ACAT1 (NM_000019) Human Untagged Clone

CAT#: SC125282

ACAT1 (untagged)-Human acetyl-CoA acetyltransferase 1 (ACAT1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACAT1
• Synonyms: ACAT; MAT; T2; THIL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125282 sequence for NM_000019 edited (data generated by NextGen Sequencing)
  ATGGCTGTGCTGCCGGCACTTCTGCGCAGCGGCGCCCGCAGCCGCAGCCCCCTGCTCCGG
  AGGCTGGTGCAGGAAATAAGATATGTGGAACGGAGTTATGTATCAAAACCCACTTTGAAG
  GAAGTGGTCATAGTAAGTGCTACAAGAACACCCATTGGATCTTTTTTAGGCAGCCTTTCC
  TTGCTGCCAGCCACTAAGCTTGGTTCCATTGCAATTCAGGGAGCCATTGAAAAGGCAGGG
  ATTCCAAAAGAAGAAGTGAAAGAAGCATACATGGGTAATGTTCTACAAGGAGGTGAAGGA
  CAAGCTCCTACAAGGCAGGCAGTATTGGGTGCAGGCTTACCTATTTCTACTCCATGTACC
  ACCATAAACAAAGTTTGTGCTTCAGGAATGAAAGCCATCATGATGGCCTCTCAAAGTCTT
  ATGTGTGGACATCAGGATGTGATGGTGGCAGGTGGGATGGAGAGCATGTCCAATGTTCCA
  TATGTAATGAACAGAGGATCAACACCATATGGTGGGGTAAAGCTTGAAGATTTGATTGTA
  AAAGACGGGCTAACTGATGTCTACAATAAAATTCATATGGGCAGCTGTGCTGAGAATACA
  GCAAAGAAGCTGAATATTGCACGAAATGAACAGGACGCTTATGCTATTAATTCTTATACC
  AGAAGTAAAGCAGCATGGGAAGCTGGGAAATTTGGAAATGAAGTTATTCCTGTCACAGTT
  ACAGTAAAAGGTCAACCAGATGTAGTGGTGAAAGAAGATGAAGAATATAAACGTGTTGAT
  TTTAGCAAAGTTCCAAAGCTGAAGACAGTTTTCCAGAAAGAAAATGGCACAGTAACAGCT
  GCCAATGCCAGTACACTGAATGATGGAGCAGCTGCTCTGGTTCTCATGACGGCAGATGCA
  GCGAAGAGGCTCAATGTTACACCACTGGCAAGAATAGTAGCATTTGCTGACGCTGCTGTA
  GAACCTATTGATTTTCCAATTGCTCCTGTATATGCTGCATCTATGGTTCTTAAAGATGTG
  GGATTGAAAAAAGAAGATATTGCAATGTGGGAAGTAAATGAAGCCTTTAGTCTGGTTGTA
  CTAGCAAACATTAAAATGTTGGAGATTGATCCCCAAAAAGTGAATATCAATGGAGGAGCT
  GTTTCTCTGGGACATCCAATTGGGATGTCTGGAGCCAGGATTGTTGGTCATTTGACTCAT
  GCCTTGAAGCAAGGAGAATACGGTCTTGCCAGTATTTGCAATGGAGGAGGAGGTGCTTCT
  GCCATGCTAATTCAGAAGCTGTAG
  
  Clone variation with respect to NM_000019.3
  13 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000019 unedited
  ATTTTGTATCCGACTTCTATAGGCGGCCGCGCAATTCGCACGAGTGGAGCCGATACTCAT
  CCCACTGCGACCATGGCTGTGCTGCCGGCACTTCTGCGCATCGGCGCCCGCAGCCGCAGC
  CCCCTGCTCCGGAGGCTGGTGCAGGAAATAAGATATGTGGAACGGAGTTATGTATCAAAA
  CCCACTTTGAAGGAAGTGGTCATAGTAAGTGCTACAAGAACACCCATTGGATCTTTTTTA
  AGCAGCCTTTCCTTGCTGCCAGCCACTAAGCTTGGTTCCATTGCAATTCAAGGAGCCATT
  GAAAAGGCAGGGATTCCAAAAGAAGAAGTGAAAGAAGCATACATGGGTAATGTTCTACAA
  AGAGGTGAAAGACAAGCTCCTACAAAGCAAGCAGTATTGGGTGCAAGCTTACCTATTTCT
  ACTCCATGTACCACCATAAACAAAGTTTGTGCTTCAAGAATGAAAGCCATCATGATGGCC
  TCTCAAAGTCTTATGTGTGGACATCAAGATGTGATGGTGGCAGGTGGGATGGATAGCATG
  TCCAATGTTCCATATGTAATGAACAGATGATCAACACCATATGGTGGGGTAAAGCTTGAA
  GATTTGATTGCTAAAGACGGGCTAACTGATGTCTACAATAAAATTCATATGGGCAGCTGT
  GCTGAGAATACAGCANAGAATCTGAATATTGCACGAAATGAACAGGACGGCTATGCTATT
  AATTCCTATACCANAAGTAAAGCAGCATGGGAAGCTGGGAAATTTGCAAATGAAGNTATT
  CCTGTCACAGTTACAGTAAAAAGTCAACCANATGTATCGGCAGAAAGAGAATGAGAATAT
  AAACGCGTGATCTTAACAAGTTCCAAAGCTGAAGACAGTCTTCCCGAAAGAAAATGCCCA
  GTNACAGCTGTCAATTGCCTNACACTGAATGATGGAGCAACTGCTCCTGTTCTCATGACG
  CANATCCCGGAATAGCTCATGTTCCCACTGCCAAAATAAACCTGGCGGCCCGTCGTAAAT
  CTATGATTTCAATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_000019 unedited
  AGNCACATAACAAGNCCTCAAAATTGGTGATGATTACAGAGTTTTTGAAAAACTCTACTC
  TTCCTCTGAATCTGAAGAGCAATCTAATCTATTTCTCCCATATATTGTCATTTTGCTCCT
  TCAAAAGATCTACAATACATTATTTGCCAACAGATAATGTGTACACAATGACCTTTACTG
  CCCCCTCCTCTTCCTGAGGGGGAGTTTTCCCTCTTCAGAGCTGCAATTGAGGGTAGTGTG
  CATAGCCCCTGCCTGTAGCGTTTTAATTAATTTTTTCCCAGATAAAATGCAATGCGAGAT
  ACTTCTGTTACAGTCTGTCCTGCTTAAGACAGGCATTGTCGTGTATTGGAAGTGGTGATC
  TGTTCCCTTTTTTATATATCCATTCAGCATATCAGTTTCTACCCTCCCTTCCACTCTCTA
  TGCTGCTATAGTAATTCAGAGCCCAAACTCCAGGGTTTTCTGCTTGACCCTCCCCCAACC
  CTGAATGTTGAGGTTATAAGAAAATGCCCCCCCTCTAGACCCTGAGGACATGGGGCTTCT
  GTCCTTGTTTCTAATACTGCTGGCCCACACTGGGGTTTTGGAGTGATTATATTAAGCCAC
  TGCTAAGTTTGTCCCAAAATACCCACTTCTAACTGGAAAAAAAAAATAATTTTAATTTAC
  CCGGAATTTACCTAAATGTAAATTAACCTTANAAAAGCCATTCCTTTGACCCTTTAAAAA
  TCCTAAAAAAATTAAAGGGGTTCTCAACAATGGAAACCTTCTGCCCCCTATTTTAAGCCC
  CTAATTTTCCCCCTTGGGATTTTTCCCCTATTCCGAAAAGGTAGACCTCCAAAGGCAAAA
  TGTTTCAAACTTTTCAGGGTTCCCCGAAAAAACCCCAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000019
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000019.2, NP_000010.1
• RefSeq Size: 1518 bp
• RefSeq ORF: 1284 bp
• Locus ID: 38
• Cytogenetics: 11q22.3
• Domains: thiolase
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Fatty acid metabolism, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Tryptophan metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]