGTCGCAT
  GCTGCGTTTTGAGCCCAGCGCTCGCATCTCAGCAGCTGCTGCCCTTCGCCACCCTTTCCTGGCCAAGTAC
  CATGATCCTGATGATGAGCCTGACTGTGCCCCGCCCTTTGACTTTGCCTTTGACCGCGAAGCCCTCACTC
  GGGAGCGCATTAAGGAGGCCATTGTGGCTGAAATTGAGGACTTCCATGCAAGGCGTGAGGGCATCCGCCA
  ACAGATCCGCTTCCAGCCTTCTCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCCAGATGTTGAAATGCCC
  AGTCCCTGGGCTCCCAGTGGGGACTGTGCCATGGAGTCTCCACCACCAGCCCCGCCACCATGCCCCGGCC
  CTGCACCTGACACCATTGATCTGACCCTGCAGCCACCTCCACCAGTCAGTGAGCCTGCCCCACCAAAGAA
  AGATGGTGCCATCTCAGACAATACTAAGGCTGCCCTTAAAGCTGCCCTGCTCAAGTCTTTGAGGAGCCGG
  CTCAGAGATGGCCCCAGCGCACCCCTGGAGGCTCCTGAGCCTCGGAAGCCGGTGACAGCCCAGGAGCGCC
  AGCGGGAGCGGGAGGAGAAGCGGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGGAGAAACGGCGGCAGGA
  GCGGGAGCGAAAGGAACGGGGGGCTGGGGCCTCTGGGGGCCCCTCCACTGACCCCTTGGCTGGACTAGTG
  CTCAGTGACAATGACAGAAGCCTGTTGGAACGCTGGACTCGAATGGCCCGGCCCGCAGCCCCAGCCCTCA
  CCTCTGTGCCGGCCCCTGCCCCAGCGCCAACGCCAACCCCAACCCCAGTCCAACCTACCAGTCCTCCTCC
  TGGCCCTGTAGCCCAGCCCACTGGCCCGCAACCACAATCTGCGGGCTCTACCTCTGGCCCTGTACCCCAG
  CCTGCCTGCCCACCCCCTGGCCCTGCACCCCACCCCACTGGCCCTCCTGGGCCCATCCCTGTCCCCGCGC
  CACCCCAGATTGCCACCTCCACCAGCCTCCTGGCTGCCCAGTCACTTGTGCCACCCCCTGGGCTGCCTGG
  CTCCAGCACCCCAGGAGTTTTGCCTTACTTCCCACCTGGCCTGCCGCCCCCAGACGCCGGGGGAGCCCCT
  CAGTCTTCCATGTCAGAGTCACCTGATGTCAACCTTGTGACCCAGCAGCTATCTAAGTCACAGGTGGAGG
  ACCCCCTGCCCCCTGTGTTCTCAGGCACACCAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCT
  GGAGGAATTCTTAAACCAGTCTTTCGACATGGGCGTGGCTGATGGGCCACAGGATGGCCAGGCAGATTCA
  GCCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGCTCGAAGGCCATGGCATGAACCCTGCCGATATTGAGT
  CCCTGCAGCGTGAGATCCAGATGGACTCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGACCCCTG
  A
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139034 unedited
  TATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGAAGACGCGGAGGTGGTGGCT
  GCGGCCTTTGAACAAGTAAGTGAGCCACCCTCGGAGACCCCCGCGCTGGGGACGGGAGGC
  CGGCGAGCCTCGGGACCTCTGAAAGCCTTGAGGAGGCGCGGGGACACCATGGCCGAGCCT
  CTGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGAGCCCCCCGGGCCCGTGAAGGCCGAA
  CCCGCCCACACCGCTGCCTCTGTAGCGGCCAAGAACCTGGCCCTGCTTAAAGCCCGCTCC
  TTCGATGTGACCTTTGACGTGGGCGACGAGTACGAGATCATCGAGACCATAGGCAACGGG
  GCCTATGGAGTGGTGTCCTCCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGGCCATCAAG
  AAGATCCCTAATGCTTTCGATGTGGTGACCAATGCCAAGCGGACCCTCAGGGAGCTGAAG
  ATCCTCAAGCACTTTAAACACGACAACATCATCGCCATCAAGGACATCCTGAGGCCCACC
  GTGCCCTATGGCGAATTCAAATCTGTCTACGTGGTCCTGGACCTGATGGAAAGCGACCTG
  CACCAGATCATCCACTCCTCACAGCCCCTCCACTGGAACACGTGCGCTACTTCCTGTACC
  AACTGCTGGGCGGGCCTGGAGTACATGCACTCGGCTCAGGTCATCCACCGTGACCTGGAG
  CCCTNCNNACCTATGGTGAATGAGAACTGTGAGCTCAAGATGGNTNGACTTGGTATGGGC
  TCGTGGCCTGGTGCACCTNGCCCGCTGAACATCAGTACCNCATGACTGGAGTATTGTGGC
  CCGCGCCTGGGACCGGGCGGCCCGAGCTCAGCCTCTCTTTGAATGAGATACCCAGCTATT
  GACCCCGGGGTCGGGGGCGGGTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_139034
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139034.1, NP_620603.1
• RefSeq Size: 2813 bp
• RefSeq ORF: 2451 bp
• Locus ID: 5598
• Cytogenetics: 17p11.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (4) is also known as BMK1 beta. It differs in the 5' UTR, as compared to variant 1. Variants 1, 3 and 4 encode the same isoform.