GLUT4 (SLC2A4) (NM_001042) Human Untagged Clone

CAT#: SC125240

SLC2A4 (untagged)-Human solute carrier family 2 (facilitated glucose transporter), member 4 (SLC2A4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC2A4
• Synonyms: GLUT4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125240 sequence for NM_001042 edited (data generated by NextGen Sequencing)
  ATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGATGGGGAACCCCCTCAGCAGCGAGTG
  ACTGGGACCCTGGTCCTTGCTGTGTTCTCTGCGGTGCTTGGCTCCCTGCAGTTTGGGTAC
  AACATTGGGGTCATCAATGCCCCTCAGAAGGTGATTGAACAGAGCTACAATGAGACGTGG
  CTGGGGAGGCAGGGGCCTGAGGGACCCAGCTCCATCCCTCCAGGCACCCTCACCACCCTC
  TGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGGCATGATTTCCTCCTTCCTCATTGGT
  ATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCATGCTGGTCAACAATGTCCTGGCGGTG
  CTGGGGGGCAGCCTCATGGGCCTGGCCAATGCTGCTGCCTCCTATGAAATGCTCATCCTT
  GGACGATTCCTCATTGGCGCCTACTCAGGGCTGACATCAGGGCTGGTGCCCATGTACGTG
  GGGGAGATTGCTCCCACTCACCTGCGGGGCGCCCTGGGGACGCTCAACCAACTGGCCATT
  GTTATCGGCATTCTGATCGCCCAGGTGCTGGGCTTGGAGTCCCTCCTGGGCACTGCCAGC
  CTGTGGCCACTGCTCCTGGGCCTCACAGTGCTACCTGCCCTCCTGCAGCTGGTCCTGCTG
  CCCTTCTGTCCCGAGAGCCCCCGCTACCTCTACATCATCCAGAATCTCGAGGGGCCTGCC
  AGAAAGAGTCTGAAGCGCCTGACAGGCTGGGCCGATGTTTCTGGAGTGCTGGCTGAGCTG
  AAGGATGAGAAGCGGAAGCTGGAGCGTGAGCGGCCACTGTCCCTGCTCCAGCTCCTGGGC
  AGCCGTACCCACCGGCAGCCCCTGATCATTGCGGTCGTGCTGCAGCTGAGCCAGCAGCTC
  TCTGGCATCAATGCTGTTTTCTATTATTCGACCAGCATCTTCGAGACAGCAGGGGTAGGC
  CAGCCTGCCTATGCCACCATAGGAGCTGGTGTGGTCAACACAGTCTTCACCTTGGTCTCG
  GTGTTGTTGGTGGAGCGGGCGGGGCGCCGGACGCTCCATCTCCTGGGCCTGGCGGGCATG
  TGTGGCTGTGCCATCCTGATGACTGTGGCTCTGCTCCTGCTGGAGCGAGTTCCAGCCATG
  AGCTACGTCTCCATTGTGGCCATCTTTGGCTTCGTGGCATTTTTTGAGATTGGCCCTGGC
  CCCATTCCTTGGTTCATCGTGGCCGAGCTCTTCAGCCAGGGACCCCGCCCGGCAGCCATG
  GCTGTGGCTGGTTTCTCCAACTGGACGAGCAACTTCATCATTGGCATGGGTTTCCAGTAT
  GTTGCGGAGGCTATGGGGCCCTACGTCTTCCTTCTATTTGCGGTCCTCCTGCTGGGCTTC
  TTCATCTTCACCTTCTTAAGAGTACCTGAAACTCGAGGCCGGACGTTTGACCAGATCTCA
  GCTGCCTTCCACCGGACACCCTCTCTTTTAGAGCAGGAGGTGAAACCCAGCACAGAACTT
  GAGTATTTAGGGCCAGATGAGAACGACTGA
  
  Clone variation with respect to NM_001042.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001042 unedited
  GGGGAAGGAGNNNTTTAGTCGATTTGTATACGAAGGNNTTANGGCGGCCGCGAATTCGGC
  ACGAGACCAGATCCGCGGGAGCCCCACTGCTCTCCGGGTCCTTGGCTTGTGGCTGTGGGT
  CCCATCGGGCCCGCCCTCGCACGTCACTCCGGGACCCCCGCGGCCTCCGCAGGTTCTGCG
  CTCCAGGCCGGAGTCAGAGACTCCAGGATCGGTTCTTTCATCTTCGCCGCCCCTGCGCGT
  CCAGCTCTTCTAAGACGAGATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGATGGGGA
  ACCCCCTCAGCAGCGAGTGACTGGGACCCTGGTCCTTGCTGTGTTCTCTGCGGTGCTTGG
  CTCCCTGCAGTTTGGGTACAACATTGGGGTCATCAATGCCCCTCAGAAGGTGATTGAACA
  GAGCTACAATGAGACGTGGCTGGGGAGGCAGGGGCCTGAGGGACCCAGCTCCATCCCTCC
  AGGCACCCTCACCACCCTCTGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGGCATGAT
  TTCCTCCTTCCTCATTGGTATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCATGCTGGT
  CAACAATGTCCTTGCGGTGCTGGGGGCAGCCTCATGGGCCTGGCCATGCTGCTGCTCTAT
  GAATGCTATCNTGACGATCTCATGCGCTATCGGCTGCTCAGCTGTCCATGTCTGGGAATG
  TCATACTGCGGGCCTGGACTACACTGCTGTTGC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001042 unedited
  TGGCATGGGTTTCAGTATGTTGCGGAGGCTATGGGGCCCTACGTCTTCCTTCTATTTGCG
  GTCCTCCTGCTGGGCTTCTTCATCTTCACCTTCTTAAGAGTACCTGAAACTCGAGGCCGG
  ACGTTTGACCAGATCTCAGCTGCCTTCCACCGGACACCCTCTCTTTTAGAGCAGGAGGTG
  AAACCCAGCACAGAACTTGAGTATTTAGGGCCAGATGAGAACGACTGAGGGGCCAGGCAG
  GGGTGGGAGAGCCAGCTCTCTCTACCCGGCCCAGAGACCCCTTCCTTTCCTCTGCAGCAC
  TTTAACCCTCTCTTCCCTATTATTTCCGGGTGGAAAAGAATCCCTGCAGCCTGGTAGAAT
  TGGGAAGCTGGGGGAAGGGTGGTCTGAGCACCCCCTCATTCCCCTCGTGTGACTCTCTTG
  GATTATTTATGTGTTGTGGTTTGGCCGTGGCCATCAGGGTGGGCCACTCTCCCCTCCCTC
  TTCCTTCCCCCATCCCCTTTCCTCCCCACCTTCCCCAGACTCAGCTCCAGAATACCTTCT
  TCGCTGCTAGAGAAGGGGGATTGGAGGGAAGACAGGTCTAGACTTTCTCAGTGGGACAAA
  CCAGAGCAGAGAGCAGGACAGGAGACAAGAAATCCAGTTTCCCACCACCTTGGACTCCTC
  CCACAATCTGGGACTTTCACTGAATTCTTGCCACGCAGACTCTGGGCAAAGGGGTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTGAGACAGTCTCGCTCTGTCGCCCAGGCTCGAGTGCAG
  TGGCGTGATCTTG
  
• Restriction Sites: Please inquire
• ACCN: NM_001042
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042.1, NP_001033.1
• RefSeq Size: 2128 bp
• RefSeq ORF: 1530 bp
• Locus ID: 6517
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Type II diabetes mellitus
• Gene Summary: 'This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]'