IL6 (NM_000600) Human Untagged Clone

CAT#: SC125236

IL6 (untagged)-Human interleukin 6 (interferon, beta 2) (IL6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL6
• Synonyms: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125236 sequence for NM_000600 edited (data generated by NextGen Sequencing)
  ATGAACTCCTTCTCCACAAGCGCCTTCGGTCCAGTTGCCTTCTCCCTGGGGCTGCTCCTG
  GTGTTGCCTGCTGCCTTCCCTGCCCCAGTACCCCCAGGAGAAGATTCCAAAGATGTAGCC
  GCCCCACACAGACAGCCACTCACCTCTTCAGAACGAATTGACAAACAAATTCGGTACATC
  CTCGACGGCATCTCAGCCCTGAGAAAGGAGACATGTAACAAGAGTAACATGTGTGAAAGC
  AGCAAAGAGGCACTGGCAGAAAACAACCTGAACCTTCCAAAGATGGCTGAAAAAGATGGA
  TGCTTCCAATCTGGATTCAATGAGGAGACTTGCCTGGTGAAAATCATCACTGGTCTTTTG
  GAGTTTGAGGTATACCTAGAGTACCTCCAGAACAGATTTGAGAGTAGTGAGGAACAAGCC
  AGAGCTGTGCAGATGAGTACAAAAGTCCTGATCCAGTTCCTGCAGAAAAAGGCAAAGAAT
  CTAGATGCAATAACCACCCCTGACCCAACCACAAATGCCAGCCTGCTGACGAAGCTGCAG
  GCACAGAACCAGTGGCTGCAGGACATGACAACTCATCTCATTCTGCGCAGCTTTAAGGAG
  TTCCTGCAGTCCAGCCTGAGGGCTCTTCGGCAAATGTAG
  
  Clone variation with respect to NM_000600.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000600 unedited
  GACTCACTATAGGGCGGCCGCGAATTCAGCCCAGCTATGAACTCCTTCTCCACAAGCGCC
  TTCGGTCCAGTTGCCTTCTCCCTGGGGCTGCTCCTGGTGTTGCCTGCTGCCTTCCCTGCC
  CCAGTACCCCCAGGAGAAGATTCCAAAGATGTAGCCGCCCCACACAGACAGCCACTCACC
  TCTTCAGAACGAATTGACAAACAAATTCGGTACATCCTCGACGGCATCTCAGCCCTGAGA
  AAGGAGACATGTAACAAGAGTAACATGTGTGAAAGCAGCAAAGAGGCACTGGCAGAAAAC
  AACCTGAACCTTCCAAAGATGGCTGAAAAAGATGGATGCTTCCAATCTGGATTCAATGAG
  GAGACTTGCCTGGTGAAAATCATCACTGGTCTTTTGGAGTTTGAGGTATACCTAGAGTAC
  CTCCAGAACAGATTTGAGAGTAGTGAGGAACAAGCCAGAGCTGTGCAGATGAGTACAAAA
  GTCCTGATCCAGTTCCTGCAGAAAAAGGCAAAGAATCTAGATGCAATAACCACCCCTGAC
  CCAACCACAAATGCCAGCCTGCTGACGAAGCTGCAGGCACAGAACCAGTGGCTGCAGGAC
  TTGACAACTTATCTCATTCTGCGCAGCTTTAGGAGTTCCGGAGTTCACCTGAGGGCTTTC
  GGAAAAGTACATGGGCACTCATTCTTGATTTTGTTGGGGGCGCGGAATATTTTTCCTAAA
  AATCCGGGGGGCTCTGGGACCCTCCCAGGGTTTTGGGCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000600 unedited
  NCCCCTCCATTGCGCAATTGGCGAAGGCCAATTTCCAGGGCCAGNACGAGCACTGGGGNA
  GGGTTCACAGGGCATGCCCCCGGGCACTGTTCAGGAAACAGCTATGACCGCGGCCGCAAT
  CTAGAGTCGAGATCTGAGGTGCCCATGCTTACATTTGCCGAAGAGCCCTCAGGCTGGACT
  GCAGGAACTCCTTAAAGCTGCGCAGAATGAGATGAGTTGTCATGTCCTGCAGCCACTGGT
  TCTGTGCCTGCAGCTTCGTCAGCAGGCTGGCATTTGTGGTTGGGTCAGGGGTGGTTATTG
  CATCTAGATTCTTTGCCTTTTTCTGCAGGAACTGGATCAGGACTTTTGTACTCATCTGCA
  CAGCTCTGGCTTGTTCCTCACTACTCTCAAATCTGTTCTGGAGGTACTCTAGGTATACCT
  CANACTCCAAAAGACCAGTGATGATTTTCACCAGGCAAGTCTCCTCATTGAATCCAGATT
  GGAAGCATCCATCTTTTTCAGCCATCTTTGGAAGGTTCAGGTTGTTTTCTGCCAGTGCCT
  CTTTGCTGCTTTCACACATGTTACTCTTGTTACATGTCTCCTTTCTCAGGGCTGAGATGC
  CGTCGAGGATGTACCGAATTTGTTTGTCAATTCGTTCTGAAGAGGTGAGTGGCTGTCTGT
  GTGGNGCGGCTACATCTTTGGAATCTTCTCCTGGGGGTACTGGGGCAGGGAAGGCAGCAG
  GCAACACCAGNAGCAGCCCCAGGGGAGAAGCAACTGGACCCGAAGCGCTTGTGGAGAANG
  AGTTCATAGCTGGGCTGAATTCGCCGCCGCCTATAGTGAGTCGTATACAAAATTCTGACG
  TTCACTAACGAGCTTTGCTATTTAGACCTCCNACGTACACGCCTACCGCCAT
  
• Restriction Sites: Please inquire
• ACCN: NM_000600
• Insert Size: 800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000600.1, NP_000591.1
• RefSeq Size: 1125 bp
• RefSeq ORF: 639 bp
• Locus ID: 3569
• Cytogenetics: 7p15.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Jak-STAT signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prion diseases, Toll-like receptor signaling pathway
• Gene Summary: 'This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).