MARCKS (NM_002356) Human Untagged Clone

CAT#: SC125313

MARCKS (untagged)-Human myristoylated alanine-rich protein kinase C substrate (MARCKS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARCKS
• Synonyms: 80K-L; MACS; PKCSL; PRKCSL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125313 sequence for NM_002356 edited (data generated by NextGen Sequencing)
  ATGGGTGCCCAGTTCTCCAAGACCGCAGCGAAGGGAGAAGCCGCCGCGGAGAGGCCTGGG
  GAGGCGGCTGTGGCCTCGTCGCCTTCCAAAGCGAACGGACAGGAGAATGGCCACGTGAAG
  GTAAACGGCGACGCTTCGCCCGCGGCCGCCGAGTCGGGCGCCAAGGAGGAGCTGCAGGCC
  AACGGCAGCGCCCCGGCCGCCGACAAGGAGGAGCCCGCGGCCGCCGGGAGCGGGGCGGCG
  TCGCCCTCCGCGGCCGAGAAAGGTGAGCCGGCCGCCGCCGCTGCCCCCGAGGCCGGGGCC
  AGCCCGGTAGAGAAGGAGGCCCCCGCGGAAGGCGAGGCTGCCGAGCCCGGCTCGCCCACG
  GCCGCGGAGGGAGAGGCCGCGTCGGCCGCCTCCTCGACTTCTTCGCCCAAGGCCGAGGAC
  GGGGCCACGCCCTCGCCCAGCAACGAGACCCCGAAAAAAAAAAAGAAGCGCTTTTCCTTC
  AAGAAGTCTTTCAAGCTGAGCGGCTTCTCCTTCAAGAAGAACAAGAAGGAGGCTGGAGAA
  GGCGGTGAGGCTGAGGCGCCCGCTGCCGAAGGCGGCAAGGACGAGGCCGCCGGGGGCGCA
  GCTGCGGCCGCCGCCGAGGCGGGCGCGGCCTCCGGGGAGCAGGCAGCGGCGCCGGGCGAG
  GAGGCGGCAGCGGGCGAGGAGGGGGCGGCGGGTGGCGACCCGCAGGAGGCCAAGCCCCAG
  GAGGCCGCTGTCGCGCCAGAGAAGCCGCCCGCCAGCGACGAGACCAAGGCCGCCGAGGAG
  CCCAGCAAGGTGGAGGAGAAAAAGGCCGAGGAGGCCGGGGCCAGCGCCGCCGCCTGCGAG
  GCCCCCTCCGCCGCCGGGCCCGGCGCGCCCCCGGAGCAGGAGGCAGCCCCCGCGGAGGAG
  CCCGCGGCCGCCGCAGCCTCGTCAGCCTGCGCAGCCCCCTCACAGGAGGCCCAGCCCGAG
  TGCAGTCCAGAAGCCCCCCCAGCGGAGGCGGCAGAGTAA
  
  Clone variation with respect to NM_002356.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002356 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCATTTTCCTCTTGT
  CTACAGTGCGGCTACAAATCTGGGATTTTTTTATTACTTCTTTTTTTTTCGAACTACACT
  TGGGCTCCTTTTTTGTGCTCGACTTTTCCACCCTTTTTCCCTCCCTCCTGTGCTGCTGCT
  TTTTGATCTCTTCGACTAAAATTTTTTTATCCGGAGTGTATTTAATCGGTTCTGTTCTGT
  CCTCTCCACCACCCCCACCCCCCTCCCTCCGGTGTGTGTGCCGCTGCCGCTGTTGCCGCC
  GCCGCTGCTGCTGCTCGCCCCGTCGTTACACCAACCCGAGGCTCTTTGTTTCCCCTCTTG
  GATCTGTTGAGTTTCTTTGTTGAAGAAGCCAGCATGGGTGCCCAGTTCTCCAAGACCGCA
  GCGAAGGGAGAAGCCGCCGCGGAGAGGCCTGGGGAGGCGGCTGTGGCCTCGTCGCCTTCC
  AAAGCGAACGGACAGGAGAATGGCCACGTGAAGGTAAACGGCGACGCTTCGCCCGCGGCC
  GCCGAGTCGGGCGCCAAGGAGGAGCTGCAGGCCAACGGCAGCGCCCCGGCCGCCGACAAG
  GAGGAGCCCGCGGCCGCCGGGAGCGGGGCGGCGTCGCCCTCCGCGGCCGAGAAAGGTGAG
  CCGGCCGCCGCCGCTGCCCCCGAGGCCGTGGCCAGCCCGGTAGAGAAAGAGGCCCCCGCG
  GAGGCGAAGCTGCCGACCCCGCTCGCCCACGTCGCGGAGGGAGAGGCCGCGTCCGCCGCC
  TTCTCGACTTCTTCGCCCAGGGCCGAGGACGGGCCCCGCCCTCGCCCAGCACGAGACCCG
  GAAAAAAAAAAGAAGCGCTTTTCCTTCAGGAAGTCTTTCAAGCTGAGCGGGCTTTTCCTT
  CAGAAGACCANAAAGGAGCTTGGAAAGGCGGGGAGGTTTTAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002356 unedited
  CTCTGCACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTACATTTTATT
  CACATTTATTTTTCGCTTTTAGTGTGCTCACAGAAAATTAGAACACCTTAAGCAGGAGTT
  TAATAGCAATTTTTGTAAGCAAAGTTACATTCCATCTCTAAGTCAAATTGGTCAAAGCTT
  CTCCAGTATTTACAAAACATGATAGACAAGATGCTACACAAAACCATTGCATCTGAAGAT
  TTTTTTTCCTTTATTCTCAAAGACGACTGGAAAAGAAAGCATTATCTGCTGTAATCAAAA
  ACATACCACAGTATAAACAGTAACCATTCCACTTATCACAGCTTGGTTGAGTTTAAAATT
  TGTGTTTTAAAAGGTCCAAGATGACTGCAGTTTTACAAAAATGGGCAGGGTGGAAAGTTG
  CAAACTTCATGTGCTTCTGGATATCAAGATTTGTTTTTATACAATAGCCACAGTTAAAAA
  CACCCTGCTGGTAATACATAATTACACTTTATTAAGGTCATAAACCAGCAATAAACAATA
  AAGCCTATACAACTTGTAGTTCTACTTAATCACTGACTGGTACAGCTAACATGAGATAAG
  TGAAAAGTTCCTATGGTTTAAATGAACTGCTAAGACTATGATCGTCTCTTTTTTTTTAAA
  TCTGGCCCTTGGAGTCTTTTCTTTTTTCCCCTTTCCTTCTTACCTAAGACTTGTAGGGCC
  GGAAACCTGCCTCACAAAAACACGGTCATAACTTTACTTTAAAAAAAAAAAAAAAGACAC
  GCTTTACACATTTTCAGGGGCCCCTATTTGGCAATGGTAGGCCCCCTCTATTTGCTCTTG
  GGACCCCTATCACCCATTTGAATTCCCTTCTTGACCCCACTCTCCTCATATATGCCAGGC
  CTATATGACAAAAAGTTTCCCTTTTGATCAAGAGCAAGTTGCCCAACCTTATCCGTGCCC
  AAAATAAAACAAACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002356
• Insert Size: 2560 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002356.4, NP_002347.4
• RefSeq Size: 2589 bp
• RefSeq ORF: 999 bp
• Locus ID: 4082
• Cytogenetics: 6q21
• Domains: MARCKS
• Protein Families: Druggable Genome
• Protein Pathways: Fc gamma R-mediated phagocytosis
• Gene Summary: 'The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]'