DDX5 (NM_004396) Human Untagged Clone

CAT#: SC125285

DDX5 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX5
• Synonyms: G17P1; HLR1; HUMP68; p68
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125285 sequence for NM_004396 edited (data generated by NextGen Sequencing)
  ATGTCGGGTTATTCGAGTGACCGAGACCGCGGCCGGGACCGAGGGTTTGGTGCACCTCGA
  TTTGGAGGAAGTAGGGCAGGGCCCTTATCTGGAAAGAAGTTTGGAAACCCTGGGGAGAAA
  TTAGTTAAAAAGAAGTGGAATCTTGATGAGCTGCCTAAATTTGAGAAGAATTTTTATCAA
  GAGCACCCTGATTTGGCTAGGCGCACAGCACAAGAGGTGGAAACATACAGAAGAAGCAAG
  GAAATTACAGTTAGAGGTCACAACTGCCCGAAGCCAGTTCTAAATTTTTATGAAGCCAAT
  TTCCCTGCAAATGTCATGGATGTTATTGCAAGACAGAATTTCACTGAACCCACTGCTATT
  CAAGCTCAGGGATGGCCAGTTGCTCTAAGTGGATTGGATATGGTTGGAGTGGCACAGACT
  GGATCTGGGAAAACATTGTCTTATTTGCTTCCTGCCATTGTCCACATCAATCATCAGCCA
  TTCCTAGAGAGAGGCGATGGGCCTATTTGTTTGGTGCTGGCACCAACTCGGGAACTGGCC
  CAACAGGTGCAGCAAGTAGCTGCTGAATATTGTAGAGCATGTCGCTTGAAGTCTACTTGT
  ATCTACGGTGGTGCTCCTAAGGGACCACAAATACGTGATTTGGAGAGAGGTGTGGAAATC
  TGTATTGCAACACCTGGAAGACTGATTGACTTTTTAGAGTGTGGAAAAACCAATCTGAGA
  AGAACAACCTACCTTGTCCTTGATGAAGCAGATAGAATGCTTGATATGGGCTTTGAACCC
  CAAATAAGGAAGATTGTGGATCAAATAAGACCTGATAGGCAAACTCTAATGTGGAGTGCG
  ACTTGGCCAAAAGAAGTAAGACAGCTTGCTGAAGATTTCCTGAAAGACTATATTCATATA
  AACATTGGTGCACTTGAACTGAGTGCAAACCACAACATTCTTCAGATTGTGGATGTGTGT
  CATGACGTAGAAAAGGATGAAAAACTTATTCGTCTAATGGAAGAGATCATGAGTGAGAAG
  GAGAATAAAACCATTGTTTTTGTGGAAACCAAAAGAAGATGTGATGAGCTTACCAGAAAA
  ATGAGGAGAGATGGGTGGCCTGCCATGGGTATCCATGGTGACAAGAGTCAACAAGAGCGT
  GACTGGGTTCTAAATGAATTCAAACATGGAAAAGCTCCTATTCTGATTGCTACAGATGTG
  GCCTCCAGAGGGCTAGATGTGGAAGATGTGAAATTTGTCATCAATTATGACTACCCTAAC
  TCCTCAGAGGATTATATTCATCGAATTGGAAGAACTGCTCGCAGTACCAAAACAGGCACA
  GCATACACTTTCTTTACACCTAATAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTT
  CGTGAAGCTAATCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACAGAGGTTCA
  GGTCGTTCCAGGGGTAGAGGAGGCATGAAGGATGACCGTCGGGACAGATACTCTGCGGGC
  AAAAGGGGTGGATTTAATACCTTTAGAGACAGGGAAAATTATGACAGAGGTTACTCTAGC
  CTGCTTAAAAGAGATTTTGGGGCAAAAACTCAGAATGGTGTTTACAGTGCTGCAAATTAC
  ACCAATGGGAGCTTTGGAAGTAATTTTGTGTCTGCTGGTATACAGACCAGTTTTAGGACT
  GGTAATCCAACAGGGACTTACCAGAATGGTTATGATAGCACTCAGCAATACGGAAGTAAT
  GTTCCAAATATGCACAATGGTATGAACCAACAGGCATATGCATATCCTGCTACTGCAGCT
  GCACCTATGATTGGTTATCCAATGCCAACAGGATATTCCCAATAA
  
  Clone variation with respect to NM_004396.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004396 unedited
  GGGGGCGGGGGCNCCCNNNNNNTTTTNCNNNNNCGGTTCAGATTTGTATACAACTCATAT
  AGGCGGCCGCGNAATTCGCACGAGCCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATC
  GGTGTCCTTCCTCCGCTGCCGCCCCCGCAAGGCTTCGCCGTCATCGAGGCCATTTCCAGC
  GACTTGTCGCACGCTTTTCTATATACTTCGTTCCCCGCCAACCGCAACCATTGACGCCAT
  GTCGGGTTATTCGAGTGACCGAGACCGCGGCCGGGACCGAGGGTTTGGTGCACCTCGATT
  TGGAGGAAGTAGGGCAGGGCCCTTATCTGGAAAGAAGTTTGGAAACCCTGGGGAGAAATT
  AGTTAAAAAGAAGTGGAATCTTGATGAGCTGCCTAAATTTGAGAAGAATTTTTATCAAGA
  GCACCCTGATTTGGCTAGGCGCACAGCACAAGAGGTGGAAACATACAGAAGAAGCAAGGA
  AATTACAGTTAGAGGTCACAACTGCCCGAAGCCAGTTCTAAATTTTTATGAAGCCAATTT
  CCCTGCAAATGTCATGGATGTTATTGCAAGACAGAATTTCACTGAACCCACTGCTATTCA
  AGCTCAGGGATGGCCAGTTGCTCTAAGTGGATTGGATATGGTTGGAGTGGCACAGACTGG
  ATCTGGGAAAACATTGTCTTATTTGCTTCCTGCCATTGTCCACATCAATCATCAGCCATT
  CCTAGAGAGAGGCGATGGGCCTATTTGTTTGGTGCTGGCACCAACTCGGGAACTGGCCCA
  ACAGGTGCAGCAAGTAGCTGCTGAATATTGTAGAGCATGTCGCTTGAAGTCTACTTGTAT
  CTACNGTGGTGCTCCTAAGGGACCCAAATACNTGATTGGGAGAGAGGTGTGAAATCTGTA
  TTGCACACCTGAAGAAACTGATGACTTTTANATNGTGAANAACCATCTGAGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004396 unedited
  NNNNCCCCCTCCTGGGCCATGGTGATGGCACTTGCCAGGNNCCAGNGANGAGCACTGGGG
  NAGGGAGTCACAGGGCATGCCACCCGGGTACTGTTCAGGAAACAGCTATGACCGCGGCCG
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCAGTCTTCAAATATTTTTTATTGGAA
  GGCCATGCATTGCCTTCATTTATTGTATTTCAAATCACTGTACATTTACTTTTGTGAAAA
  CACTGCCTGCATTTTCTAGTACAAAAAACACCTAAAAATTGTTTCAGGAATGTAGAGAAA
  TATCCAACTTAAATAGCGAAAAAGTGCACCATAATTACTGCTGCACTGCAGTCATTTCTG
  CAATTCCATGTTTCTTAAAAACTATCTTGTCAGATAACACACATATTAAGAGCAATTATG
  AAAAACAGACTTTACATATACTTCTAAAGTCTTATTGGGAATATCCTGTTGGCATTGGAA
  TACCAATCATAGGTGCAGCTGCAGTAACAGGATATGCATATGCCCTGTTGGTTCATACCA
  TTGGGCTATTTTGAACATTACTTCCGTATGGCTGAGTGCTATCATAACCATTCTGTAAGT
  CCCTGTTGGATACCATTCCAAAACTGGGCTGGTTCCAACCAGACCCAAATTACTTTCAAA
  GCCTTCCATGGGTGAATTTGGAGCACTGAAAAACCCTTTTTGATTTTTTGCCCAAAATCT
  TTTTAAGCGAGCTAAAATAACCTCTGGCAAAATTTTCCCCGGGCTTAAAGGGTTTAAATC
  CCCCCTTTTGGCCCCAAAAATTTTTTTCCCCAAGGTAATCCTTAGGCCCCCCTTACCCGG
  GAACGACCTGAACCTTGTTTTTCACCAAATGAAACACTTGGAAATAATGCCTTGATACCC
  TCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004396
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004396.2, NP_004387.1
• RefSeq Size: 2325 bp
• RefSeq ORF: 1845 bp
• Locus ID: 1655
• Cytogenetics: 17q23.3
• Domains: DEAD, helicase_C
• Protein Pathways: Spliceosome
• Gene Summary: 'This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2017]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 through 3 encode the same isoform (a).