TNF alpha (TNF) (NM_000594) Human Untagged Clone

CAT#: SC125235

TNF (untagged)-Human tumor necrosis factor (TNF)


  "NM_000594" in other vectors (6)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TNF
Synonyms DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC125235 sequence for NM_000594 edited (data generated by NextGen Sequencing)
ATGAGCACTGAAAGCATGATCCGGGACGTGGAGCTGGCCGAGGAGGCGCTCCCCAAGAAG
ACAGGGGGGCCCCAGGGCTCCAGGCGGTGCTTGTTCCTCAGCCTCTTCTCCTTCCTGATC
GTGGCAGGCGCCACCACGCTCTTCTGCCTGCTGCACTTTGGAGTGATCGGCCCCCAGAGG
GAAGAGTTCCCCAGGGACCTCTCTCTAATCAGCCCTCTGGCCCAGGCAGTCAGATCATCT
TCTCGAACCCCGAGTGACAAGCCTGTAGCCCATGTTGTAGCAAACCCTCAAGCTGAGGGG
CAGCTCCAGTGGCTGAACCGCCGGGCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGA
GATAACCAGCTGGTGGTGCCATCAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTC
AAGGGCCAAGGCTGCCCCTCCACCCATGTGCTCCTCACCCACACCATCAGCCGCATCGCC
GTCTCCTACCAGACCAAGGTCAACCTCCTCTCTGCCATCAAGAGCCCCTGCCAGAGGGAG
ACCCCAGAGGGGGCTGAGGCCAAGCCCTGGTATGAGCCCATCTATCTGGGAGGGGTCTTC
CAGCTGGAGAAGGGTGACCGACTCAGCGCTGAGATCAATCGGCCCGACTATCTCGACTTT
GCCGAGTCTGGGCAGGTCTACTTTGGGATCATTGCCCTGTGA

Clone variation with respect to NM_000594.2
>OriGene 5' read for NM_000594 unedited
GACTCACTATAGGGCGGCCGCGAATTCGAAAGGACACCATGAGCACTGAAAGCATGATCC
GGGACGTGGAGCTGGCCGAGGAGGCGCTCCCCAAGAAGACAGGGGGGCCCCAGGGCTCCA
GGCGGTGCTTGTTCCTCAGCCTCTTCTCCTTCCTGATCGTGGCAGGCGCCACCACGCTCT
TCTGCCTGCTGCACTTTGGAGTGATCGGCCCCCAGAGGGAAGAGTTCCCCAGGGACCTCT
CTCTAATCAGCCCTCTGGCCCAGGCAGTCAGATCATCTTCTCGAACCCCGAGTGACAAGC
CTGTAGCCCATGTTGTAGCAAACCCTCAAGCTGAGGGGCAGCTCCAGTGGCTGAACCGCC
GGGCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGAGATAACCAGCTGGTGGTGCCAT
CAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTCAAGGGCCAAGGCTGCCCCTCCA
CCCATGTGCTCCTCACCCACACCATCAGCCGCATCGCCGTCTCCTACCAGACCAAGGTCA
ACCTCCTCTCTGCCATCAAGAGCCCCTGCCAGAGGGAGACCCCAGAGGGGCTGAGGCCAG
CCCTGTATGAGCCCATCTTCTTGAAGGGTCTTCGCTTGAGAGGTGACCGATTAACCTGAA
TCAATGGCCGAATATCTGACTTTCCAGCTTGGCAGGTTAATTGGATCATTGCTGGAAGCC
CCCTTATTGGGCCGGGCATATTTT
>OriGene 3' read for NM_000594 unedited
GCGAGCACTGGGGNAGTGGGTCACAGGGCATGCCACCCGGGTATCTGTTGCAGGAAAACA
GCTATGACCGCGGCCGCAATCTAGAGTCGAGCCTCACAGGGCAATGATCCCAAAGTAGAC
CTGCCCAGACTCGGCAAAGTCGAGATAGTCGGGCCGATTGATCTCAGCGCTGAGTCGGTC
ACCCTTCTCCAGCTGGAAGACCCCTCCCAGATAGATGGGCTCATACCAGGGCTTGGCCTC
AGCCCCCTCTGGGGTCTCCCTCTGGCAGGGGCTCTTGATGGCAGAGAGGAGGTTGACCTT
GGTCTGGTAGGAGACGGCGATGCGGCTGATGGTGTGGGTGAGGAGCACATGGGTGGAGGG
GCAGCCTTGGCCCTTGAAGAGGACCTGGGAGTAGATGAGGTACAGGCCCTCTGATGGCAC
CACCAGCTGGTTATCTCTCAGCTCCACGCCATTGGCCAGGAGGGCATTGGCCCGGCGGTT
CAGCCACTGGAGCTGCCCCTCAGCTTGAGGGTTTGCTACAACATGGGCTACAGGCTTGTC
ACTCGGNGTTCGAGAAGATGATCTGACTGCCTGGGCCAGAGGGCTGATTAGAGAGAGGTC
CCTGGGGAACTCTTCCCTCTGGGGGCCGATCACTCCANAGTGCAGCAGGCAGAAGAGCGT
GGGTGGCGCCTGCCACGATCAGGGAAGAGAAGAGGCTGAGGAAACAGCACCGCCTGGAGC
CCTGGGGCCCCCCTGTCTTCTTGGGGGAGCGCCTCTCGCCAGCTCCACGTCCCGGGATCA
TGCTTNCAGTGCTCATGGTGGTCCTTTCGATTTCGGGGCGCCCTATGNTGAGTCGTATTA
CAAAATTCTGACGGTTCACTAAACGAGCTTTGCTTATTTAGACCTCCCACCGTACACGC
Restriction Sites Please inquire     
ACCN NM_000594
Insert Size 700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000594.2, NP_000585.2
RefSeq Size 1669 bp
RefSeq ORF 702 bp
Locus ID 7124
Cytogenetics 6p21.33
Protein Families Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus
Gene Summary 'This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]'

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