Nucleolin (NCL) (NM_005381) Human Untagged Clone

CAT#: SC125320

NCL (untagged)-Human nucleolin (NCL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCL
• Synonyms: C23; Nsr1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125320 sequence for NM_005381 edited (data generated by NextGen Sequencing)
  ATGGTGAAGCTCGCGAAGGCAGGTAAAAATCAAGGTGACCCCAAGAAAATGGCTCCTCCT
  CCAAAGGAGGTAGAAGAAGATAGTGAAGATGAGGAAATGTCAGAAGATGAAGAAGATGAT
  AGCAGTGGAGAAGAGGTCGTCATACCTCAGAAGAAAGGCAAGAAGGCTGCTGCAACCTCA
  GCAAAGAAGGTGGTCGTTTCCCCAACAAAAAAGGTTGCAGTTGCCACACCAGCCAAGAAA
  GCAGCTGTCACTCCAGGCAAAAAGGCAGCAGCAACACCTGCCAAGAAGACAGTTACACCA
  GCCAAAGCAGTTACCACACCTGGCAAGAAGGGAGCCACACCAGGCAAAGCATTGGTAGCA
  ACTCCTGGTAAGAAGGGTGCTGCCATCCCAGCCAAGGGGGCAAAGAATGGCAAGAATGCC
  AAGAAGGAAGACAGTGATGAAGAGGAGGATGATGACAGTGAGGAGGATGAGGAGGATGAC
  GAGGACGAGGATGAGGATGAAGATGAAATTGAACCAGCAGCGATGAAAGCAGCAGCTGCT
  GCCCCTGCCTCAGAGGATGAGGACGATGAGGATGACGAAGATGATGAGGATGACGATGAC
  GATGAGGAAGATGACTCTGAAGAAGAAGCTATGGAGACTACACCAGCCAAAGGAAAGAAA
  GCTGCAAAAGTTGTTCCTGTGAAAGCCAAGAACGTGGCTGAGGATGAAGATGAAGAAGAG
  GATGATGAGGACGAGGATGACGACGACGACGAAGATGATGAAGATGATGATGATGAAGAT
  GATGAGGAGGAGGAAGAAGAGGAGGAGGAAGAGCCTGTCAAAGAAGCACCTGGAAAACGA
  AAGAAGGAAATGGCCAAACAGAAAGCAGCTCCTGAAGCCAAGAAACAGAAAGTGGAAGGC
  ACAGAACCGACTACGGCTTTCAATCTCTTTGTTGGAAACCTAAACTTTAACAAATCTGCT
  CCTGAATTAAAAACTGGTATCAGCGATGTTTTTGCTAAAAATGATCTTGCTGTTGTGGAT
  GTCAGAATTGGTATGACTAGGAAATTTGGTTATGTGGATTTTGAATCTGCTGAAGACCTG
  GAGAAAGCGTTGGAACTCACTGGTTTGAAAGTCTTTGGCAATGAAATTAAACTAGAGAAA
  CCAAAAGGAAAAGACAGTAAGAAAGAGCGAGATGCGAGAACACTTTTGGCTAAAAATCTC
  CCTTACAAAGTCACTCAGGATGAATTGAAAGAAGTGTTTGAAGATGCTGCGGAGATCAGA
  TTAGTCAGCAAGGATGGGAAAAGTAAAGGGATTGCTTATATTGAATTTAAGACAGAAGCT
  GATGCAGAGAAAACCTTTGAAGAAAAGCAGGGAACAGAGATCGATGGGCGATCTATTTCC
  CTGTACTATACTGGAGAGAAAGGTCAAAATCAAGACTATAGAGGTGGAAAGAATAGCACT
  TGGAGTGGTGAATCAAAAACTCTGGTTTTAAGCAACCTCTCCTACAGTGCAACAGAAGAA
  ACTCTTCAGGAAGTATTTGAGAAAGCAACTTTTATCAAAGTACCCCAGAACCAAAATGGC
  AAATCTAAAGGGTATGCATTTATAGAGTTTGCTTCATTCGAAGACGCTAAAGAAGCTTTA
  AATTCCTGTAATAAAAGGGAAATTGAGGGCAGAGCAATCAGGCTGGAGTTGCAAGGACCC
  AGGGGATCACCTAATGCCAGAAGCCAGCCATCCAAAACTCTGTTTGTCAAAGGCCTGTCT
  GAGGATACCACTGAAGAGACATTAAAGGAGTCATTTGACGGCTCCGTTCGGGCAAGGATA
  GTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGGTTTTGTAGACTTCAACAGTGAG
  GAGGATGCCAAAGCTGCCAAGGAGGCCATGGAAGACGGTGAAATTGATGGAAATAAAGTT
  ACCTTGGACTGGGCCAAACCTAAGGGTGAAGGTGGCTTCGGGGGTCGTGGTGGAGGCAGA
  GGCGGCTTTGGAGGACGAGGTGGTGGTAGAGGAGGCCGAGGAGGATTTGGTGGCAGAGGC
  CGGGGAGGCTTTGGAGGGCGAGGAGGCTTCCGAGGAGGCAGAGGAGGAGGAGGTGACCAC
  AAGCCACAAGGAAAGAAGACGAAGTTTGAATAG
  
  Clone variation with respect to NM_005381.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005381 unedited
  ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCGAGCTCTCGC
  TGGCCTTCGGGTGTACGTGCTCCGGGATCTTCAGCACCCGCGGCCGCCATCGCCGTCGCT
  TGGCTTCTTCTGGACTCATCTGCGCCACTTGTCCGCTTCACACTCCGCCGCCATCATGGT
  GAAGCTCGCGAAGGCAGGTAAAAATCAAGGTGACCCCAAGAAAATGGCTCCTCCTCCAAA
  GGAGGTAGAAGAAGATAGTGAAGATGAGGAAATGTCAGAAGATGAAGAAGATGATAGCAG
  TGGAGAAGAGGTCGTCATACCTCAGAAGAAAGGCAAGAAGGCTGCTGCAACCTCAGCAAA
  GAAGGTGGTCGTTTCCCCAACAAAAAAGGTTGCAGTTGCCACACCAGCCAAGAAAGCAGC
  TGTCACTCCAGGCAAAAAGGCAGCAGCAACACCTGCCAAGAAGACAGTTACACCAGCCAA
  AGCAGTTACCACACCTGGCAAGAAGGGAGCCACACCAGGCAAAGCATTGGTAGCAACTCC
  TGGTAAGAAGGGTGCTGCCATCCCAGCCAAGGGGGCAAAGAATGGCAAGAATGCCAAGAA
  GGAAGACAGTGATGAAGAGGAGGATGATGACAGTGAGGAGGATGAGGAGGATGACGAGGA
  CGAGGATGAGGATGAAGATGAAATTGAACCAGCAGCGATGAAAGCAGCAGCTGGCTGCCC
  CCTGCCTCANAGGATGAGGACGATGANGATGACGAANATGATGAGGATGACGATGACGAT
  GAGGAAGATGACTCTGAAGAAGAGCTTGGNAGACTACACCAGCCAAGGAAAGAAAGCTGC
  AAAGNTGNTCTGTGAAGCCNANAACGTGCTGAGGATGAAAAGAAAANAAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005381 unedited
  AATAGCTAGNACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGCACC
  AAAGTTGACTTTTAATTTGTCCTAAAGCCGCTGAAGCAAAAACCATGATAAAACATTCTG
  CTTTCTTTTCTTTTACAACCCCACGAACGCAAAAAAAAAAAAAACAAAAACAAAACAAAA
  AAAAGAAACAACAACAAAACCCAAACTATTTGTAGGAAAAAATGGTTTTGTACATGGGAT
  GAAACAATATAAATTCAAAACTTACAGATAAGGGTTAGCTCTATCACTCAACTCTTTAAA
  AAGTTTATATGAATATCCAGTCAAAACCAACACGGTATTGCCCTTGAAATGTTAACTAGA
  CGGATTTCCAAGGAGACCACAGGACTGTATACTGTCTTGGAATGTCCTCAGAAGGCTCTG
  TCATTGATCAGGTAACAGTAAAAACCCCAGAGTCCTTTCTTTCAAATGGAAAAGGGAAAG
  CAGAGGGACAGAAGCTATTCAAACTTCGTCTTCTTTCCTTGTGGCTTGTGGTCACCTCCT
  CCTCCTCTGCCTCCTCGGAAGCCTCCTCGCCCTCCAAAGCCTCCCCGGCCTCTGCCACCA
  AATCCTCCTCGGCCTCCTCTACCACCACCTCGTCCTCCAAAGCCGCCTCTGCCTCCACCA
  CGACCCCCGAAGCCACCTTCACCCTTAGGTTTGGCCCAGTCCAAGTAACTTTATTTCCAT
  CNTTTCACCGTCTTCCATGGCCTCCCTGGGAGCTTTGGCATCCCCCTCACTGTTGAAGTC
  TACAAAACCAAACCCTTTGGAGGACCCAGTTTCCCGGGCAGTACTATCCTTGCCCGAACG
  GACCCGCAAAAGACTCCTTAAAGGCCCTCAGGGGTACCCTCAAAAAGGCCTTGACAAAAG
  ATTTTTGGAGGGCGGGCTCGGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005381
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005381.2, NP_005372.2
• RefSeq Size: 2732 bp
• RefSeq ORF: 2133 bp
• Locus ID: 4691
• Cytogenetics: 2q37.1
• Domains: RRM
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Pathogenic Escherichia coli infection
• Gene Summary: 'Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]'