VCP (NM_007126) Human Untagged Clone

CAT#: SC125280

VCP (untagged)-Human valosin containing protein (VCP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VCP
• Synonyms: CDC48; p97; TERA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125280 sequence for NM_007126 edited (data generated by NextGen Sequencing)
  ATGGCTTCTGGAGCCGATTCAAAAGGTGATGACCTATCAACAGCCATTCTCAAACAGAAG
  AACCGTCCCAATCGGTTAATTGTTGATGAAGCCATCAATGAGGACAACAGTGTGGTGTCC
  TTGTCCCAGCCCAAGATGGATGAATTGCAGTTGTTCCGAGGTGACACAGTGTTGCTGAAA
  GGAAAGAAGAGACGAGAAGCTGTTTGCATCGTCCTTTCTGATGATACTTGTTCTGATGAG
  AAGATTCGGATGAATAGAGTTGTTCGGAATAACCTTCGTGTACGCCTAGGGGATGTCATC
  AGCATCCAGCCATGCCCTGATGTGAAGTACGGCAAACGTATCCATGTGCTGCCCATTGAT
  GACACAGTGGAAGGCATTACTGGTAATCTCTTCGAGGTATACCTTAAGCCGTACTTCCTG
  GAAGCGTATCGACCCATCCGGAAAGGAGACATTTTTCTTGTCCGTGGTGGGATGCGTGCT
  GTGGAGTTCAAAGTGGTGGAAACAGATCCTAGCCCTTATTGCATTGTTGCTCCAGACACA
  GTGATCCACTGCGAAGGGGAGCCTATCAAACGAGAGGATGAGGAAGAGTCCTTGAATGAA
  GTAGGGTATGATGACATTGGTGGCTGCAGGAAGCAGCTAGCTCAGATAAAGGAGATGGTG
  GAACTGCCCCTGAGACATCCTGCCCTCTTTAAGGCAATTGGTGTGAAGCCTCCTAGAGGA
  ATCCTGCTTTACGGACCTCCTGGAACAGGAAAGACCCTGATTGCTCGAGCTGTAGCAAAT
  GAGACTGGAGCCTTCTTCTTCTTGATCAATGGTCCTGAGATCATGAGCAAATTGGCTGGT
  GAGTCTGAGAGCAACCTTCGTAAAGCCTTTGAGGAGGCTGAGAAGAATGCTCCTGCCATC
  ATCTTCATTGATGAGCTAGATGCCATCGCTCCCAAAAGAGAGAAAACTCATGGCGAGGTG
  GAGCGGCGCATTGTATCACAGTTGTTGACCCTCATGGATGGCCTAAAGCAGAGGGCACAT
  GTGATTGTTATGGCAGCAACCAACAGACCCAACAGCATTGACCCAGCTCTACGGCGATTT
  GGTCGCTTTGACAGGGAGGTAGATATTGGAATTCCTGATGCTACAGGACGCTTAGAGATT
  CTTCAGATCCATACCAAGAACATGAAGCTGGCAGATGATGTGGACCTGGAACAGGTAGCC
  AATGAGACTCACGGGCATGTGGGTGCTGACTTAGCAGCCCTGTGCTCAGAGGCTGCTCTG
  CAAGCCATCCGCAAGAAGATGGATCTCATTGACCTAGAGGATGAGACCATTGATGCCGAG
  GTCATGAACTCTCTAGCAGTTACTATGGATGACTTCCGGTGGGCCTTGAGCCAGAGTAAC
  CCATCAGCACTGCGGGAAACCGTGGTAGAGGTGCCACAGGTAACCTGGGAAGACATCGGG
  GGCCTAGAGGATGTCAAACGTGAGCTACAGGAGCTGGTCCAGTATCCTGTGGAGCACCCA
  GACAAATTCCTGAAGTTTGGCATGACACCTTCCAAGGGAGTTCTGTTCTATGGACCTCCT
  GGCTGTGGGAAAACTTTGTTGGCCAAAGCCATTGCTAATGAATGCCAGGCCAACTTCATC
  TCCATCAAGGGTCCTGAGCTGCTCACCATGTGGTTTGGGGAGTCTGAGGCCAATGTCAGA
  GAAATCTTTGACAAGGCCCGCCAAGCTGCCCCCTGTGTGCTATTCTTTGATGAGCTGGAT
  TCGATTGCCAAGGCTCGTGGAGGTAACATTGGAGATGGTGGTGGGGCTGCTGACCGAGTC
  ATCAACCAGATCCTGACAGAAATGGATGGCATGTCCACAAAAAAAAATGTGTTCATCATT
  GGCGCTACCAACCGGCCTGACATCATTGATCCTGCCATCCTCAGACCTGGCCGTCTTGAT
  CAGCTCATCTACATCCCACTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAAC
  CTGCGCAAGTCCCCAGTTGCCAAGGATGTGGACTTGGAGTTCCTGGCTAAAATGACTAAT
  GGCTTCTCTGGAGCTGACCTGACAGAGATTTGCCAGCGTGCTTGCAAGCTGGCCATCCGT
  GAATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCATCAGCCATG
  GAGGTAGAAGAGGATGATCCAGTGCCTGAGATCCGTCGAGATCACTTTGAAGAAGCCATG
  CGCTTTGCGCGCCGTTCTGTCAGTGACAATGACATTCGGAAGTATGAGATGTTTGCCCAG
  ACCCTTCAGCAGAGTCGGGGCTTTGGCAGCTTCAGATTCCCTTCAGGGAACCAGGGTGGA
  GCTGGCCCCAGTCAGGGCAGTGGAGGCGGCACAGGTGGCAGTGTATACACAGAAGACAAT
  GATGATGACCTGTATGGCTAA
  
  Clone variation with respect to NM_007126.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007126 unedited
  NNGGGTCAGATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGTCGCTG
  CCGCTGCCGCTGCCACTGCCACTGCCACCTCGCGGATCAGGAGCCAGCGTTGTTCGCCCG
  ACGCCTCGCTGCCGGTGGGAGGAAGCGAGAGGGAAGCCGCTTGCGGGTTTGTCGCCGCTG
  CTCGCCCACCGCCTGGAAGAGCCGAGCCCCGGCCCAGTCGGTCGCTTGCCACCGCTCGTA
  GCCGTTACCCGCGGGCCGCCACAGCCGCCGGCCGGGAGAGGCGCGCGCCATGGCTTCTGG
  AGCCGATTCAAAAGGTGATGACCTATCAACAGCCATTCTCAAACAGAAGAACCGTCCCAA
  TCGGTTAATTGTTGATGAAGCCATCAATGAGGACAACAGTGTGGTGTCCTTGTCCCAGCC
  CAAGATGGATGAATTGCAGTTGTTCCGAGGTGACACAGTGTTGCTGAAAGGAAAGAAGAG
  ACGAGAAGCTGTTTGCATCGTCCTTTCTGATGATACTTGTTCTGATGAGAAGATTCGGAT
  GAATAGAGTTGTTCGGAATAACCTTCGTGTACGCCTAGGGGATGTCATCAGCATCCAGCC
  ATGCCCTGATGTGAAGTACGGCAAACGTATCCATGTGCTGCCCATTGATGACACAGTGGA
  AGGCATTACTGGTAATCTCTTCGAGGTATACCTTAAGCCGTACTTCCTGGAAGCGTATCG
  ACCCATCCGGAAAGGAGACATTNTTCTTGTCCGTGGTGGGATGCGTGCTGTGGAGTTCAA
  AGTGGTGGAAACAGATCCTAGCCCTTATTGCATTGNTGCTCCGACACAGTGATCACTGCG
  AAGGNGAGCCTATCANACGAGAGATGAAGGANAGTCCTTGATGAANTAGGTATGATGACA
  TGGNTGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007126 unedited
  NGGGCATTGNNAGTCGCTTTTCATTTATCGCTTTTGTTTTGTATTTTTGGCAACAGAAAA
  CCCCCTGTCCANAGTCAGACTGTAGCTGAACTGTTCAGACTGGAGAATGGAGCAGGCTGT
  GGGCGCACCCCTGGTCCCTCTCCTGGGCAAGCGCCCCCACCCCCAGGGAACAAGGTCCAG
  GCAGGCCAGCTCACTGCACGCTGGCCACCACCACTTAGCCATACAGGTCATCATCATTGT
  CTTCTGTGTATACACTGCCACCTGTGCCGCCTCCACTGCCCTGACTGGGGCCAGCTCCAC
  CCTGGTTCCCTGAAGGGAATCTGAAGCTGCCAAAGCCCCGACTCTGCTGAAGGGTCTGGG
  CAAACATCTCATACTTCCGAATGTCATTGTCACTGACAGAACGGCGCGCAAAGCGCATGG
  CTTCTTCAAAGTGATCTCGACGGATCTCAGGCACTGGATCATCCTCTTCTACCTCCATGG
  CTGATGGGTTTGTCTGCCTCTCTCGTTCTCGCCTAATCTCACTCTCGATGGATTCACGGA
  TGGCCAGCTTGCAAGCACGCTGGCAAATCTCTGTCAGGTCAGCTCCAGAGAAGCCATTAG
  TCATTTTAGCCAGGAACTCCAAGTCCACATCCTTGGCAACTGGGGACTTGCGCANGTTAG
  CCTTGAGGATGGGGCACACGGACTTCTCATCANGAAGTGGGATGTAGATGAGCTGATCAA
  GACNGCCAGGTCTGANGATGGCAGGATCAATGATGTCAGGCCCGTTGGTAGCGCCCATGA
  TGAACACATTTTTTTTTGTGGACATGCCATCCATTTNCTGTCAGGATCTGGNTTGATGAC
  TCGTCAGCAGCCCCACCACATCTTCAATGTACCCTCACGAGCCTTGGGGCATCGATCCAG
  CTCATCAAAGATAGCCCACAAGGGGCAGCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007126
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007126.2, NP_009057.1
• RefSeq Size: 3198 bp
• RefSeq ORF: 2421 bp
• Locus ID: 7415
• Cytogenetics: 9p13.3
• Domains: cdc48_N, AAA, AAA
• Gene Summary: 'This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients. [provided by RefSeq, Aug 2017]'