TCF21 (NM_198392) Human Untagged Clone

CAT#: SC125048

TCF21 (untagged)-Human transcription factor 21 (TCF21), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCF21
• Synonyms: bHLHa23; POD1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_198392, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCACCGGCTCCCTCAGCGATGTGGAGGACCTTCAAGAGGTGGAGATGTTGGAATGTGACGGGTTGA
  AAATGGATTCGAACAAGGAATTTGTGACTTCCAACGAGAGCACCGAGGAGAGCTCCAACTGCGAGAATGG
  GTCTCCCCAGAAGGGCCGCGGCGGCCTGGGCAAGAGGAGGAAGGCGCCCACCAAGAAGAGCCCCCTGAGC
  GGGGTCAGCCAGGAGGGGAAGCAGGTCCAGCGCAACGCCGCCAACGCGCGAGAGCGGGCCCGCATGCGAG
  TGCTGAGCAAGGCCTTCTCCAGACTCAAGACCACCCTGCCCTGGGTGCCCCCCGACACCAAGCTCTCCAA
  GCTGGACACGCTCAGGCTGGCGTCCAGCTACATCGCCCACTTGAGGCAGATCCTGGCTAACGACAAATAC
  GAGAACGGGTACATTCACCCGGTCAACCTGACGTGGCCCTTTATGGTGGCCGGGAAACCCGAGAGTGACC
  TGAAAGAAGTGGTGACCGCGAGCCGCTTATGTGGAACCACCGCGTCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198392 unedited
  CCCCCCCGCCCCCCCCCCTTTTCTCCCCCCCCCCGGTTCAGNATTTGTNATACGACTCAC
  TATAGGCGGCCGCGNAATTCGCACCAGCCACGACTCTGGGAGTGGGGAAACAGAGAGCCG
  GTTCCTCTGCTGCAGAAGTCCTCGGGGTTCCTTCTCACAACTCTGCGAAGGGGAAAGGGT
  TGTGAGACCCAACCAGACCCCAACTCCAGCTCCCAGCAGGAGGTGGCTGCGCCACACTCG
  GGAGGCCTCTTGGTTTCAGGGTCTCTCTGTCTCTCTCTCACCCTCTTCCTCGCTTTCTCT
  GTCTCTCTGTCTCTCTCTCTCTCTCTCCCTCGTCCACTCCCCCAAACATGTCCACCGGCT
  CCCTCAGCGATGTGGAGGACCTTCAAGAGGTGGAGATGTTGGAATGTGACGGGTTGAAAA
  TGGATTCGAACAAGGAATTTGTGACTTCCAACGAGAGCACCGAGGAGAGCTCCAACTGCG
  AGAATGGGTCTCCCCAGAAGGGCCGCGGCGGCCTGGGCAAGAGGAGGAAGGCGCCCACCA
  AGAAGAGCCCCCTGAGCGGNGTCAGCCAGGAGGGGAAGCAGGTCCAGCGCAACGCCGCCA
  ACGCGCGAGAGCGGGCCCGCATGCGAGTGCTGAGCAAGGCCTTCTCCAGACTCAAGACCA
  CCCTGCCCTGGGTGCCCCCCGACACCAAGCTCTCCAAGCTGGACACGCTCCAGCTGGCGT
  TCAGCTACATCGNCCACTTGAGGCAGATCCTGGCTAACGACAAATACGAGAACCGGTACC
  ATTCACCCGGTCACCCTGACGTGGCCCTTTATGGTGGCCCGGGAAACCCCGAGAGTGACC
  TGAANGAAAATGTGACCGCCAGCCGCTNATGTGGAACCCACCGCGTCCTGACTTTGNAGG
  TGCCAGTCTTGGAAAAGCCCCGCTCCCGGGGGGAGACCGCCCCCGGAAGGCGACCCCTGG
  CCTCAGGGCTCTCTTTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198392
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198392.1, NP_938206.1
• RefSeq Size: 3231 bp
• RefSeq ORF: 540 bp
• Locus ID: 6943
• Cytogenetics: 6q23.2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'TCF21 encodes a transcription factor of the basic helix-loop-helix family. The TCF21 product is mesoderm specific, and expressed in embryonic epicardium, mesenchyme-derived tissues of lung, gut, gonad, and both mesenchymal and glomerular epithelial cells in the kidney. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.