Macrophage Scavenger Receptor I (MSR1) (NM_138715) Human Untagged Clone

CAT#: SC125331

MSR1 (untagged)-Human macrophage scavenger receptor 1 (MSR1), transcript variant SR-AI

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSR1
• Synonyms: CD204; phSR1; phSR2; SCARA1; SR-A; SR-AI; SR-AII; SR-AIII; SRA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125331 sequence for NM_138715 edited (data generated by NextGen Sequencing)
  ATGGAGCAGTGGGATCACTTTCACAATCAACAGGAGGACACTGATAGCTGCTCCGAATCT
  GTGAAATTTGATGCTCGCTCAATGACAGCTTTGCTTCCTCCGAATCCTAAAAACAGCCCT
  TCCCTTCAAGAGAAACTGAAGTCCTTCAAAGCTGCACTGATTGCCCTTTACCTCCTCGTG
  TTTGCAGTTCTCATCCCTCTCATTGGAATAGTGGCAGCTCAACTCCTGAAGTGGGAAACG
  AAGAATTGCTCAGTTAGTTCAACTAATGCAAATGATATAACTCAAAGTCTCACGGGAAAA
  GGAAATGACAGCGAAGAGGAAATGAGATTTCAAGAAGTCTTTATGGAACACATGAGCAAC
  ATGGAGAAGAGAATCCAGCATATTTTAGACATGGAAGCCAACCTCATGGACACAGAGCAT
  TTCCAAAATTTCAGCATGACAACTGATCAAAGATTTAATGACATTCTTCTGCAGCTAAGT
  ACCTTGTTTTCCTCAGTCCAGGGACATGGGAATGCAATAGATGAAATCTCCAAGTCCTTA
  ATAAGTTTGAATACCACATTGCTTGATTTGCAGCTCAACATAGAAAATCTGAATGGCAAA
  ATCCAAGAGAATACCTTCAAACAACAAGAGGAAATCAGTAAATTAGAGGAGCGTGTTTAC
  AATGTATCAGCAGAAATTATGGCTATGAAAGAAGAACAAGTGCATTTGGAACAGGAAATA
  AAAGGAGAAGTGAAAGTACTGAATAACATCACTAATGATCTCAGACTGAAAGATTGGGAA
  CATTCTCAGACCTTGAGAAATATCACTTTAATTCAAGGTCCTCCTGGACCCCCGGGTGAA
  AAAGGAGATCGAGGTCCCACTGGAGAAAGTGGTCCACGAGGATTTCCAGGTCCAATAGGT
  CCTCCGGGTCTTAAAGGTGATCGGGGAGCAATTGGCTTTCCTGGAAGTCGAGGACTCCCA
  GGATATGCCGGAAGGCCAGGAAATTCTGGACCAAAAGGCCAGAAAGGGGAAAAGGGGAGT
  GGAAACACATTAACTCCATTTACGAAAGTTCGACTGGTCGGTGGGAGCGGCCCTCACGAG
  GGGAGAGTGGAGATACTCCACAGCGGCCAGTGGGGTACAATTTGTGACGATCGCTGGGAA
  GTGCGCGTTGGACAGGTCGTCTGTAGGAGCTTGGGATACCCAGGTGTTCAAGCCGTGCAC
  AAGGCAGCTCACTTTGGACAAGGTACTGGTCCAATATGGCTGAATGAAGTGTTTTGTTTT
  GGGAGAGAATCATCTATTGAAGAATGTAAAATTCGGCAATGGGGGACAAGAGCCTGTTCA
  CATTCTGAAGATGCTGGAGTCACTTGCACTTTATAA
  
  Clone variation with respect to NM_138715.2
  1086 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138715 unedited
  GGGGGTTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGATTCGCACGAGGTCATTT
  CCTTTCTTCATGTCCAGAGCTGAAATACTATGAGATAAAGATTTTAGGTTTCAATTGTAA
  AGAGAGAGAAGTGGATAAATCAGTGCTGCTTTCTTTAGGACGAAAGAAGTATGGAGCAGT
  GGGATCACTTTCACAATCAACAGGAGGACACTGATAGCTGCTCCGAATCTGTGAAATTTG
  ATGCTCGCTCAATGACAGCTTTGCTTCCTCCGAATCCTAAAAACAGCCCTTCCCTTCAAG
  AGAAACTGAAGTCCTTCAAAGCTGCACTGATTGCCCTTTACCTCCTCGTGTTTGCAGTTC
  TCATCCCTCTCATTGGAATAGTGGCAGCTCAACTCCTGAAGTGGGAAACGAAGAATTGCT
  CAGTTAGTTCAACTAATGCAAATGATATAACTCAAAGTCTCACGGGAAAAGGAAATGACA
  GCGAAGAGGAAATGAGATTTCAAGAAGTCTTTATGGAACACATGAGCAACATGGAGAAGA
  GAATCCAGCATATTTTAGACATGGAAGCCAACCTCATGGACACAGAGCATTTCCAAAATT
  TCAGCATGACAACTGATCAAAGATTTAATGACATTCTTCTGCAGCTAAGTACCTTGTTTT
  CCTCAGTCCAGGGACATGGGAATGCAATAGATGAAATCTCCAAGTCCTTAATAAGTTTGA
  ATACCACATTGCTTGATTTGCAGCTCAACATAGAAAATCTGAATGGCAAATCCAAGAGAT
  ACCTTCAAACAACAAGAGGAAATCAGTAAATTAGAGGAGCGTGTTTACCATGTATCAGCA
  GAAATATGGCTATGAAAGAGGACAAGTGCATTTGGGACAGGAAATAAAGGAGAAGTGAAA
  GTTCCTGATACATCACTTATGATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138715 unedited
  GTTTTTTTTTTTAATGGGGAACCAAAGGCAATTNCAACATTNCCAAAGTAAATTAAGTCC
  TCAATTTGTATTGGTGCTATTAGGTTCTTTTCTTGACTGGACTGAACTTAGTAGATTCTC
  TCAAATACAAAAAGGATTTTTAGATTACTTATTAAAGATATATCTATGCATAAGTGGTAA
  ATCAGCATGCATATACCATACAGCAATATAGTATTAACAACCCATAGGCACTGATCTCAG
  ACATCCCAAGTGCCTATGGGTTGTCAATAGTACTATTAACAGTGTCATAGTACTAGTACT
  AGTAATAGTACTAGTAATAGCAATAGTACCAGTACTAGCAATAGTACTATTATTATTTTA
  AAGGTAAATTTTATTTTAAAATAAAAATAGTAATAGCAATAGTACTATTATTAGTGTTAA
  TAATACTAATAGTTAATACTAACAGTTAACACTAATAGTTAATACTATTGACAACCCATA
  GGCACTGATTTCAGACATCCCATGCATACCACTTACGCATAAGTAGTAAATCAGCATACA
  TATACCATACAGCAATACAGTATTAACAACCCAGAGGCACTGATCTCAGACATCCCAAAC
  AGGCTGACAATGCAAATGATTCTTTCAGAAACCATTGTATTTACCTTTTGAAGAAAATAC
  CTAGATAGTGGAACTGAACACAAAGAATTATTAACTAGCTGAAATTACAGCCAGGGTTTG
  AGACAGAAAGTGTACATTCAGCACTCATTCTCTTACCACAAAATATGCTGCCAGAGAAAA
  TAGCATGTTAACAAGTACACGATGTTCATGATAAAAGCATTTCAGTAGCTGCTTACAGCA
  TCCCAATTCACGAACGCTTGCCTTTTAGTTGGACGCTGGAGCCACCACTGTATATGCGGG
  GAGATTAGATAGAAAAAGCTCAGCTTTGTTCATTGCCCTTTAAGAGGACGGCCCGATTCT
  TTTAGGCCGTTAACATACTTCTGGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138715
• Insert Size: 3780 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138715.1, NP_619729.1
• RefSeq Size: 3682 bp
• RefSeq ORF: 1356 bp
• Locus ID: 4481
• Cytogenetics: 8p22
• Domains: SR, Macscav_rec, Collagen
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (SR-AI), also known as phSR1, encodes the longest isoform.