LTB (NM_002341) Human Untagged Clone

CAT#: SC125270

LTB (untagged)-Human lymphotoxin beta (TNF superfamily, member 3) (LTB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LTB
• Synonyms: p33; TNFC; TNFSF3; TNLG1C
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125270 sequence for NM_002341 edited (data generated by NextGen Sequencing)
  ATGGGGGCACTGGGGCTGGAGGGCAGGGGTGGGAGGCTCCAGGGGAGGGGTTCCCTCCTG
  CTAGCTGTGGCAGGAGCCACTTCTCTGGTGACCTTGTTGCTGGCGGTGCCTATCACTGTC
  CTGGCTGTGCTGGCCTTAGTGCCCCAGGATCAGGGAGGACTGGTAACGGAGACGGCCGAC
  CCCGGGGCACAGGCCCAGCAAGGACTGGGGTTTCAGAAGCTGCCAGAGGAGGAGCCAGAA
  ACAGATCTCAGCCCCGGGCTCCCAGCTGCCCACCTCATAGGCGCTCCGCTGAAGGGGCAG
  GGGCTAGGCTGGGAGACGACGAAGGAACAGGCGTTTCTGACGAGCGGGACGCAGTTCTCG
  GACGCCGAGGGGCTGGCGCTCCCGCAGGACGGCCTCTATTACCTCTACTGTCTCGTCGGC
  TACCGGGGCCGGGCGCCCCCTGGCGGCGGGGACCCCCAGGGCCGCTCGGTCACGCTGCGC
  AGCTCTCTGTACCGGGCGGGGGGCGCCTACGGGCCGGGCACTCCCGAGCTGCTGCTCGAG
  GGCGCCGAGACGGTGACTCCAGTGCTGGACCCGGCCAGGAGACAAGGGTACGGGCCTCTC
  TGGTACACGAGCGTGGGGTTCGGCGGCCTGGTGCAGCTCCGGAGGGGCGAGAGGGTGTAC
  GTCAACATCAGTCACCCCGATATGGTGGACTTCGCGAGAGGGAAGACCTTCTTTGGGGCC
  GTGATGGTGGGGTGA
  
  Clone variation with respect to NM_002341.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002341 unedited
  TCACCAATCGTAATACGCGCGACTATATGGCGGCCGCGAATTCGGCACCAGAAAGTCCCA
  GCCAGGTGTGCAGGTCTACAGGGAGGGGGTGGGCCCGTCCCTGAGGTATGAAAGCCCCCT
  GCTCTGGCTCTGGTTCAGTCTCAATGGGGGCACTGGGGCTGGAGGGCAGGGGTGGGAGGC
  TCCAGGGGAGGGGTTCCCTCCTGCTAGCTGTGGCAGGAGCCACTTCTCTGGTGACCTTGT
  TGCTGGCGGTGCCTATCACTGTCCTGGCTGTGCTGGCCTTAGTGCCCCAGGATCAGGGAG
  GACTGGTAACGGAGACGGCCGACCCCGGGGCACAGGCCCAGCAAGGACTGGGGTTTCAGA
  AGCTGCCAGAGGAGGAGCCAGAAACAGATCTCAGCCCCGGGCTCCCAGCTGCCCACCTCA
  TAGGCGCTCCGCTGAAGGGGCAGGGGCTAGGCTGGGAGACGACGAAGGAACAGGCGTTTC
  TGACGAGCGGGACGCAGTTCTCGGACGCCGAGGGGCTGGCGCTCCCGCAGGACGGCCTCT
  ATTACCTCTACTGTCTCGTCGGCTACCGGGGCCGGGCGCCCCCTGGCGGCGGGGACCCCC
  AGGGCCGCTCGGTCACGCTGCGCAGCTCTCTGTACCGGGCGGGGGGCGCCTACGGGCCGG
  GCACTCCCGAGCTGCTGCTCGAGGGCGCCGAGACCGTGACTCCAGTGCTGGGACCCGCCA
  GGAGACAAGGGTACGGGCCTCTCTGTACACGAGCGTGNGGTTCGCGGCCTGGTGCACTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002341 unedited
  GTTGCAACTTCCCGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTTATCGGCAGCACTGAAGCTTTCCATTCTTTATTTTCATCAGGTTCAAAATCAATTTC
  CAAACAGTCTCCTACATTTTTCCCACTGCCATGGGGTCCTGGGCGTCCGGGCCCCCAATA
  TTCACGCACTCGCACCACGCACTCATATTCCCTCACCCCACCATCACGGCCCCAAAGAAG
  GTCTTCCCTCTCGCGAAGTCCACCATATCGGGGTGACTGATGTTGACGTACACCCTCTCG
  CCCCTCCGGAGCTGCACCAGGCCGCCGAACCCCACGCTCGTGTACCAGAGAGGCCCGTAC
  CCTTGTCTCCTGGCCGGGTCCAGCACTGGAGTCACCGTCTCGGCGCCCTCGAGCAGCAGC
  TCGGGAGTGCCCGGCCCGTAGGCGCCCCCCGCCCGGTACAGAGAGCTGCGCAGCGTGACC
  GAGCGNGCCCCTGGGGTCCCCGCCGCCAGGGGGCGCCCGGCCCCGGTAGCCGACNAAGCA
  GTAGAGGTAATAGAGGCCCGTCTGCGGNNAGCGCCAGCCCCTCGCGTCCGANGAACTGCG
  TCCCGCTCGTCAGAAACGCCTGTCCCTCGTCGTCTCCCAGCCTAGCCCCTGCCCCTTCAG
  CGGACGGCCTTATAGTTGGCAACTTGGACCCCGGCCCTAGTAT
  
• Restriction Sites: Please inquire
• ACCN: NM_002341
• Insert Size: 900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002341.1, NP_002332.1
• RefSeq Size: 894 bp
• RefSeq ORF: 735 bp
• Locus ID: 4050
• Cytogenetics: 6p21.33
• Domains: TNF
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction
• Gene Summary: 'Lymphotoxin beta is a type II membrane protein of the TNF family. It anchors lymphotoxin-alpha to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the lymphotoxin-alpha 1/beta 2 complex (e.g. 1 molecule alpha/2 molecules beta) and this complex is the primary ligand for the lymphotoxin-beta receptor. The minor complex is lymphotoxin-alpha 2/beta 1. LTB is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. Lymphotoxin-beta isoform b is unable to complex with lymphotoxin-alpha suggesting a function for lymphotoxin-beta which is independent of lympyhotoxin-alpha. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This splice variant (1) represents the longer transcript, encodes the longer isoform (a), and can form the heterotrimeric complex with lymphotoxin-alpha.