IL2 (NM_000586) Human Untagged Clone

CAT#: SC125234

IL2 (untagged)-Human interleukin 2 (IL2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL2
• Synonyms: IL-2; lymphokine; TCGF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125234 sequence for NM_000586 edited (data generated by NextGen Sequencing)
  ATGTACAGGATGCAACTCCTGTCTTGCATTGCACTAAGTCTTGCACTTGTCACAAACAGT
  GCACCTACTTCAAGTTCTACAAAGAAAACACAGCTACAACTGGAGCATTTACTTCTGGAT
  TTACAGATGATTTTGAATGGAATTAATAATTACAAGAATCCCAAACTCACCAGGATGCTC
  ACATTTAAGTTTTACATGCCCAAGAAGGCCACAGAACTGAAACATCTTCAGTGTCTAGAA
  GAAGAACTCAAACCTCTGGAGGAAGTGCTAAATTTAGCTCAAAGCAAAAACTTTCACTTA
  AGACCCAGGGACTTAATCAGCAATATCAACGTAATAGTTCTGGAACTAAAGGGATCTGAA
  ACAACATTCATGTGTGAATATGCTGATGAGACAGCAACCATTGTAGAATTTCTGAACAGA
  TGGATTACCTTTTGTCAAAGCATCATCTCAACACTGACTTGA
  
  Clone variation with respect to NM_000586.3
  114 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000586 unedited
  GACTCACTATAGGGCGGCCGCGAATTCCCTGCCACAATGTACAGGATGCAACTCCTGTCT
  TGCATTGCACTAAGTCTTGCACTTGTCACAAACAGTGCACCTACTTCAAGTTCTACAAAG
  AAAACACAGCTACAACTGGAGCATTTACTTCTGGATTTACAGATGATTTTGAATGGAATT
  AATAATTACAAGAATCCCAAACTCACCAGGATGCTCACATTTAAGTTTTACATGCCCAAG
  AAGGCCACAGAACTGAAACATCTTCAGTGTCTAGAAGAAGAACTCAAACCTCTGGAGGAA
  GTGCTAAATTTAGCTCAAAGCAAAAACTTTCACTTAAGACCCAGGGACTTAATCAGCAAT
  ATCAACGTAATAGTTCTGGAACTAAAGGGATCTGAAACAACATTCATGTGTGAATATGCT
  GATGAGACAGCAACCATTGTAGAATTTCTGAACAGATGGATTACCTTTTGTCAAAGCATC
  ATCTCAACACTGACTTGATAATTAAGTGCTTCCCACTTAAAACATATCAGGCCTCGACTC
  TAGATTGCGGCCGCGGTCATANGCTGTTCCTGAACAGATTCCGGGTGGGATCCTTGTGAC
  CCTTCCCATGGCGCTTTCGGGCCCGGAGTAGTCCTACTCAGGGCACACAACTTGGCCATA
  AAAATAAATGGCACAATTTGTCCGCGAGAGGGGCCTCTAAAATATAGGGGGGGGGGGGGG
  GGGGGGGGGGGCGCTTGGAAACACCGGTGGGCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000586 unedited
  ACCCATGGATCCTGGGCTGATGGCACTTNCCAGGNCCAGNAAAGCACTGGGGNAGGGTCA
  CAGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAG
  TCGAGGCCTGATATGTTTTAAGTGGGAAGCACTTAATTATCAAGTCAGTGTTGAGATGAT
  GCTTTGACAAAAGGTAATCCATCTGTTCAGAAATTCTACAATGGTTGCTGTCTCATCAGC
  ATATTCACACATGAATGTTGTTTCAGATCCCTTTAGTTCCAGAACTATTACGTTGATATT
  GCTGATTAAGTCCCTGGGTCTTAAGTGAAAGTTTTTGCTTTGAGCTAAATTTAGCACTTC
  CTCCAGAGGTTTGAGTTCTTCTTCTAGACACTGAAGATGTTTCAGTTCTGTGGCCTTCTT
  GGGCATGTAAAACTTAAATGTGAGCATCCTGGTGAGTTTGGGATTCTTGTAATTATTAAT
  TCCATTCAAAATCATCTGTAAATCCAGAAGTAAATGCTCCAGTTGTAGCTGTGTTTTCTT
  TGTAGAACTTGAAGTAGGTGCACTGTTTGTGACAAGTGCAAGACTTAGTGCAATGCAAGA
  CAGGAGTTGCATCCTGTACATTGTGGCAGGGAATTCGCGGCCGCCCTATAGTGAGTCGTA
  TTACANAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACAC
  GCCTACCGCCCATTTGCGTCAACGGNGCGGGGTTATTACGACATTTTGGAAAGTCCNGTT
  GATTTTGGGTGCAAAACANACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCC
  GTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGGGCAAACCGCATCACCATGGTAA
  TAGCGATGACTATACGTAGATTACTGGCAT
  
• Restriction Sites: Please inquire
• ACCN: NM_000586
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000586.2, NP_000577.2
• RefSeq Size: 1047 bp
• RefSeq ORF: 462 bp
• Locus ID: 3558
• Cytogenetics: 4q27
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus
• Gene Summary: 'This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Sep 2020]'