MRPL43 (NM_176794) Human Untagged Clone

CAT#: SC124959

MRPL43 (untagged)-Human mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL43
• Synonyms: bMRP36a; L43mt; MRP-L43
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_176794, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACGGCGCGCGGGACTCCGAGCCGCTTCTTGGCCAGCGTTCTCCACAACGGACTGGGTCGCTATGTGC
  AGCAGCTGCAGCGTCTGAGCTTCAGCGTCAGCCGCGACGGCGCCTCGTCTCGCGGCGCCAGGGAGTTCGT
  GGAGCGGGAGGTGATCGACTTCGCCCGACGGAATCCAGGGGTCGTAATATATGTAAACTCGCGTCCGTGC
  TGCGTGCCCAGAGTAGTGGCCGAATACCTTAACGGGGCTGTGCGCGAGGAGAGCATCCACTGCAAGTCGG
  TCGAGGAGATCTCGACGCTGGTGCAGAAGCTGGCCGACCAGTCGGGCTTGGACGTGATCCGCATCCGCAA
  GCCCTTCCACACCGACAACCCTAGCATCCAGGGCCAGTGGCACCCCTTCACCAACAAGCCGACCACGTTC
  CGCGGGCTACGCCCCCGAGAGGTTCAGGATCCTGCCCCAGCCCAGGATGCGGCTGAGTTCCTCGGCGAAG
  GAGCAGGGCCCTGCTGGTACTCCATTGTTGCTCTGCCTCAGAAGCACATAGCTATTGCCATTCATCCTCC
  GCAGCCGGCTGGGCAGTGCCACCAAGCCATTGGACACTGGCCTGAGACTGTCTGCAGTTGCACCGCAGAT
  CCTCCTGCCCGGCTGGCCCGACCCAATATCAGTTCAGTCATCCGATCTTCCTTGGGGAAATACCCATTAC
  CGTCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_176794 unedited
  GTTTTGTATACGACTCACTATAGGCGGCCGCGACATTCGCACGAGGTCCCGCCCCCCTCT
  CCTCGCTGCTTAGGCTCCGCGGCCTCCAAGCTGTAGCTATGACGGCGCGCGGGACTCCGA
  GCCGCTTCTTGGCCAGCGTTCTCCACAACGGACTGGGTCGCTATGTGCAGCAGCTGCAGC
  GTCTGAGCTTCAGCGTCAGCCGCGACGGCGCCTCGTCTCGCGGCGCCAGGGAGTTCGTGG
  AGCGGGAGGTGATCGACTTCGCCCGACGGAATCCAGGGGTCGTAATATATGTAAACTCGC
  GTCCGTGCTGCGTGCCCAGAGTAGTGGCCGAATACCTTAACGGGGCTGTGCGCGAGGAGA
  GCATCCACTGCAAGTCGGTCGAGGAGATCTCGACGCTGGTGCAGAAGCTGGCCGACCAGT
  CGGGCTTGGACGTGATCCGCATCCGCAAGCCCTTCCACACCGACAACCCTAGCATCCAGG
  GCCAGTGGCACCCCTTCACCAACAAGCCGACCACGNNNCCCGCGGGCTACCCCCGGAAAG
  GGGTCCAGGATCCTGCCCCAGCCCAGGACACTGGCCTGAGACTGGCTGCAGTTGCACCGC
  AGATCCTCCTGCCCCGGCTGGCCCGACCAATATCAANTTCAGTCATCCGATCTTTCTTTG
  GGNGAAATACCCATTACCGTCCTGAACCCTTGTCATCCACTACTTGCCTATAGCTNCCAG
  AAGTGACAAGACACTTCAATCTNAAAATATGTTTCCTAGTNTATATTCATTGGGCAGTAA
  AGGGGTTGTTGAGCACTTAAAAAAAAAAAAAAACTCACTCTANATGGGGCCGCGTCATTA
  CTGTTTCTGACAAATCCCCGGGGGGGAATCCCTGGGACCCTTCCCAGGCCTTCTGGCCCC
  GGGGAAGTTGCCCTCCATGCCCACAGGCTTGCTATAAAAATTAGTGATCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_176794
• ORF Size: 708 bp
• Insert Size: 700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_176794.1, NP_789764.1
• RefSeq Size: 894
• RefSeq ORF: 708
• Locus ID: 84545
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (4) differs in the 3' UTR and has multiple coding region differences, compared to variant 1. This results in a longer isoform (d) with a distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.